+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Quantitative ultrastructural changes in rat prolactin cells relationship to the rebound in serum prolactin levels after bromocriptine treatment



Quantitative ultrastructural changes in rat prolactin cells relationship to the rebound in serum prolactin levels after bromocriptine treatment



Acta Histochemica et Cytochemica 20(5): 537-546



Sprague-Dawley female rats were administered 10 mg/Kg of Bromocriptine by intubation daily for 3 weeks from 31 to 51 days of age. Tail blood was collected for RIA or PRL from meta- and di-estrus rats at the age of 51-55 days. Sampling was not repeated in any rat. A rebound increase in serum PRL levels occurred within 3 days after the cessation of subchronic treatment. Quantitative immunoelectron microscopy using the protein A gold method was carried out in some pituitary tissues from PRL-determined rats. Compared with the controls, excessive accumulation of higher gold-labeling mature secretory granules was found in PRL cells and also the Golgi complex was less developed in the treated rats just after the last intubation. The PRL cells exhibited more extrusion of secretory granules 2 days after the cessation when their ultrastructural features were almost completely restored. These data indicated that a rebound in serum PRL levels is accompanied by marked changes in the ultrastructural characteristics of the PRL cells.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 006246151

Download citation: RISBibTeXText

DOI: 10.1267/ahc.20.537


Related references

A broad spectrum of prolactin suppression by bromocriptine in hyperprolactinemic women: a study of serum prolactin and bromocriptine levels after acute and chronic admknistration of bromocriptine. Journal of Clinical Endocrinology and Metabolism 50(6): 1026-1033, 1980

Effects of bromocriptine on serum prolactin levels, pituitary weight and immunoreactive prolactin cells in estradiol-treated ovariectomized rats: an experimental model of estrogen-dependent hyperprolactinemia. Brazilian Journal of Medical and Biological Research 30(1): 113-117, 1997

Prolactin-secreting pituitary adenomas: serum and tissue prolactin levels with ultrastructural correlation. American Journal of Obstetrics and Gynecology 137(6): 646-652, 1980

Serum prolactin levels and the value of bromocriptine in the treatment of anovulatory infertility. British Journal of Obstetrics and Gynaecology 84(1): 58-66, 1977

Menstrual Function and Serum Prolactin Levels after Long-term Bromocriptine Treatment of Hyperprolactinaemic Amenorrhoea. Obstetric and Gynecologic Survey 38(7): 429-430, 1983

Calmodulin content in human prolactin-secreting pituitary adenoma: an inverse relationship to serum prolactin levels. Neuroscience Letters 90(1-2): 224-228, 1988

Dmba induced mammary carcinogenesis in relation to rebound increase of serum prolactin after suppression with bromocriptine in pubescent virgin rats. Journal of the Kansai Medical University 39(Suppl.): S-37-S-43, 1987

Menstrual function and serum prolactin levels after long term bromocriptine treatment of hyper prolactinemic amenorrhea. Clinical Endocrinology 16(6): 587-594, 1982

Studies on the immunologic potency of standard prolactin in different ria kits for prolactin and serum prolactin levels during the menstrual cycle. Mizuno, M , H Mori And Y Taketani (Ed ) Role Of Prolactin in Human Reproduction; 1st Symposium, Tokyo, Japan, October 4, 1985; 2nd Symposium, Tokyo, Japan, October 3, 1986; 3rd Symposium, October 2, 1987 X+313p S Karger Ag: Basel, Switzerland; New York, New York, Usa Illus 54-63, 1988

Hemodynamics, prolactin and catecholamine levels during hemorrhagic shock in dogs pretreated with a prolactin inhibitor (bromocriptine). Pharmacological Research Communications 19(4): 307-318, 1987

Nocturnal prolactin levels and prolactin release by metoclopramide in normoprolactinemic anovulation and effects of bromocriptine in induction of ovulation. Acta Obstetrica et Gynaecologica Japonica (Japanese Edition) 38(4): 603-604, 1986

Prolactin and testosterone: independent circulating levels in hyperprolactinemic and normoprolactinemic amenorrhea. The effect of prolactin suppression by bromocriptine. Journal of Clinical Endocrinology and Metabolism 43(1): 198-200, 1976

A comparison of the temporal effects of estradiol and diethylstilbestrol on pituitary content of DNA, prolactin mRNA and prolactin and on serum prolactin levels in ovariectomized Holtzman rats. Life Sciences 53(16): 1267-1272, 1993

Serum prolactin levels and prolactin mRNA expression in peripheral blood mononuclear cells in hepatitis C virus infection. Journal of Medical Virology 85(7): 1199-1205, 2013

Effects of bromocriptine treatment on prolactin, prolactin receptor, and immune function of calves on different photoperiods. Journal of Dairy Science 86(Suppl. 1): 103, 2003