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Regulation of glutamine synthetase ec 6.3.1.2 from saccharomyces cerevisiae by repression inactivation and proteolysis






European Journal of Biochemistry 123(3): 611-616

Regulation of glutamine synthetase ec 6.3.1.2 from saccharomyces cerevisiae by repression inactivation and proteolysis

Glutamine synthetase activity is modulated by N repression and by 2 distinct inactivation processes. Addition of glutamine to exponentially grown yeast leads to enzyme inactivation. 50% of glutamine synthetase activity is lost after 30 min (a quarter of the generation time). Removing glutamine from the growth medium results in a rapid recovery of enzyme activity. A regulatory mutation (gdhCR mutation) suppresses this inactivation by glutamine in addition to its depressing effect on enzymes involved in N catabolism. The gdhCR mutation also increases the level of proteinase B in exponentially grown yeast. Inactivation of glutamine synthetase is also observed during N starvation. This inactivation is irreversible and probably consists of a proteolytic degradation. Strains bearing proteinase A, B and C mutations are no longer inactivated under N starvation.


Accession: 006288130



Related references

Legrain, C.; Vissers, S.; Dubois, E.; Legrain, M.; Wiame, J.M., 1982: Regulation of glutamine synthetase from Saccharomyces cerevisiae by repression, inactivation and proteolysis. Glutamine synthetase activity is modulated by nitrogen repression and by two distinct inactivation processes. Addition of glutamine to exponentially grown yeast leads to enzyme inactivation. 50% of glutamine synthetase activity is lost after 30 mi...

Mitchell, A.P.; Magasanik, B., 1984: Biochemical and physiological aspects of glutamine synthetase inactivation in Saccharomyces cerevisiae. Saccharomyces cerevisiae glutamine synthetase is inactivated in vivo by the addition of glutamine or ammonia. Inactivation is characterized by a specific loss of synthetase activity; transferase activity remains stable. Several physiological pertu...

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Dubois, E.L.; Grenson, M., 1974: Absence of involvement of glutamine synthetase and of NAD-linked glutamate dehydrogenase in the nitrogen catabolite repression of arginase and other enzymes in Saccharomyces cerevisiae. Biochemical and Biophysical Research Communications 60(1): 150-157

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Kim K.; Rhee S.G., 1985: Regulation of glutamine synthetase activity in saccharomyces cerevisiae. Federation Proceedings 44(3): 682

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Mecke, D.; Holzer, H., 1966: Repression and inactivation of glutamine synthetase in Escherichia coli by NH4+. Glutamine synthetase (L-glutamate ammonia ligase (ADP), EC 6.3.1.2) is repressed in E. coli when this organism is grown in a medium containing NH4 +. Removal from NH4 + and resuspension in a glutamate-containing medium causes derepression. This de...