Regulation of glutamate dehydrogenase ec 1.4.1.2 activity by amino acids in maize seedlings zea mays cultivar ganga safed 2

Singh, R.P.; Srivastava, H.S.

Physiologia Plantarum 57(4): 549-554

1983


ISSN/ISBN: 0031-9317
DOI: 10.1111/j.1399-3054.1983.tb02784.x
Accession: 006288099

Download citation:  
Text
  |  
BibTeX
  |  
RIS

Article/Abstract emailed within 0-6 h
Payments are secure & encrypted
Powered by Stripe
Powered by PayPal

Abstract
Root or secondary leaf segments from maize (Z. mays L. cv. Ganga safed-2) seedlings were incubated with 9-amino acids and 2 amides separately, each at 5 mM for 24 h, to study their effects on glutamate dehydrogenase (GDH) activity. Most of the compounds tested inhibited the specific activity of NADH-GDH and increased that of NAD+-GDH in the roots in the presence as well as in the absence of ammonium. In the leaves, such effects were recorded only with a few amino acids. Total soluble protein in the root and leaf tissues increased with the supply of most of the amino compounds. The effect of glutamate on enzyme activity and protein was concentration dependent in both tissues. When the enzyme extracts from root or leaf tissues were incubated with some of the amino acids, NADH-GDH declined while NAD+-GDH increased in most cases. The inhibition of NADH-GDH increased with increasing concentratin of cysteine from 1 to 5 mM. Evidently, most of the amino acids regulated GDH activity, possibly through some physicochemical modulation of the enzyme molecule.

Regulation of glutamate dehydrogenase ec 1.4.1.2 activity by amino acids in maize seedlings zea mays cultivar ganga safed 2