+ Translate

Rabbit oocyte maturation changes of membrane resistance capacitance and the frequency of spontaneous transient depolarizations

, : Rabbit oocyte maturation changes of membrane resistance capacitance and the frequency of spontaneous transient depolarizations. Developmental Biology 120(1): 162-169

Immature oocytes from rabbits were examined with electrophysiological techniques to determine if their membrane properties change during maturation. The input resistance increased and input capacitance decreased during maturation, although no significant change in membrane potential was observed. The changes observed were consistent with a decrease of corona radiata-oocyte electrical coupling accompanying maturation. Spontaneous transient depolarizations were recorded from immature oocytes surrounded by corona radiata, but not from mature ova. Each event consisted of a rapid depolarization, sustained for 1-100 sec, and a slow repolarization to the resting potential. Spontaneous inward currents with a time course similar to the spontaneous transient depolarizations occurred when the oocyte's membrane potential was held constant by voltage clamp. The frequency with which spontaneous transient depolarizations occurred decreased during maturation. These findings are consistent with a model in which spontaneous depolarizations originate in corona radiata cells and are detected in the oocyte via electrical coupling.

Accession: 006247946

PMID: 3817287

DOI: 10.1016/0012-1606(87)90114-x

Download PDF Full Text: Rabbit oocyte maturation changes of membrane resistance capacitance and the frequency of spontaneous transient depolarizations

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Related references

McCulloch, D.H.; Levitan, H., 1987: Rabbit oocyte maturation: changes of membrane resistance, capacitance, and the frequency of spontaneous transient depolarisations. During oocyte maturation in vitro, input electrical resistance increased and input capacitance decreased, but no significant change in membrane potential was detected. The data were consistent with a decrease in electrical coupling between the cor...

Misro, M.M.; Chaube, S.K.; Chaki, S.P.; Guha, S.K., 2000: Spontaneous oocyte maturation in vitro Design and development of a system for testing compounds for toxicity or inhibition of oocyte maturation. Molecular & Cellular Endocrinology 164(1-2): 274, June 20

Barnett, D.; Misler, S., 1995: Nonlinear optimization applied to dual frequency estimation of membrane capacitance, resistance, and access resistance. Biophysical Journal 68(2 PART 2): A116

von der Weid, P-Yves.; Rahman, M.; Imtiaz, M.S.; van Helden, D.F., 2008: Spontaneous transient depolarizations in lymphatic vessels of the guinea pig mesentery: pharmacology and implication for spontaneous contractility. Guinea pig mesenteric lymphatic vessels exhibit rhythmic constrictions induced by action potential (AP)-like spikes and initiated by entrainment of spontaneous transient depolarizations (STDs). To characterize STDs and the signaling mechanisms res...

Rohlicek, V.; Schmid, A., 1994: Dual-frequency method for synchronous measurement of cell capacitance, membrane conductance and access resistance on single cells. A dual-frequency method was developed to monitor changes of membrane capacitance, membrane conductance and serial resistance between patch pipette and cytoplasm of the cell in the whole-cell configuration. Measurement of real and imaginary compone...

Nilius, B.; Boldt, W., 1984: Pacing-induced transient depolarizations in rabbit atrial myocardium. Transient afterdepolarizations (TD) were analyzed following a train of paced action potentials in isolated pectinate muscles bordering the crista terminalis in rabbit right atria. After cessation of a rapid drive TD peaked between 400 and 700 ms....

Kremers, M.S.; Kenyon, J.L.; Ito, K.; Sutko, J.L., 1985: Phenothiazine suppression of transient depolarizations in rabbit ventricular cells. We have examined the effects of trifluperazine and fluphenazine on action potentials and transient depolarizations of rabbit ventricular cells. Isolated myocytes were prepared by perfusing rabbit hearts with low calcium enzyme-containing solutions...

Berrios M.; Bedford J.M., 1979: Oocyte maturation aberrant post fusion responses of the rabbit primary oocyte penetrating spermatozoa. Biology of Reproduction 20(SUPPL 1): 129A

Berrios, M.; Bedford, J.M., 1979: Oocyte maturation: aberrant post-fusion responses of the rabbit primary oocyte to penetrating spermatozoa. Rabbit primary oocytes showing a germinal vesicle (GV oocytes) were placed in Fallopian tubes containing spermatozoa, and were recovered 2-5 h later for examination by light and electron microscopy. Although the vestments and oolemma seemed normal...

Kito, Y.; Sanders, K.M.; Ward, S.M.; Suzuki, H., 2010: Interstitial cells of Cajal generate spontaneous transient depolarizations in the rat gastric fundus. Intracellular recordings were made from isolated circular muscle bundles of rat gastric fundus. The majority of cells generated an ongoing discharge of electrical activity that were <or=10 mV in amplitude (unitary potentials). A second pattern...