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Regulation of glucosamine 6 phosphate deaminase ec synthesis in yeast

, : Regulation of glucosamine 6 phosphate deaminase ec synthesis in yeast. Biochimica et Biophysica Acta 583(1): 28-35

A basal level of glucosamine-6-phosphate deaminase [EC] is detected in yeast cells grown on glucose. However, a burst of enzyme production occurs in the presence of N-acetylglucosamine in pathogenic Candida albicans and non-pathogenic Saccharomyces cerevisiae. The enzyme synthesis stops and its concentration in the cells declines rapidly as soon as N-acetylglucosamine is removed from the medium. Experiments with RNA- and protein-synthesis inhibitors indicate that the appearance of new enzyme activity is dependent on concomitant new protein synthesis and the inducer operates at a transcriptional level. However, inhibition of DNA synthesis either by hydroxyurea or by mitomycin-C does not impair the synthesis of glucosamine-6-phosphate deaminase.

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