+ Translate

Regulation of glutamine synthetase ec activity by adenylylation in the gram positive bacterium streptomyces cattleya

, : Regulation of glutamine synthetase ec activity by adenylylation in the gram positive bacterium streptomyces cattleya. Proceedings of the National Academy of Sciences of the United States of America 78(1): 229-233

The enzymatic activity of glutamine synthetase [GS; L-glutamate:ammonia ligase (ADP-forming), EC] from the gram-positive bacterium S. cattleya is regulated by covalent modification. In whole cells containing high levels of GS the addition of ammonium chloride leads to a rapid decline in GS activity. Crude extracts prepared from such ammonia-shocked cells had very low levels of GS activity as measured by biosynthetic and .gamma.-glutamyltransferase assays. Incubation of the crude extracts with snake venom phosphodiesterase restored GS activity. In cell extracts, GS was also inactivated by an ATP- and glutamine-dependent reaction. Radioactive labeling studies demonstrated the incorporation of an AMP moiety into GS protein upon modification. The results suggest a covalent modification of GS in a gram-positive bacterium. This modification appears to be adenylylation of the GS subunit similar to that found in the gram-negative bacteria.

(PDF 0-2 workdays service)

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 006288127

PDF Full Text: Submit

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Kyaw, T.; Curry, A.; Edwards-Jones, V.; Craske, J.; Mandal, B.K., 1998: The prevalence of Enterocytozoon bieneusi in acquired immunodeficiency syndrome (AIDS) patients from the north west of England: 1992-1995. Microsporidia are increasingly being recognised as important enteric pathogens in patients with advanced human immunodeficiency virus (HIV) disease, i.e. acquired immunodeficiency syndrome (AIDS). The aims of this study were to investigate the fre...

Collins, M. L., 1998: Target and background capture methods and apparatus for affinity assays. Official Gazette of the United States Patent & Trademark Office Patents 1212(2): 1765, July 14

Sternberg, J., 1970: Irradiation and radiocontamination during pregnancy. American Journal of Obstetrics and Gynecology 108(3): 490-513

Goodwin N.J., 1969: Colistin sulfate vs sodium colistimethate. Annals of Internal Medicine 70(1): 232-233

Weatherburn, P.; Hickson, F.; Reid, D.; Torres-Rueda, S.; Bourne, A., 2016: Motivations and values associated with combining sex and illicit drugs ('chemsex') among gay men in South London: findings from a qualitative study. There is considerable public health concern about the combining of sex and illicit drugs (chemsex) among gay men. With a view to inform supportive therapeutic and clinical interventions, we sought to examine the motivations for engaging in chemsex...

Barcia D.; Martinez Pardo Y.F., 1978: Parkinsons disease with depression treated with electro convulsive therapy. Archivos de Neurobiologia 41(5): 393-398

Machado-Assefh, C.Renata.; Alvarez, A.Elisabet., 2016: Probing behavior of aposymbiotic green peach aphid (Myzus persicae) on susceptible Solanum tuberosum and resistant Solanum stoloniferum plants. NlmCategory="UNASSIGNED">The green peach aphid, Myzus persicae Sulzer (Hemiptera: Aphididae) is one of the potato important pests; it is the most efficient vector of potato viruses. Myzus persicae harbors the endosymbiotic bacteria Bu...

Roebuck, J. F., 1987: Agricultural problems of weeds on the crop headland. The origins, biology and control of weeds on headlands of arable fields in the UK are discussed with special reference to Alopecurus myosuroides, Avena fatua, Apera spica-venti, Bromus spp., Phalaris spp., Agrostis spp., Elymus repens, Poa trivial...

Libre, P.E.; Harmon, G.K.; Adieh, H., 1995: Intraoperative subconjunctival injection of mitomycin C in trabeculectomy. Investigative Ophthalmology & Visual Science 36(4): S86

Sell P.D., 1981: Lapsana intermedia or lapsana communis ssp intermedia. A brief survey of variation in the genus Lapsana L. in Europe and southwest Asia is given, together with reasons for accepting only 1 sp. and several subspecies. This includes the plants recorded for Britain by Burtt (1950) as L. intermedia Bieb....