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Regulation of gene expression in Dictyostelium discoideum cells exposed to immobilized carbohydrates


, : Regulation of gene expression in Dictyostelium discoideum cells exposed to immobilized carbohydrates. Embo Journal 3(1): 193-200

When amoebae of Dictyostelium discoideum develop on gels of polyacrylamide that are derivatized with glucosides, they become capable of aggregation at the same time as cells not exposed to glucosides. However, the aggregation centers and streams of adherent cells formed on immobilized glucosides suddenly disintegrate. The cells repeatedly re-aggregate, but never form tight aggregates as they do on other substrata. Tight aggregates formed in the absence of glucosides disperse after their transfer to glucoside gels, and the cells undergo aggregation-disaggregation cycles. The formation of tight aggregates is correlated with the expression of specific post-aggregative poly(A) RNAs. These RNAs are not expressed in cells developing on glucoside gels, and the dispersal of tight aggregates on such gels is accompanied by the almost complete loss of these RNAs. A developmentally regulated membrane glycoprotein called contact site A, which is a marker of aggregation-competent cells, is normally expressed on glucoside gels. Cyclic AMP is also produced, indicating that the strong increase of adenylate cyclase activity during the preaggregation phase is not affected. In conclusion, cell contact with immobilized glucosides specifically inhibits postaggregative gene expression and arrests development at the aggregation stage.

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Accession: 006288006

PMID: 16453493

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Related references

Bozzaro S., 1984: Effects of dictyostelium discoideum cell binding to immobilized carbohydrates on cell development and gene expression. Biology of the Cell (Paris) 51(2): 46A

Bozzaro, S.; Roseman, S., 1983: Adhesion of Dictyostelium discoideum cells to carbohydrates immobilized in polyacrylamide gels: effect of D-glucoside derivatives on development. The Journal of Biological Chemistry 258: 890-9

Bozzaro, S.; Roseman, S., 1983: Adhesion of Dictyostelium discoideum cells to carbohydrates immobilized in polyacrylamide gels. II. Effect of D-glucoside derivatives on development. In an accompanying report (Bozzaro, S., and Roseman, S. (1983) J. Biol. Chem. 258, 13882-13889), evidence is presented that the slime mold Dictyostelium discoideum contains three cell surface receptors specific for D-glucose, D-mannose, and N-acet...

Bozzaro, S.; Roseman, S., 1983: Adhesion of dictyostelium discoideum cells to carbohydrates immobilized in poly acrylamide gels 2. effect of d glucoside derivatives on development. D. discoideum contains 3 cell surface receptors specific for D-glucose, D-mannose and N-acetyl-D-glucosamine, respectively. The synthetic probes used for these studies consisted of the sugars covalently linked to polyacrylamide gels. Starved cells...

Bozzaro, S.; Roseman, S., 1983: Adhesion of Dictyostelium discoideum cells to carbohydrates immobilized in polyacrylamide gels: evidence for three sugar-specific cell surface receptors. The Journal of Biological Chemistry 258: 882-9

Bozzaro, S.R.seman, S., 1983: Adhesion of Dictyostelium discoideum cells to carbohydrates immobilized in polyacrylamide gels. II. Effect of D-glucoside derivatives on development Slime molds. Journal of biological chemistry, 258(22): 13890-13897

Bozzaro, S.; Roseman, S., 1983: Adhesion of Dictyostelium discoideum cells to carbohydrates immobilized in polyacrylamide gels 1 Evidence for three sugar-specific cell surface receptors. Dictyostelium discoideum cells appear to be able to recognize particular carbohydrate prosthetic groups at different stages in their life cycle. We therefore used our previously developed model system (consisting of polyacrylamide gels containing...

Bozzaro, S.; Roseman, S., 1983: Adhesion of Dictyostelium discoideum cells to carbohydrates immobilized in polyacrylamide gels. I. Evidence for three sugar-specific cell surface receptors. Dictyostelium discoideum cells appear to be able to recognize particular carbohydrate prosthetic groups at different stages in their life cycle. We therefore used our previously developed model system (consisting of polyacrylamide gels containing...

Bozzaro, S.; Roseman, S., 1983: Adhesion of dictyostelium discoideum cells to carbohydrates immobilized in poly acrylamide gels 1. evidence for 3 sugar specific cell surface receptors. D. discoideum cells appear to be able to recognize particular carbohydrate prosthetic groups at different stages in their life cycle. A previously developed model system (consisting of polyacrylamide gels containing putative ligands covalently lin...

Bozzaro, S.R.seman, S., 1983: Adhesion of Dictyostelium discoideum cells to carbohydrates immobilized in polyacrylamide gels. I. Evidence for three sugar-specific cell surface receptors Slime molds. Journal of biological chemistry, 258(22): 13882-13889