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Regulation of gene expression of ribulose bisphosphate carboxylase in greening pea leaves


, : Regulation of gene expression of ribulose bisphosphate carboxylase in greening pea leaves. Plant Molecular Biology 8(5): 375-382

Ribulose bisphosphate carboxylase/oxygenase (RuBisCO) is composed of two subunits, the chloroplast-coded large subunit (LS) and the nuclear-coded small subunit (SS). The effects of different light doses on the levels of the large subunit gene (rbcL), the rbcL and the rbcS mRNAs, and the rate of synthesis of RuBisCO were followed during greening. The rbcL gene dosage changed in response to light whereas the level of the rbcL mRNA changed independently of the gene dosage. This suggests that the expression of the rbcL gene is transcriptionally regulated and that the change in gene dosage only partially contributes to the increase in the mRNA. It appeared that the RuBisCO synthesis rate was proportional to the rbcS mRNA level rather than rbcL mRNA level. These results, taken together with the earlier observations of many researchers, suggest that RuBisCO synthesis in greening pea leaves is controlled primarily at the level of transcription of both genes and is fine-tuned at the post-transcriptional level in chloroplasts, so that the amount of LS is almost stoichiometric to that of SS.

Accession: 006288014

PMID: 24301259

DOI: 10.1007/BF00015815

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Related references

Bennett, J.; Jenkins, G.I.; Hartley, M.R., 1984: Differential regulation of the accumulation of the light-harvesting chlorophyll a/b complex and ribulose bisphosphate carboxylase/oxygenase in greening pea leaves. The photoregulation of chloroplast development in pea leaves has been studied by reference to three polypeptides and their mRNAs. The polypeptides were the large subunit (LSU) and the small subunit (SSU) of ribulose 1,5-bisphosphate carboxylase/ox...

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