Rapid quantitative specific measurement of pancreatic amylase ec 3.2.1.1 in serum with use of a monoclonal antibody

Mifflin, T.E.; Benjamin, D.C.; Bruns, D.E.

Clinical Chemistry 31(8): 1283-1286

1985


ISSN/ISBN: 0009-9147
Accession: 006259869

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Abstract
In this rapid quantitative assay for pancreatic .alpha.-amylase (EC 3.2.1.1) in serum were 1.3% within-run, 6-8% day-to-day. Apparent analytical recovery of pancreatic amylase activity added to serum was 101% .+-. 2%. Addition of purified salivary amylse, 356 U/l, to sera gave a value for apparent pancreatic amylase of < 4 U/l, or 1% of the added salivary amylase activity. This assay correlated well with an electrophoretic method (slope, 0.97-0.99; intercept, 0.5-4 U/l; correlation coefficient, 0.946-0.990; and SE of the estimate 3-5 U/l). Estimated normal reference intervals with maltotetraose as substrate were: total amylase, 39-118 U/l; pancreatic amylase, 11-50 U/l; and salivary amylase, 18-79 U/l.