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Regions of dna sequence homology between an octopine and a nopaline t i plasmid of agrobacterium tumefaciens



Regions of dna sequence homology between an octopine and a nopaline t i plasmid of agrobacterium tumefaciens



Molecular & General Genetics 169(2): 163-172



Despite the fact that pTiC58 and pTiB6S3 functionally were shown to have only tumorigenicity and phage AP1 exclusion in common, many restriction fragments of the plasmids contain DNA sequences common to both. The bulk of this homologous DNA is concentrated in a few restriction endonuclease fragments and the remainder is organized in short discontinuous regions spread over many fragments. In pTiB6S3 the bulk of the homology is distributed throughout a 29 .times. 106 dalton segment comprising 8 Sma I fragments. This region includes those sequences which are transferred to and transcribed in tumorigenic plant cells induced by B6-806 or closely related strains. The pattern of homology within this portion of the plasmid shows a region of low sequence homology (Sma I fragment 3b) apparently corresponding to the gene or genes coding for octopine synthesis in the plant tumor cells, surrounded by regions of high sequence homology. The extent of inter-plasmid homology then decreases with increasing distance from fragment 3b. The remainder of the homology is distributed throughout a segment of maximum size 21.5 .times. 106 daltons comprising 2 Sma I fragments and cannot yet be definitely linked with any specific plasmid function.

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