+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Raman spectroscopic elucidation of dna backbone conformations for polydeoxyguanylic polydeoxythymidylic acid polydeoxyadenylic polydeoxycytidylic acid and double stranded polydeoxyadenylic polydeoxythymidylic acid in cesium fluoride solution

, : Raman spectroscopic elucidation of dna backbone conformations for polydeoxyguanylic polydeoxythymidylic acid polydeoxyadenylic polydeoxycytidylic acid and double stranded polydeoxyadenylic polydeoxythymidylic acid in cesium fluoride solution. Biopolymers 24(8): 1493-1500

Raman spectra have been recorded for poly([deoxy]dG-dT) .cntdot. poly(dA-dC) and poly(dA-dT) .cntdot. poly(dA-dT) in low salt and at high concentrations of CsF. Poly(dG-dT) .cntdot. poly(dA-dC) shows no change in the 682/cm guanine mode, demonstrating the absence of the Z-structure at high salt. The 790-cm-1 phosphodiester symmetric stretch, however, shifts up 5/cm in 4.3 M CsF, suggesting a slight conformational change, associated with ion binding or hydration changes. Poly(dA-dT) .cntdot. poly(dA-dT) shows an additional broad band at 816/cm, attributed to the phosphodiester modes associated with the C3'-endo deoxyribose units in the alternating B-structure. In this case, both the 841- and the 816/cm asymmetric phosphodiester stretches, associated with the C2'- and C3'-endo units, shift down on addition of CsF in a sequential manner. Correlation of this sequence with that previously observed for the 2 31P-NMR resonances, establishes that the phosphodiester stretching frequencies depend on the conformation of the 5'-sugar, and not on the 3'-sugar.

(PDF 0-2 workdays service)

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 006255828

PDF Full Text: Submit

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Rizzo P.J.; Bradley W.; Morris R.L., 1985: Histones of the unicellular alga olisthodiscus luteus. The 4 major histones of the marine unicellular alga O. luteus were prepared from isolated nuclei and fractionated by molecular exclusion chromatography and preparative gel electrophoresis. The isolated histones were characterized by amino acid ana...

Anonymous, 2012: The association of cytokine gene polymorphism with reflux nephropathy. To identify genetic risk factors for the progression of vesicoureteral reflux (VUR) to reflux nephropathy, we examined polymorphisms of multiple cytokine genes among VUR patients with or without renal scarring. A total of 238 VUR patients aged bet...

Jorgensen L.G.; Perko M.; Hanel B.; Schroeder T.V.; Secher N.H., 1992: Middle cerebral artery flow velocity and blood flow during exercise and muscle ischemia in humans. Changes in middle cerebral artery flow velocity (Vmean), measured by transcranial Doppler ultrasound, were used to determine whether increases in mean arterial pressure (MAP) or brain activation enhance cerebral perfusion during exercise. We also...

Daro D., 1991: Child sexual abuse prevention separating fact from fiction. Child Abuse & Neglect 15(1-2): 1-4

Mark E. Hines; Ramona E. Pelletier; Patrick M. Crill, 1993: Emissions of sulfur gases from marine and freshwater wetlands of the Florida Everglades; rates and extrapolation using remote sensing. Journal of Geophysical Research: Atmospheres 98(D5): 8991-8999

Haruta T.; Ohkura K E.; Kuroki S.; Yamamoto H.; Yoshioka M.; Kobayashi Y., 1986: Clinical evaluation of ceftazidime in infections in neonates and premature infants. Ceftazidime (CAZ) was evaluated in the treatment of infections in neonates and premature infants. 1. In 1 mature neonage (age: 14 days) administered with 30.3 mg/kg of CAZ by a single bolus intravenous injection, the serum concentration of CAZ was...

Snutch, Terrance Preston; Zamponi, Gerald Werner, 2001: Calcium channel blockers. Compounds of the formula ##STR1## wherein m is 0, 1 or 2; wherein when m is O, Z is O, when m is 1, Z is N, and when m is 2, Z is C; Y is H, OH, NH2, or an organic moiety of 1-20C, optionally additionally containing 1-8 heteaoms selected from te g...

Vannucci, M.; Navas, D., 1973: Distribution of hydromedusae in the Indian Ocean. Unknown

Stoesz, Paul A.; Lepage, G. A., 1949: Glycolysis in liver homogenates. A medium for anaerobic glycolysis of rat liver, containing hexose diphosphate as the substrate, has been developed. The [image] obtained was approx. 78. Phosphate bond energy was maintained at satisfactory levels.

Anonymous, 2013: Natural Flora and Anticancer Regime Milestones and Roadmap. Cancer has long been an area of extensive research both at the molecular as well as pharmaceutical level. However, lack of understanding of the underlying molecular signalling and the probable targets of therapeutics is a major concern in successf...