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Regulation of glutamine synthetase ec 6.3.1.2 in the blue green alga anabaena flos aquae


, : Regulation of glutamine synthetase ec 6.3.1.2 in the blue green alga anabaena flos aquae. Biochemical and Biophysical Research Communications 96(2): 975-983

Glutamine synthetase (GS) was isolated from log phase cells and purified to a single protein as evidenced by gel electrophoresis. Protamine and ammonium sulfate precipitation and chromatography on DEAE-cellulose and Bio-Gel resulted in 380-fold purification. The enzyme was most sensitive to alanine (85% inhibition at 0.1 mM) but was also inhibited by glycine, arginine and serine. Combinations of inhibitory amino acids or nucleotides (AMP, ADP, ATP) exhibited cumulative inhibition. Cooperative inhibition was noted with CTP and any single nucleotide. Inhibition by CTP alone was uncompetitive with respect to glutamine. The enzyme was also regulated by the energy charge of the cell.

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McMaster, B.J.; Danton, M.S.; Storch, T.A.; Dunham, V.L., 1980: Regulation of glutamine synthetase in the blue-green alga Anabaena flos-aquae. Glutamine synthetase (GS) was isolated from log phase cells and purified to a single protein as evidenced by gel electrophoresis. Protamine and ammonium sulfate precipitation and chromatography on DEAE-cellulose and Bio-Gel resulted in 380-fold pu...

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Carlton, J.R.; Herson, D.S., 1972: The electrophoretic characterization of ribosomes from the blue-green alga Anabaena flos-aquae. Archiv für Mikrobiologie 86(1): 39-48

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Bostwick, C.D.; Brown, L.R.; Tischer, R.G., 1968: Some observations on the sodium and potassium interactions in the blue-green alga Anabaena flos-aquae A-37. The growth of Anabaena flos-aquae A-37 has been shown to be severely limited by the absence of either Na or K from the culture medium. Neither element is capable of replacing the other. The addition of Na to Na-starved cells restores growth while...