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Regulation of glucose transport and metabolism in Thiobacillus novellus

, : Regulation of glucose transport and metabolism in Thiobacillus novellus. Journal of Bacteriology 142(2): 639-644

To investigate the physiological basis of decreased rate of glucose utilization by Thiobacillus novellus in a mixotrophic environment (R. C. Perez and A. Matin, J. Bacteriol. 142:633-638, 1980), its glucose transport system was characterized and the modulation of this system as well as enzymes of glucose metabolism by the growth environment was examined. Uptake of 2-deoxy-d-glucose by cell suspensions was almost abolished by respiratory chain inhibitors, and the sugar accumulated unchanged inside the cells against a concentration gradient: its transport is probably linked to the proton electrochemical gradient. The glucose transport system, as well as several enzymes of glucose metabolism, had a high specific activity in heterotrophic cells, intermediate activity in mixotrophic cells, and low activity in autotrophic cells; thus, they are induced by glucose but repressed by thiosulfate, its metabolites, or both. Thiosulfate and sulfite inhibited the glucose transport system uncompetitively and noncompetitively, respectively (apparent K(i) = 3.1 x 10(-2) M and 3.3 x 10(-7) M, respectively) and also inhibited glucose-6-phosphate dehydrogenase activity. Thus, the rate of glucose utilization in mixotrophic environments decreased because thiosulfate and its metabolites repress as well as inhibit the glucose transport system and enzymes of glucose metabolism. The significance of this and other regulatory phenomena that come into play in such environments is discussed.

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Accession: 006288086

PMID: 7380803

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Related references

Schleiss M.R.; Matin A., 1979: Regulation of glucose transport in thiobacillus novellus. Abstracts of the Annual Meeting of the American Society for Microbiology (79): 175

Barridge, J.K.; Shively, J.M., 1968: Phospho lipids of the thiobacilli thiobacillus thioparus thiobacillus intermedius thiobacillus novellus thiobacillus thiooxidans thiobacillus neapolitanus. Journal of Bacteriology 95(6): 2182-2185

Greenley D.E.; Smith D.W., 1979: A novel pathway of glucose catabolism in thiobacillus novellus. Phosphoketolase (E.C. was found in crude extracts of T. novellus (ATCC 8093) with a specific activity of 0.070 .mu.mol triose formed min-1 (mg protein)-1. The pH optimum, 6.0, temperature optimum, C, and Km, 4.27 mM, are in goo...

Kula T., 1974: Transport of di carboxylic acids in thiobacillus novellus. Abstracts of the Annual Meeting of the American Society for Microbiology 74: 191

Aleem, M.I., 1965: Thiosulfate Oxidation and Electron Transport in Thiobacillus novellus. Aleem, M. I. H. (Research Institute for Advanced Studies, Baltimore, Md.). Thiosulfate oxidation and electron transport in Thiobacillus novellus. J. Bacteriol. 90:95-101. 1965.-A cell-free soluble enzyme system capable of oxidizing thiosulfate was...

Mccarthy, J.T.; Charles, A.M., 1975: Properties and regulation of ribulose di phosphate carboxylase from thiobacillus novellus. Archives of Microbiology 105(1): 51-59

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Cole, J.S.; Aleem, M.I., 1973: Electron transport-linked compared with proton-induced ATP generation in Thiobacillus novellus. The apparently soluble electron-transport system, that does not sediment when centrifuged at 144,000 x g or 300,000 x g for 3 hr, catalyzes oxidative phosphorylation with an efficiency comparable to that of an intact mitochondrial system. While th...

Cole, J.S.Iii ; Aleem, M.I.H., 1973: Electron transport linked compared with proton induced atp generation in thiobacillus novellus. Proceedings of the National Academy of Sciences of the United States of America 70((12 PART 1)): 3571-3575