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Reconstitution of ATPase from the isolated subunits of coupling factor F1's of Escherichia coli and thermophilic bacterium PS3

Futai, M.; Kanazawa, H.; Takeda, K.; Kagawa, Y.

Biochemical and Biophysical Research Communications 96(1): 227-234

1980

ISSN/ISBN: 0006-291X
PMID: 6449197
DOI: 10.1016/0006-291x(80)91204-8
Accession: 006272518
ATPase was reconstituted from mixtures of isolated subunits of coupling factor, F1 ATPase of E. coli (EF1) and thermophilic bacterium PS3 (TF1); ability of hydrolyze ATP was attained from the combination of .alpha. and .beta. subunits from EF1 and .gamma. subunit from TF1, .alpha. and .beta. from TF1 and .gamma. from EF1, and .alpha. and .gamma. from EF1 and .beta. from TF1. The .beta. subunit of TF1 also could complement the EF1 from an E. coli mutant defective in this subunit. This is the 1st demonstration of interspecies in vitro recombination of ATPase activity from isolated subunits.

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Related References

Takeda, K.; Hirano, M.; Kanazawa, H.; Nukiwa, N.; Kagawa, Y.; Futai, M. 1982: Hybrid ATPase's formed from subunits of coupling factor F1's of Escherichia coli and thermophilic bacterium PS3 Journal of Biochemistry 91(2): 695-701
Takeda, K.; Hiano, M.; Kanazawa, H.; Nukiwa, N.; Kagawa, Y.; Futai, M. 1982: Hybrid atpase species formed from subunits of coupling factor f 1 of escherichia coli and thermophilic bacterium ps 3 Journal of Biochemistry 91(2): 695-702
Dunn, S.D.; Futai, M. 1980: Reconstitution of a functional coupling factor from the isolated subunits of Escherichia coli F1 ATPase Journal of Biological Chemistry 255(1): 113-118
Futai, M. 1977: Reconstitution of ATPase activity from the isolated alpha, beta, and gamma subunits of the coupling factor, F1, of Escherichia coli Biochemical and Biophysical Research Communications 79(4): 1231-1237
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Kagawa, Y.; Yoshida, M. 1979: Soluble ATPase (F1) from a thermophilic bacterium: purification, dissociation into subunits, and reconstitution from individual subunits Methods in Enzymology 55: 781-787
Vogel, G.; Steinhart, R. 1976: ATPase of Escherichia coli: purification, dissociation, and reconstitution of the active complex from the isolated subunits Biochemistry 15(1): 208-216
Dunn, S.D.; Heppel, L.A. 1981: Properties and functions of the subunits of the Escherichia coli coupling factor ATPase Archives of Biochemistry and Biophysics 210(2): 421-436
Futai, M.; Kanazawa, H. 1979: Dissociation and reconstitution of escherichia coli coupling factor 1 atpase analysis of the defect in uncoupled mutants Mukohata, Y And L Packer (Ed ) Cation Flux Across Biomembranes;proceedings Of A Symposium, Kobe, Japan, Sept 10-13, 1978 Xx+444p Academic Press: New York, N Y , Usa; London, England Illus 291-298
Kagawa, Y.; Sone, N. 1979: DCCD-sensitive ATPase (TF0 . F1) from a thermophilic bacterium: purification, dissociation into functional subunits, and reconstitution into vesicles capable of energy transformation Methods in Enzymology 55: 364-372
Rögner, M.; Gräber, P. 1986: Spectroscopic characterization of the ATPase of the thermophilic bacterium PS3 and its isolated subunits Journal of Biochemistry 99(4): 993-1003
Kagawa, Y. 1979: Protonophoric action of coupling factor atpase its reconstitution from purified subunits reconstitution into atp synthesizing vesicles and image reconstruction Schaefer, G And M Klingenberg (Ed ) Colloquium Der Gesellschaft Fuer Biologische Chemie (Colloquium Of The Society For Biological Chemistry), Vol 29 Energy Conservation in Biological Membranes, Apr 6-8, 1978 Xiv+287p Springer-Verlag: New York, N Y , Usa; Berlin, West Germany Illus P195-219, 1978
Roegner, M.; Luecken, U.; Tiedge, H.; Weber, J.; Graeber, P. 1985: Ligand binding to isolated subunits and the native proton translocating atpase of the thermophilic bacterium ps 3 Schaefer, G (Ed ) Icsu (International Council Of Scientific Unions) Short Reports, Vol 3 Third European Bioenergetics Conference; Hannover, West Germany, Sept 2-7, 1984 Xlviii+745p Cambridge University Press: New York, N Y , Usa; Cambridge, England Illus 356-358
Bar-Zvi, D.Y.shida, M.S.avit, N. 1985: Reconstitution of photophosphorylation with coupling factor 1 ATPases from the thermophilic bacterium PS3 and lettuce chloroplasts Biochimica et biophysica acta: bioenergetics 806(3): 341-347
Wakabayashi, T.; Kubota, M.; Yoshida, M.; Kagawa, Y. 1977: Structure of ATPase (coupling factor TF1) from a thermophilic bacterium Journal of Molecular Biology 117(2): 515-519