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Recovery by push pull perfusion on neuro chemicals released within the cuneate nucleus of the cat by somato sensory stimulation



Recovery by push pull perfusion on neuro chemicals released within the cuneate nucleus of the cat by somato sensory stimulation



Pharmacology Biochemistry & Behavior 21(1): 117-124



A combination of techniques is described for the identification of neurochemicals released within the cuneate nucleus. During electrical stimulation of the superficial radial nerve, the extracellular fluid of the nucleus is continuously sampled by push-pull perfusion. In addition, the population electrical activity of peripheral nerve as well as the activity of cuneate neurons are reocrded. Subsequently, the neurochemical content of the sampled fluid is assessed by HPLC [high performance liquid chromatography] analysis. The comparison of sampled fluid content during control (no stimulation) vs. stimulation runs indicates that somatosensory stimulation elicits the release of specific neurochemicals within the cuneate nucleus. The possible sources of released neurochemicals are discussed.

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Accession: 006273843

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PMID: 6146990



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