+ Translate
+ Most Popular
Sobre os generos Lopadusa Stal e Bothrocoris Mayr (Hemiptera, Pentatomidae, Pentatomini)
RFEF pilot plant for inactivation of Escherichia coli in apple juice
Calpionella alpina Lorenz als "Leitfossil"
Sooretama: The Atlantic Rain Forest of Brazil. Francis Dov Por
Novel approaches in myeloma therapy
Preliminary Notes on the Study of Mitochondria During the 1st Stages of the Destruction of Cadavers
A preliminary study of the biology of lichee stink bug, Tessaratoma papulosa Drury, and its control
The status of the Sabu Island python - Die Situation des Savu-Pythons
Randomised trial of intravenous immunoglobulin as prophylaxis against infection in plateau-phase multiple myeloma. the UK Group for Immunoglobulin Replacement therapy in Multiple Myeloma
Conflicts between children
Clinical presentations in neuropsychiatry
Method of suturing a perforated ulcer of the stomach and duodenum
Identification of mechanical asphyxiation in cases of attempted masking of the homicide
Effects of endogenous ascorbate on oxidation, oxygenation, and toxicokinetics of cell-free modified hemoglobin after exchange transfusion in rat and guinea pig
Is it warm? Is it real? Or just low spatial frequency?
Additions and corrections to the list of Korean Heteroptera
Factors contributing to the maintenance of oligotrophy in an alpine glacial moraine lake (Wallowa lake) in Northeast Oregon
Classification, paleoecology, and biostratigraphy of crinoids from the Stull Shale (Late Pennsylvanian) of Nebraska, Kansas, and Iowa
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Neue Formen der Bauchsammler aus Turkmenien (Hymenoptera, Apoidea)
Atypische Hühnerpest und Newcastle-Krankheit. Ein Identifizierungsversuch
Parasitellus fucorum in the debris of Apis mellifera - a risk of confusion with Tropilaelaps spp
Applying the Technology Acceptance Model to the introduction of healthcare information systems
Ein akarologischer Nachruf für den "Roland"
Mutation research on crop plants in Argentina

Radioenzymatic assay for the acetohydroxy acid synthase-catalyzed synthesis of alpha-aceto-alpha-hydroxybutyrate

Radioenzymatic assay for the acetohydroxy acid synthase-catalyzed synthesis of alpha-aceto-alpha-hydroxybutyrate

Analytical Biochemistry 105(1): 101-105

ISSN/ISBN: 0003-2697

PMID: 6778247

DOI: 10.1016/0003-2697(80)90429-7

A radioenzymatic assay for the activity of acetohydroxy acid synthase [from Salmonella typhimurium] on 1 of its substrates, .alpha.-ketobutyrate, was developed. Advantage was taken of the fact that acidification of the product of the enzymatic reaction releases 14CO2, which is derived from the carboxyl group of .alpha.-ketobutyrate when .alpha.-[1-14C]ketobutyrate is used as the substrate. The 14CO2 evolved is collected and measured in a simple apparatus which is described.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 006250481

Download citation: RISBibTeXText

Related references

Stereochemistry of valine and iso leucine biosynthesis 4. synthesis configuration and enzymatic specificity of alpha aceto lactate and alpha aceto alpha hydroxy butyrate. Bioorganic Chemistry 8(2): 175-190, 1979

Biosynthesis of valine and iso leucine synthesis and biological activity of 2 s alpha aceto lactic acid 2 hydroxy 2 methyl 3 oxo butanoic acid and 2 r alpha acetohydroxy butyric acid and 2 s alpha acetohydroxy butyric acid 2 ethyl 2 hydroxy 3 oxo butanoic acid. Journal of the Chemical Society Perkin Transactions I (6): 1197-1202, 1983

De repression of valine sensitive aceto hydroxy acid synthase ec by threonine alpha keto butyrate or iso leucine in escherichia coli k 12. Japanese Journal of Genetics 57(5): 483-498, 1982

Substrate range of acetohydroxy acid synthase I from Escherichia coli in the stereoselective synthesis of alpha-hydroxy ketones. Biotechnology and Bioengineering 88(7): 825-831, 2004

Leucine biosynthesis in alcaligenes eutrophus h 16 influence of amino acid additions on the formation of active alpha isopropylmalate synthase and alpha acetohydroxy acid synthase. Archives of Microbiology 142(2): 194-199, 1985

Enzymatic synthesis of alpha-butylglucoside lactate: a new alpha-hydroxy acid derivative. Biotechnology and Bioengineering 62(2): 225-234, 1999

Effects of some drugs on the metabolism of 5 hydroxy tryptamine and on the survival of x irradiated rats iproniazid radio protect reserpine radio protect alpha methyl dopa radio protect 5 hydroxy tryptophan radio protect 5 hydroxy indole acetic acid radio protect. Iugoslavica Physiologica Pharmacologica Acta 3(2): 137-145, 1967

Synthesis of chiral alpha-hydroxy amides by two sequential enzymatic catalyzed reactions. Applied Microbiology and Biotechnology 75(2): 297-302, 2007

Enzymatic hydrolysis of conjugated 3 alpha 7 alpha 12 alpha tri hydroxy 5 beta cholestan 26 oic acid absolute configuration in alligator bile. Federation Proceedings 38(3 Part 1): 706, 1979

Novel synthesis of alpha-trifluoromethylated alpha-amino acid derivatives from gamma-hydroxy-alpha-fluoro-alpha-trifluoromethyl carboxamides. Organic Letters 7(4): 589-592, 2005

Enantioselective Synthesis of alpha,alpha-Disubstituted Amino Acid Derivatives via Enzymatic Resolution: Preparation of a Thiazolyl-Substituted alpha-Methyl alpha-Benzyl Amine. Journal of Organic Chemistry 61(21): 7398-7401, 1996

Synthesis of the allylic gonadal steroids, 3 alpha-hydroxy-4-pregnen-20-one and 3 alpha-hydroxy-4-androsten-17-one, and of 3 alpha-hydroxy-5 alpha-pregnan-20-one. Steroids 45(1): 39-51, 1985

Phase-transfer-catalyzed synthesis of aryl alpha-ketosides of N-acetylneuraminic acid. A 2-methylfluoran-6-yl glycoside of N-acetylneuraminic acid, 2-methyl-6-(5-acetamido-3,5-dideoxy-alpha-D-glycero-D-galacto- nonulopyranosylonic acid)xanthene-9-spiro-1'-isobenzofuran-3'-one, a new substrate for neuraminidase assay. Carbohydrate Research 196: 29-40, 1990

Regulation of synthesis of the aceto hydroxy acid synthase i iso enzyme in escherichia coli k 12. Biochimica et Biophysica Acta 541(1): 1-8, 1978

Substrate Range of Acetohydroxy Acid Synthase I from Escherichia coli in the Stereoselective Synthesis of a-Hydroxy Ketones. Biotechnology and Bioengineering 88(7): 5-31, 2004