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Regulation of glucose 6 phosphate dehydrogenase ec activity in primary rat hepatocyte cultures

, : Regulation of glucose 6 phosphate dehydrogenase ec activity in primary rat hepatocyte cultures. Biochemical and Biophysical Research Communications 96(2): 748-755

The glucose-6-P dehydrogenase specific activity in rat hepatocytes increases approximately 10-fold when the cells are placed into culture for 3 days. The induction requires insulin with maximum enzyme levels occurring at 10-7 M. Pulse-labeling experiments revealed a 10-fold increase in the enzyme's relative rate of synthesis after only 8 h in culture.

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