+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Regulation of glutamate biosynthesis and release in vitro by low levels of ammonium ions

, : Regulation of glutamate biosynthesis and release in vitro by low levels of ammonium ions. Brain Research 237(2): 339-350

The content and release of endogenous amino acids from isolated rat hippocampal slices were measured. The tissue was perfused with control media and pulsed with high K media to induce synaptic release. Pathophysiological concentrations of NH4+ (3-5 mM) were added to the control medium for 60 min prior to the induced release. Amino acids belonging to the putative transmitter group were released extensively during K perfusion and, except for glutamate, even after NH4+ perfusion. The spontaneous secretion of glutamate increased slowly after the addition of NH3. The incorporation of 14C from radiolabeled glucose and acetate into the amino acid fraction was studied in the presence of NH3-containing media. Glucose was utilized to a moderately increasing extent, but acetate-derived radioactivity was strikingly decreased in the amino acid fraction during NH3 perfusion. The decreased acetate incorporation into amino acids was mainly due to an inhibition by NH3 of the accumulation of acetate by the CNS tissue.

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 006288096

PMID: 6123369

DOI: 10.1016/0006-8993(82)90446-2

PDF Full Text: Regulation of glutamate biosynthesis and release in vitro by low levels of ammonium ions

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Ma-Chen-Ming; Chen-Yu; Xie-Ji-Yan; Wan-Feng, 2008: Quality of life five years after coronary artery bypass grafting. Objective The quality of life (QOL) 5 years after coronary artery bypass grafting (CABG) was evaluated by NHP, SAQ and DASI questionnaires. Methods NHP, SAQ and DASI questionnaires were mailed to 287 patients who received CABG in our department b...

Howell, T.H., 1943: Disease and Prognosis in Old Age. Postgraduate Medical Journal 19(216): 258-260

Morrison S.C.; Myburgh L.D.; Fourie A.T.J.; Terblanche L., 1991: The flow volume loop maximum voluntary ventilation and exercise capacity in divers under hyperbaric conditions. American Review of Respiratory Disease 143(4 PART 2): A345

Toukan, A.U., 1987: Hepatitis B virus infection in urban residents of Jordan with particular reference to socioeconomic factors. Tropical Gastroenterology 8(3): 161-166

Bouvier, M.; Bonvoisin, B.; Lejeune, E.; Sidot, C.; Touraine, J.L.; Touraine, F., 1983: Treatment of rheumatoid arthritis with isoprinosine. Personal experience. Twenty patients with classical or proved rheumatoid arthritis were treated with Isoprinosine. 13 patients received a dose of 25 mg/kg/day and 7 received a dose of 50 mg/kg/day, continuously for 2 months and then discontinuously, 5 days every fortn...

Peterson, K.E.; Fox, M.Kay., 2007: Addressing the epidemic of childhood obesity through school-based interventions: what has been done and where do we go from here?. Schools are ideal settings for implementing multi-component programs to prevent and control childhood obesity. Thoughtful improvements to proven strategies, coupled with careful evaluation, can contribute to accumulation of evidence needed to desi...

Matsumoto, M.; Shishido, A.; Ueda, K.; Tagaya, I.; Ishii, K., 1970: Symposium. Clinical and basic studies on viral exanthema. Kansenshogaku Zasshi. Journal of the Japanese Association for Infectious Diseases 44(9): 479-482

Logemann, J.A.; Smith, C.H.; Pauloski, B.R.a; Rademaker, A.W.; Lazarus, C.L.; Colangelo, L.A.; Mittal, B.; Maccracken, E.; Gaziano, J.; Stachowiak, L.; Newman, L.A., 2001: Effects of xerostomia on perception and performance of swallow function. Background: Head and neck cancer treatment with high-dose chemoradiation may cause xerostomia and affect the patient's perception of swallowing ability. Method: Whole saliva production was measured in 36 patients with advanced-stage cancer of...

Martynenko A.M., 1971: The nature of pain in pre cancer and cancer of the tongue of diverse stages. Stomatologiya (Moscow) 50(2): 90

Brandes, W.; Heitefuss, R., 1971: Side effects of herbicides on E. graminis and C. herpotrichoides on Wheat. I. Plant infection under the influence of herbicides. II. Physiological and biochemical factors related to the altered plant infection. Triazine and urea-type herbicides increased E. graminis on spring and winter wheat in field experiments. A significant preliminary reduction in both E. graminis and C. herpotrichoides occurred in glasshouse tests with these herbicides; later milde...