+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Raman spectroscopic study of saturated mixed chain phosphatidyl choline multi lamellar dispersions

, : Raman spectroscopic study of saturated mixed chain phosphatidyl choline multi lamellar dispersions. Biophysical Journal 41(2 PART 2): 350A

The thermotropic behavior of a series of mixed-chain saturated phospholipids in fully hydrated multilamellar dispersions was examined by vibrational Raman spectroscopy in both the C.sbd.C stretching (1000-1200-cm-1) and the C.sbd.H stretching (2800-3100-cm-1) mode regions. This lipid series represents phosphatidylcholines [PC] in which the sn-1 acyl chain is fixed in length with 18 C atoms, while the sn-2 acyl chain length is increased in steps of 2 methylene units from 10 to 18 C atoms. The transition temperatures of these mixed-chain PC measured by Raman spectroscopic procedures are in good agreement with those determined by high-sensitivity differential scanning calorimetry. The total change in the Raman spectral intensity ratio .DELTA.(I2935cm-1/I2884cm-1) across the narrow gel .tautm. liquid-crystalline phase transition temperature region is markedly different for the mixed-chain phospholipids in comparison to the symmetric chain systems. For the mixed-chain bilayer dispersions, the data conform to a smooth hyperbolic cosine curve and vary in the order C(18):C(10)PC > C(18):C(12)PC > C(18):C(14)PC < C(18):C(16)PC < C(18):C(18)PC with a minimum chain-chain order/disorder change observed for the C(18):C(14)PC multilamellar assemblies. The change in the spectral intensity ratio determined in the 1000-1200-cm-1 region is similar to that detected in the 2800-3100-cm-1 region; the smallest change in the spectral intensity ratio .DELTA.(I1088cm-1/I1065cm-1) across the phase transition region is again observed for the C(18):C(14)PC dispersions. These data, together with other structural information determined at common reduced temperatures for various gel state, mixed-chain phospholipid dispersions, lead to the conclusion that membrane lipids adopt 2 types of chain conformations in bilayer systems. In the first class, the lipid molecules in the 2 opposing monolayers pack independently, as typified by the C(18):C(18)PC and C(18):C(16)PC bilayers. In contrast, the hydrocarbon chains in the second type of lipid bilayers are assumed to effect an interdigitated packing arrangement which couples the 2 monolayers across the bilayer center. This second type of bilayer is represented by the C(18):C(10)PC, C(18):C(12)PC, and C(18):C(14)PC dispersions.

(PDF 0-2 workdays service)

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 006255845

PMID: 6871161

PDF Full Text: Submit

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Winzenburg, S.M.; Imola, M.J., 1999: Internal fixation in pediatric maxillofacial fractures. Pediatric facial trauma presents unique problems in diagnosis and management. The following review highlights relevant points of craniofacial growth and applied anatomy. The epidemiology of pediatric facial fractures is presented followed by perti...

House, J.S.; Wyss, A.B.; Hoppin, J.A.; Richards, M.; Long, S.; Umbach, D.M.; Henneberger, P.; Beane Freeman, L.E.; Sandler, D.P.; Long O'Connell, E.; Barker Cummings, C.; London, S.J., 2016: Early-life Farm Exposures and Adult Asthma and Atopy in the Agricultural Lung Health Study. Journal of Allergy and Clinical Immunology (): -

Rybinskii, Iuv; Kitaev, Av; Vasilevskii, Vd, 1973: Measuring the electrification of farm electroaerosols. Aerozoli v Sel'skom Khoziaistve: 61-165

Winiarz, J.G.; Ghebremichael, F., 2004: Beam cleanup and image restoration with a photorefractive polymeric composite. We demonstrate, for the first time to our knowledge, the use of a photorefractive polymeric composite to clean a phase-distorted laser beam and reconstruct a badly distorted image. Advantageous qualities including relatively high figures of merit,...

Albrightson Winslow C.R.; Brickson B.B.; Hutchman J.; Badger A., 1990: Beneficial effects of azaspirine mrl mp 1pr 1pr mrl mice which spontaneously develop autoimmune disease. Kidney International 37(1): 406

Villee D.B., 1969: Development of endocrine function in the human placenta and fetus 2nd of 2 parts. New England Journal of Medicine 281(10): 533-541

Bedi Yavuz; Usta Dogan; Usta Metin; Ozturk Esat Melih, 2004: Manavgat (Antalya)-Anamur (Mersin) arasindaki alanin stratigrafik-yapisal ozellikleri (Orta Toroslar)--The stratigraphical and structural characteristics of the area between Manavgat (Antalya)-Anamur (Mersin), central Taurides. Turkiye Jeologi Kurultayi Bildiri Ozleri = Abstract of the Geological Congress of Turkey 57: 277-278

Chen, C-Yuen.; Yao, M-Szu.; Chan, W.P., 2010: Subacute post-traumatic diffuse axonal injury presenting on MRI as multiple enhancing nodules. Acta Neurologica Belgica 110(2): 213-213

Park, E. K.; La, Y. J.; Heu, I.; Lee, Y. D., 1975: Serological investigation of virus diseases of tobacco plant (Nicotiana tabacum L.) in Korea. Tobacco mosaic, cucumber mosaic, alfalfa mosaic, potato X and potato Y viruses occurred on tobacco grown in Korea, prevalence among 40 collected plants being 47.5, 60, 67.5, 10 and 17.5%, respectively. In Burley tobacco TMV infection was 15% where...

Nelson, Eva R.; Nelson, Peter F.; Samuelson, Olof, 1968: A new methylated uronic acid from paper pulp hydrolysates. A methylated uronic acid of unknown structure was an important component of the acids of birth eucalypt and pine sulfate pulp, and eucalypt neutral sulphite pulp. The new acid was formed by some rearrangement of the 4-O-methyl-D-glucuronic acid un...