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Regulation of gene expression during aerobic germination of Mucor racemosus sporangiospores

, : Regulation of gene expression during aerobic germination of Mucor racemosus sporangiospores. Journal of General Microbiology 133(1): 141-148

The pool of mRNA stored in dormant sporangiospores of Mucor racemosus and expressed during early germination in air has been investigated. Total RNA was extracted from dormant and germinating spores and translated in a cell-free rabbit reticulocyte system containing L-[35S]methionine. Isotopically labelled in vitro translation products were analysed by PAGE and autoradiography and were compared with labelled proteins synthesized in vivo at the same stages of development. This comparison revealed several significant findings about the fates of individual mRNA populations as templates in translation: (i) a pool of mRNA, presumably represented entirely or in part by a recoverable polyadenylated RNA fraction, can be extracted from dormant spores in a translatable form; (ii) most of the differential gene expression displayed at the level of protein synthesis during germination results from concomitant changes in functional mRNA levels; (iii) some of the stored mRNA species may be activated and others inactivated by post-transcriptional processing mechanisms; and (iv) a small population of gene products may be regulated at the level of selective translation of pre-existing messages.

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Accession: 006288001

PMID: 3655721

DOI: 10.1099/00221287-133-1-141

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Related references

Linz, J.E.; Orlowski, M., 1984: Differential gene expression during aerobic germination of Mucor racemosus sporangiospores. Evidence is provided suggesting that several modes of differential gene expression operate concomitantly during the first 60 min of germination of Mucor racemosus sporangiospores under air. Protein synthesis was initiated immediately upon exposure...

Linz J.E.; Orlowski M., 1991: Differential gene expression and evidence of selective translation during anaerobic germination of mucor racemosus sporangiospores. Anaerobically-germinated sporangiospores of Mucor racemosus develop into yeasts, whereas aerobically-germinated sporangiospores become hyphae. Anaerobic germination was found to have the following traits in common with the previously characterized...

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