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Radio immunoassay of acth from plasma

Radio immunoassay of acth from plasma

Clinical Chemistry 28(11): 2229-2234

A specific and highly sensitive radioimmunoassay for ACTH in human plasma, is described. As little as 1.9 pg of circulating ACTH can be detected/ml (0.44 pmol/l). ACTH is first extracted from plasma samples by adsorption onto pretreated Vycor glass powder, eluted from the adsorbant by acetone, and then lyophilized. For purification of 125I-labeled ACTH, a small octadecasilyl silica column is used. The mean (and SD) concentration of ACTH in plasma from 18 ostensibly normal subjects was 10.3 (4.3) pmol/l. After overnight suppression with dexamethasone in seven of these normal subjects, their ACTH values fell to 2.62 (1.3) pmol/l (P < 0.0005). This assay will permit clinical evaluation of ACTH plasma concentrations in clinical investigation and of the effects of drug administration on circulating ACTH.

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Accession: 006250919

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