+ Translate

Raman ir spectroscopic study of gramicidin a conformations

, : Raman ir spectroscopic study of gramicidin a conformations. Archives of Biochemistry & Biophysics 202(2): 639-649

Raman scattering and IR spectroscopic techniques were used to study the vibrational spectrum and conformation of the membrane channel protein gramicidin A in the solid state, in organic solutions and, using Raman scattering only, in a phospholipid environment. The investigation includes measurements on head- and tail-group modified gramicidin A and a potassium thiocyanate-gramicidin A complex. Tentative identification of the molecular vibrations is proposed on the basis of the data on model compounds. The existence of 4 distinct conformations of the gramicidin A chain is established: conformation I present in the solid state, and CH3OH and CD3OD solutions; conformation II present in films cast from CHCl3 solution; conformation III present in (CH3)2SO and (CD3)2SO solutions at concentrations below 0.5 M gramicidin A; and conformation IV present in the potassium thiocyanate-gramicidin A complex. The data obtainable on a gramicidin A-phospholipid suspension indicate a gramicidin A conformation in this environment corresponding either to the conformation I or II. The details of the spectra in the amide I region are consistent with a .beta.-parallel H-bonded .pi.LD helix for conformational I, in terms of the polypeptide vibrational calculations of Nevskaya and co-workers. Conformation II is consistent with an antiparallel double-stranded .pi.LD helix, while conformations III and IV probably have .pi.-helical structures with larger channel diameters. The data on head- and tail-modified gramicidin A molecules indicate that their confrmations are only slightly different from that of gramicidin A in conformation I.

(PDF 0-2 workdays service)

Accession: 006255772

Submit PDF Full Text: Here

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Related references

Iqbal, Z.; Weidekamm, E., 1980: Raman and infrared spectroscopic study of gramicidin A conformations. Archives of Biochemistry and Biophysics 202(2): 639-649

Rothschild, K.J.; Stanley, H.E., 1974: Raman spectroscopic investigation of gramicidin A' conformations. Gramicidin A' is believed to form transmembrane channels in lipid bilayers and biological membranes. The first Raman spectroscopic study of gramicidin A' is presented. Evidence is found for two types of conformation. One type is found in...

Rothschild K.J.; Stanley H.E., 1974: Raman spectroscopic investigation of gramicidin a prime conformations. Science (Washington D C) 185(4151): 616-618

Katahira, M.; Nishimura, Y.; Tsuboi, M.; Matsuzaki, J.-Ichi; Hotoda, H.; Sekine, M.; Hata, T., 1994: A raman spectroscopic study on conformations of DNA oligomers: A dominant effect of an AA:TT sequence over those of AT:AT and TA:TA sequences on determining conformations of DNA duplexes. The conformations (of the following ten DNA oligomers in solution have been studied by a Raman spectroscopy; d(ATAT)-2, d(TATA)-2, d(AATT)-2, d(TTAA)-2, d(TTATATAA)-2, d(AATTAATTAATT)-2 (dodecamer 1), d(CGCGATATCGCG)-2, d(CGCGTATACGCG)-2, D(CGCGAA...

Nishimura, Y.; Torigoe, C.; Katahira, M.; Tate, S.; Tanaka, K.; Tsuboi, M.; Matsuzaki, J.; Hotoda, H.; Sekine, M.; Hata, T., 1986: A Raman spectroscopic study on the sequence dependent conformations of DNA oligomers. Eighteen kinds of oligodeoxyribonucleic acids have been examined to reveal their structures in aqueous solutions at different ionic strengths by Raman spectroscopy. The structures in solutions were found to be very polymorphic depending on their s...

Bouchard, M.; Auger, M., 1993: Solvent history dependence of gramicidin-lipid interactions: a Raman and infrared spectroscopic study. We have investigated the interactions between gramicidin and a model membrane composed of one phospholipid, dimyristoylphosphatidylcholine, as a function of the cosolubilization solvent and incubation time used in the sample preparation. Three org...

Bouchard, M.; Auger, M., 1993: Solvent history dependence of gramicidin-lipid interactions A Raman and FTIR spectroscopic study. Biophysical Journal 64(2 PART 2): A61

Aslanian, D.; Négrerie, M.; Chambert, R., 1986: A Raman spectroscopic study on the interaction of an ion-channel protein with a phospholipid in a model membrane system (gramicidin A/L-alpha-lysophosphatidylcholine). The interaction of the ion channel polypeptide gramicidin A with the L-alpha-lysophosphatidylcholine micelles in a membrane state association (approximative molar ratio 1:9) was investigated by Raman spectroscopy. Studies were carried out over the...

Benevides J.M.; Wang A.H J.; Thomas G.J.Jr, 1987: Effect of the gt wobble base pair on z dna and b dna conformations a raman spectroscopic study of the self complementary dna hexamer d cgcgtg. Biophysical Journal 51(2 PART 2): 504A

Takeuchi, H.; Nemoto, Y.; Harada, I., 1990: Environments and conformations of tryptophan side chains of gramicidin A in phospholipid bilayers studied by Raman spectroscopy. Raman spectroscopy has been used to investigate the hydrophobic interaction of the indole ring with the environments, the water accessibility to the N1H site, and the conformation about the C beta-C3 bond for the four tryptophan side chains of gra...