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Raman and ir studies of homogeneous forms of acid phosphatase ec from rat liver

, : Raman and ir studies of homogeneous forms of acid phosphatase ec from rat liver. Biochimica et Biophysica Acta 790(1): 70-77

Raman spectra of acid phosphatase forms from rat liver in water solution, and IR spectra of the same forms as thin films, were investigated. The spectra show strong bands belonging to phosphodiester or phosphomonoester residues. These groups are modified during the postsynthetic modification of acid phosphatase and are probably connected with the process of bonding and splitting of mannose 6-phosphate and N-acetylglucosamine, in agreement with previous biochemical models for the intracellular transport of newly synthesized lysosomal hydrolases to lysosomes. Some other bands in the IR spectra are assigned to vibrations of N-H groups which may belong to N-acetylglucosamine.

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