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Raman spectroscopic elucidation of dna backbone conformations for polydeoxyguanylic polydeoxythymidylic acid polydeoxyadenylic polydeoxycytidylic acid and double stranded polydeoxyadenylic polydeoxythymidylic acid in cesium fluoride solution


Biopolymers 24(8): 1493-1500
Raman spectroscopic elucidation of dna backbone conformations for polydeoxyguanylic polydeoxythymidylic acid polydeoxyadenylic polydeoxycytidylic acid and double stranded polydeoxyadenylic polydeoxythymidylic acid in cesium fluoride solution
Raman spectra have been recorded for poly([deoxy]dG-dT) .cntdot. poly(dA-dC) and poly(dA-dT) .cntdot. poly(dA-dT) in low salt and at high concentrations of CsF. Poly(dG-dT) .cntdot. poly(dA-dC) shows no change in the 682/cm guanine mode, demonstrating the absence of the Z-structure at high salt. The 790-cm-1 phosphodiester symmetric stretch, however, shifts up 5/cm in 4.3 M CsF, suggesting a slight conformational change, associated with ion binding or hydration changes. Poly(dA-dT) .cntdot. poly(dA-dT) shows an additional broad band at 816/cm, attributed to the phosphodiester modes associated with the C3'-endo deoxyribose units in the alternating B-structure. In this case, both the 841- and the 816/cm asymmetric phosphodiester stretches, associated with the C2'- and C3'-endo units, shift down on addition of CsF in a sequential manner. Correlation of this sequence with that previously observed for the 2 31P-NMR resonances, establishes that the phosphodiester stretching frequencies depend on the conformation of the 5'-sugar, and not on the 3'-sugar.

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Accession: 006255828



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