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Regulation of glutamine synthetase ec 6.3.1.2 in the blue green alga anabaena flos aquae


Biochemical and Biophysical Research Communications 96(2): 975-983
Regulation of glutamine synthetase ec 6.3.1.2 in the blue green alga anabaena flos aquae
Glutamine synthetase (GS) was isolated from log phase cells and purified to a single protein as evidenced by gel electrophoresis. Protamine and ammonium sulfate precipitation and chromatography on DEAE-cellulose and Bio-Gel resulted in 380-fold purification. The enzyme was most sensitive to alanine (85% inhibition at 0.1 mM) but was also inhibited by glycine, arginine and serine. Combinations of inhibitory amino acids or nucleotides (AMP, ADP, ATP) exhibited cumulative inhibition. Cooperative inhibition was noted with CTP and any single nucleotide. Inhibition by CTP alone was uncompetitive with respect to glutamine. The enzyme was also regulated by the energy charge of the cell.


Accession: 006288133



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