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Regulation of glucose 6 phosphate dehydrogenase ec 1.1.1.49 activity in primary rat hepatocyte cultures


, : Regulation of glucose 6 phosphate dehydrogenase ec 1.1.1.49 activity in primary rat hepatocyte cultures. Biochemical and Biophysical Research Communications 96(2): 748-755

The glucose-6-P dehydrogenase specific activity in rat hepatocytes increases approximately 10-fold when the cells are placed into culture for 3 days. The induction requires insulin with maximum enzyme levels occurring at 10-7 M. Pulse-labeling experiments revealed a 10-fold increase in the enzyme's relative rate of synthesis after only 8 h in culture.

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Winberry, L.; Nakayama, R.; Wolfe, R.; Holten, D., 1980: Regulation of glucose-6-P dehydrogenase activity in primary rat hepatocyte cultures. Biochemical and Biophysical Research Communications 96(2): 748-755

Kelley, D.S.; Kletzien, R.F., 1984: Ethanol modulation of the hormonal and nutritional regulation of glucose 6 phosphate dehydrogenase ec 1.1.1.49 activity in primary cultures or rat hepatocytes. The hormonal and nutritional regulation of glucose 6-phosphate dehydrogenase (G6PDH; EC 1.1.1.49) was studied in primary cultures of rat hepatocytes maintained in a chemically defined medium. Inoculation of hepatocytes from starved rats into prima...

Kelley, D.S.; Kletzien, R.F., 1984: Ethanol modulation of the hormonal and nutritional regulation of glucose 6-phosphate dehydrogenase activity in primary cultures of rat hepatocytes. The hormonal and nutritional regulation of glucose 6-phosphate dehydrogenase (G6PDH; EC 1.1.1.49) was studied in primary cultures of rat hepatocytes maintained in a chemically defined medium. Inoculation of hepatocytes from starved rats into prima...

Molero, C.; Benito, M.; Lorenzo, M., 1994: Glucose-6-phosphate dehydrogenase gene expression in fetal hepatocyte primary cultures under nonproliferative and proliferative conditions. The culture of fetal hepatocytes at high cell density for 64 h in medium supplemented with 5 mM glucose produced an induction of glucose-6-phosphate dehydrogenase (G6PD) mRNA in a time-dependent manner. Insulin and triiodothyronine (T3), separatel...

Molero, C.; Valverde, A.M.; Benito, M.; Lorenzo, M., 1992: Phosphoenolpyruvate carboxykinase and glucose-6-phosphate dehydrogenase expression in fetal hepatocyte primary cultures under proliferative conditions. Fetal hepatocytes cultured for 64 h in the presence of glucagon and dexamethasone maintain a quiescent state, showing a low expression of glucose-6-phosphate dehydrogenase (G6PD) and a high induction of phosphoenolpyruvate carboxykinase (PEPCK). U...

Stumpo, D.J.; Kletzien, R.F., 1984: Regulation of glucose 6 phosphate dehydrogenase ec 1.1.1.49 messenger rna by insulin and the glucocorticoids in primary cultures of rat hepatocytes. The levels of functional mRNA encoding glucose-6-phosphate dehydrogenase (G6PDH) were examined in hepatocytes from fasted and fasted carbohydrate-refed rats, and in hepatocytes inoculated into primary culture. Functional G6PDH mRNA was assessed in...

Stumpo, D.J.; Kletzien, R.F., 1984: Regulation of glucose-6-phosphate dehydrogenase mRNA by insulin and the glucocorticoids in primary cultures of rat hepatocytes. The levels of functional mRNA encoding glucose-6-phosphate dehydrogenase (G6PDH; EC 1.1.1.49) were examined in hepatocytes from fasted and fasted/carbohydrate-refed rats and in hepatocytes inoculated into primary culture. Functional G6PDH mRNA was...

Yoshimoto, K.; Nakamura, T.; Ichihara, A.; Niimi, S., 1983: Hormonal regulation of translatable messenger rna of glucose 6 phosphate dehydrogenase ec 1.1.1.49 in primary cultures of adult rat hepatocytes. Quantity of translatable mRNA of glucose-6-phosphate dehydrogenase (D-glucose-6-phosphate: NADP+ 1-oxidoreductase, EC 1.1.1.49) in primary cultures of adult rat hepatocytes subjected to different hormonal conditions was determined with a reticuloc...

Yoshimoto, K.; Nakamura, T.; Niimi, S.; Ichihara, A., 1983: Hormonal regulation of translatable mRNA of glucose-6-phosphate dehydrogenase in primary cultures of adult rat hepatocytes. The quantity of translatable mRNA of glucose-6-phosphate dehydrogenase (D-glucose-6-phosphate: NADP+ 1-oxidoreductase, EC 1.1.1.49) in primary cultures of adult rat hepatocytes subjected to different hormonal conditions was determined with a retic...

Ohmann, E.; Borriss, R.; Rindt, K.P., 1970: Glucose-6-phosphate dehydrogenase in autotrophic microorganisms. II. Regulation of activity of glucose-6-phosphate dehydrogenase in Euglena gracilis and Rhodopseudomonas spheroides. Zeitschrift für Allgemeine Mikrobiologie 10(1): 37-53