EurekaMag.com logo
+ Site Statistics
References:
48,253,228
Abstracts:
22,798,842
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Regulation of genes for glycolytic enzymes in cultured rat hepatoma cell lines


, : Regulation of genes for glycolytic enzymes in cultured rat hepatoma cell lines. European Journal of Biochemistry 169(2): 237-244

We examined the control by hormones and culture conditions of the expression of pyruvate kinase L, aldolase B, and a liver-specific 5.4-kb mRNA species [Pichard, A. L. et al. (1985) Biochem. J. 226, 637.sbd.644] in three rat hepatoma cell lines, MH1C1, Fao and Faza. The expression level of these markers ranges from 2% (for pyruvate kinase L mRNA) to 10.sbd.12% (for 5.4-kb mRNA species) of the glucose-induced mRNA values found in rat liver. The mRNAs of the three liver-specific genes strongly decrease after treatment of the hepatoma cells with cyclic 8-bromo-AMP, cyclic dibutyryl-AMP or forskolin, pyruvate kinase L mRNA being the most sensitive to this inhibiting effect. In contrast, the concentration of pyruvate kinase L mRNA nuclear precursors is not modified by the cyclic AMP analogues, indicating that these agents do not act at the transcriptional level but, instead, probably destabilize the transcripts. Glucose or fructose does not modify the expression of these three marker genes in any of the studied cell lines. Insulin is inefficient in modifying concentrations of the mRNAs for pyruvate kinase L and aldolase B, alone or in the presence of carbohydrates. In contrast, it stimulates about fivefold the expression of the 5.4-kb mRNA species in the MH1C1 cell line; this stimulation is carbohydrate-independent. The hepatoma cell lines mimic, therefore, the effect of cyclic AMP on the inhibition in vivo of the expression of genes encoding glycolytic or lipogenic enzymes [Vaulont, S. et al. (1984) Biochem. Biophys. Res. Commun. 125, 135.sbd.147]. In contrast, the effect of carbohydrates [Munnich, A et al. (1984) J. Biol. Chem. 259, 10228.sbd.10231] is undetectable. The insulin sensitivity of the liver-specific genes is conserved for the 5.4-kb mRNA species only, especially in the MH1C1 cell line, but not for the other investigated mRNAs, which seems to reflect a fundamental difference in the in vivo effect of insulin on these genes. Finally, S1 nuclease mapping of the start-site of pyruvate kinase L gene transcription shows that the normal site used in vivo is also used in the Fao and Faza lines while, in the MH1C1 line, it coexists with multiple aberrant upstream initiation sites.

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 006288021

PMID: 3691493

DOI: 10.1111/j.1432-1033.1987.tb13603.x

PDF Full Text: Regulation of genes for glycolytic enzymes in cultured rat hepatoma cell lines


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Yanaka, M.; Enari, H.; Dewa, T.; Yamazaki, T., 1997: Benzimidazolesulfonamide derivatives and pharmaceutical composition. Official Gazette of the United States Patent & Trademark Office Patents 1199(2): 1158, June 10

Blanco J.C.; Reig S.; D.L.C.esta L., 1992: Distribution status and conservation problems of the wolf canis lupus in spain. The distribution, status and conservation problems of the wolf Canis lupus in Spain were studied in 1987 and 1988. Wolves regularly occurred over 100,000 km2, mainly in the northwest quarter of the country, with an estimated 294 breeding pairs, i....

Dao, T.; Holan, V.; Minowada, J., 1993: Multiple and heterogeneous patterns of cytokine production in 18 leukemia and in vitro transformed mature T cell line reflect the individuality of human leukemias. The relationship between cytokine production patterns and immunophenotype marker profiles was studied in a panel of 18 T cell lines originating from various types of hematological malignancies and from human T cell lymphotropic virus-I (HTLV-I)-tr...

Räßler, F.; Voigt, U.; Metz, S.; Elsner, P.; Schliemann, S., 2016: Fast-growing tumor of the eyelid. Journal der Deutschen Dermatologischen Gesellschaft 14(1): 81-84

MacGregor J. P., 1971: Preliminary Geological and Foundation Investigations for a Nuclear Power Station at Jervis Bay, N.S.W. Rock testing, seismic studies, seismicity, sandstone, settlements, Australia.

Otte, M.; Thurmayr, G.R.; Dageforde, J.; Thurmayr, R.; Forell, M.M., 1985: Pancreatic secretion in endemic sprue. Pancreatic function was estimated by the secretin-pancreozymin test before the use of gluten-free diet in 22 patients with endemic (coeliac) sprue. Water and bicarbonate secretion were within normal limits, with a trend to high-normal values. Rema...

Pollard, M.; Beisson, F.; Li, Y.; Ohlrogge, J.B., 2008: Building lipid barriers: biosynthesis of cutin and suberin. Cutin and suberin are the polymer matrices for lipophilic cell wall barriers. These barriers control the fluxes of gases, water and solutes, and also play roles in protecting plants from biotic and abiotic stresses and in controlling plant morphol...

Stringer, J.; Mackereth, P.A., 2003: Return to TRI: Winston Churchill Fellow and Salford University Scholar travel to Miami and the Keys. During May and June 2003, the 2 authors visited the internationally renowned Touch Research Institute based at the University of Miami and Jackson Memorial Hospital. For one of the authors, Peter Mackereth, this was a return visit. Both authors ar...

Gentry, D.R.bert; Greenwood, R.C.aire; Lawlor, E.J.ne, 2000: Tryptophanyl tRNA synthetase, trpS, of Streptococcus pneumoniae. The invention provides trpS polypeptides and DNA (RNA) encoding trpS polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing trpS polypeptides to screen for antibacterial compound...

Bartram, K., 1989: Bird watching up north. RAOU (Royal Australasian Ornithologists Union) Newsletter, 5 No. 79