Regulation of cell wall synthesis by auxin and fusicoccin in different tissues of pea pisum sativum cultivar alaska stem segments

Brummell, D.A.; Hall, J.L.

Physiologia Plantarum 59(4): 627-634

1983


ISSN/ISBN: 0031-9317
DOI: 10.1111/j.1399-3054.1983.tb06291.x
Accession: 006287084

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Abstract
Cell wall synthesis was studied by determining the incorporation of [14C]glucose into epidermal and cortical cell walls of etiolated P. sativum L. cv. Alaska stem segments. Walls were fractionated into the matrix and cellulose components, and incorporation into these components assessed in terms of the total uptake of label into that tissue. When segments were allowed to elongate, the stimulation of total glucose uptake by IAA and fusicoccin (FC) was greater than their stimulation of incorporation. IAA and FC thus did not stimulate precursor incorporation in elongating segments. When elongation was inhibited by Ca, however, IAA and FC significantly promoted wall synthesis in the cortex and vascular tissue (which shows almost no growth or acidification response to auxin). In these tissues incorporation into matrix and cellulose was promoted approximately equally. In the epidermis (thought to be the tissue responsive to auxin in the control of growth), FC promoted a significant increase in wall synthesis, although less than that in the cortex, while there was some evidence of a similar promotion by IAA. Both IAA and FC had a greater effect on incorporation into the matrix component of the wall than into cellulose. FC apparently caused a substantial promotion of cell wall synthesis which was not due solely to elongation, and that the inner nongrowth responsive cortical tissues can respond to IAA. A comparison of the effects of IAA and FC on the different components of the wall suggests that the response in the epidermis differs from that in the other tissues.

Regulation of cell wall synthesis by auxin and fusicoccin in different tissues of pea pisum sativum cultivar alaska stem segments