EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Regulation of glutamate biosynthesis and release in vitro by low levels of ammonium ions


Brain Research 237(2): 339-350
Regulation of glutamate biosynthesis and release in vitro by low levels of ammonium ions
The content and release of endogenous amino acids from isolated rat hippocampal slices were measured. The tissue was perfused with control media and pulsed with high K media to induce synaptic release. Pathophysiological concentrations of NH4+ (3-5 mM) were added to the control medium for 60 min prior to the induced release. Amino acids belonging to the putative transmitter group were released extensively during K perfusion and, except for glutamate, even after NH4+ perfusion. The spontaneous secretion of glutamate increased slowly after the addition of NH3. The incorporation of 14C from radiolabeled glucose and acetate into the amino acid fraction was studied in the presence of NH3-containing media. Glucose was utilized to a moderately increasing extent, but acetate-derived radioactivity was strikingly decreased in the amino acid fraction during NH3 perfusion. The decreased acetate incorporation into amino acids was mainly due to an inhibition by NH3 of the accumulation of acetate by the CNS tissue.

Accession: 006288096

PMID: 6123369

DOI: 10.1016/0006-8993(82)90446-2

Download PDF Full Text: Regulation of glutamate biosynthesis and release in vitro by low levels of ammonium ions



Related references

Regulation of glutamate biosynthesis and release by pathophysiological levels of ammonium ions. Advances in Biochemical Psychopharmacology 27: 115-126, 1981

Dual regulation of Ca2+-dependent glutamate release from astrocytes: vesicular glutamate transporters and cytosolic glutamate levels. Glia 57(12): 1296-1305, 2009

Regulation of l glutamate biosynthesis by copper ions. Amino Acid & Nucleic Acid 27: 33-53, 1973

Role of copper ions in the regulation of l glutamate biosynthesis. Agricultural & Biological Chemistry 37(8): 1837-1847, 1973

Requirement of calcium ions and magnesium ions for the in vitro release of follicle stimulating hormone from rat pituitary glands and in its subsequent biosynthesis. Endocrinology 86(4): 761-768, 1970

Regulation of renin release by calcium and ammonium ions in normal man. Journal of Clinical Endocrinology and Metabolism 43(6): 1343-1350, 1976

Effects of ammonium ions on spontaneous action potentials and on contents of sodium potassium ammonium and chloride ions in brain in vitro. Journal of Neurochemistry 30(1): 131-144, 1978

Control of glutaminase activity in rat brain cortex in vitro: influence of glutamate, phosphate, ammonium, calcium and hydrogen ions. Brain Research 208(2): 363-377, 1981

Control of glutaminase ec 3.5.1.2 activity in rat brain cortex in vitro influence of glutamate phosphate ammonium calcium and hydrogen ions. Brain Research 208(2): 363-378, 1981

Magnesium phosphates stimulate microbial conversion of glycine to l serine by release from regulation by ammonium ions. Journal of Fermentation Technology 59(6): 447-456, 1981