EurekaMag.com logo
+ Site Statistics
References:
53,623,987
Abstracts:
29,492,080
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Reconstruction of the contused cat spinal cord by the delayed nerve graft technique and cultured peripheral nonneuronal cells



Reconstruction of the contused cat spinal cord by the delayed nerve graft technique and cultured peripheral nonneuronal cells



Acta Neuropathologica 57(1): 59-69



The results of surgical reconstruction of the severely contused cat spinal cord by the delayed nerve graft technique alone were compared to those after reconstruction with a similar nerve graft plus cultured peripheral nonneuronal cells implanted between the grafted nerve and the spinal cord stumps. The spinal cord-nerve graft junction was examined by light microscopy and EM. The cultured cells were prelabeled with tritiated thymidine and their location after implantation determined by autoradiography. By 3 days after spinal cord reconstruction, the prelabeled cells were present at the junction and had migrated into the nerve graft and into the spinal cord stumps where they were observed near axons. By 7 days physical connections were observed bridging the junction between the spinal cord and nerve graft, and axons ensheathed by Schwann cells had already penetrated at least 1 mm into the nerve graft. Wound healing took at least a week longer in animals repaired with a nerve graft alone. At 1 yr or later after reconstructive surgery, in both groups of animals the grafted nerve was reinnervated with myelinated and unmyelinated axons. The severely contused cat spinal cord could be reconstructed with the delayed nerve graft technique alone but the use of the cultured cells appeared to enhance wound healing and decrease the time required for axon elongation into the nerve graft.

Accession: 006273121

Download citation: RISBibTeXText

PMID: 7090743

DOI: 10.1007/bf00688878

Download PDF Full Text: Reconstruction of the contused cat spinal cord by the delayed nerve graft technique and cultured peripheral nonneuronal cells



Related references

Observation of cultured peripheral nonneuronal cells implanted into the transected spinal cord. Acta Neuropathologica 64(3): 203-212, 1984

Preparation of large quantities of nonneuronal cells from peripheral nervous tissue for spinal cord reconstruction. Kao, C C , R P Bunge And P J Reier (Ed ) Spinal Cord Reconstruction; 1st International Symposium, Las Vegas, Nevada, Usa, Jan 1980 Xix+490p Raven Press: New York, N Y , Usa Illus P317-328, 1983

Bone marrow stromal cells elicit tissue sparing after acute but not delayed transplantation into the contused adult rat thoracic spinal cord. Journal of Neurotrauma 26(12): 2313-2322, 2010

Muscle reinnervation with delayed or immediate transplant of embryonic ventral spinal cord cells into adult rat peripheral nerve. Cell Transplantation 11(3): 241-250, 2002

Selected gene profiles of stressed NSC-34 cells and rat spinal cord following peripheral nerve reconstruction and minocycline treatment. Experimental and Therapeutic Medicine 11(5): 1685-1699, 2016

Transplantation of cultured xenogenic schwann cells into peripheral nerve and spinal cord of immuno suppressed mice. Kao, C C , R P Bunge And P J Reier (Ed ) Spinal Cord Reconstruction; 1st International Symposium, Las Vegas, Nevada, Usa, Jan 1980 Xix+490p Raven Press: New York, N Y , Usa Illus P305-316, 1983

Effects of prelesioned peripheral nerve graft on nerve regeneration in the rat spinal cord. Neurosurgery 42(6): 1347-1356, 1998

Central peripheral delayed neuropathy caused by dfp segregation of peripheral nerve and spinal cord effects using biochemical clinical and morphological criteria. Toxicology & Applied Pharmacology 88(1): 87-96, 1987

Using acidic FGF in peripheral nerve graft for spinal cord injury. Journal of Neurotrauma 15(1): 6, 1998

Reconstruction of the injured spinal cord/PNS interface of adult rats with collagen channels connected to nerve roots or peripheral nerve grafts. Society for Neuroscience Abstracts 23(1-2): 1681, 1997

Central-peripheral delayed neuropathy caused by diisopropyl phosphorofluoridate (DFP): segregation of peripheral nerve and spinal cord effects using biochemical, clinical, and morphological criteria. Toxicology and Applied Pharmacology 88(1): 87-96, 1987

Increased nerve growth factor receptor mRNA in contused rat spinal cord. Neuroscience Letters 118(2): 238-240, 1990

Increased nerve growth factor receptor messenger rna in contused rat spinal cord. Society for Neuroscience Abstracts 16(1): 825, 1990

The structure of nerve elements in the brain and spinal cord of rabbits following hypothermia, visualized with the light microscope. II. Morphology of nerve cells in the spinal cord, nerve fibres in the spinal cord and brain. Bratislavske Lekarske Listy 66(5): 541-549, 1976

Axonal regrowth of the adult spinal cord neurons of the dog into a peripheral nerve graft. Recueil de Medecine Veterinaire de l'Ecole d'Alfort 171(8-9): 559-565, 1995