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Regulation of glycogen metabolism in primary cultures of rat hepatocytes. Restoration of acute effects of glucose in cells from diabetic rats involves protein synthesis


, : Regulation of glycogen metabolism in primary cultures of rat hepatocytes. Restoration of acute effects of glucose in cells from diabetic rats involves protein synthesis. Journal of Biological Chemistry 262(9): 4000-4006

Defective acute regulation of hepatic glycogen synthase by glucose and insulin, caused by severe insulin deficiency, can be corrected in adult rat hepatocytes in primary culture by inclusion of insulin, triiodothyronine, and cortisol in a chemically defined serum-free culture medium over a 3-day period (Miller, T. B., Jr., Garnache, A. K., Cruz, J., McPherson, R. K., and Wolleben, C. (1986) J. Biol. Chem. 261, 785-790). Using primary cultures of hepatocytes isolated from normal and diabetic rats in the same serum-free chemically defined medium, the present study addresses the effects of cycloheximide and actinomycin D on the chronic actions of insulin, triiodothyronine, and cortisol to facilitate the direct effects of glucose on the short-term activation of glycogen synthase. The short-term presence (1 h) of the protein synthesis blockers had no effect on acute activation of glycogen synthase by glucose in primary hepatocyte cultures from normal rats. Normal cells maintained in the presence of cycloheximide or actinomycin D for 2 and 3 days exhibited unimpaired responsiveness to glucose activation of synthase. The protein synthesis inhibitors were effective at blocking the restoration of glucose activation of synthase in diabetic cells in media which restored the activation in their absence. Restoration of glycogen synthase phosphatase activity by insulin, triiodothyronine, and cortisol in primary cultures of diabetic hepatocytes was also blocked by cycloheximide or actinomycin D. These data clearly demonstrate that restoration of acute glycogen synthase activation by glucose and restoration of glycogen synthase phosphatase activity in primary cultures of hepatocytes from adult diabetic rats are dependent upon the synthesis of new protein.

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Accession: 006288167

PMID: 3104335

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Ray, Gh, 1979: Plums for the backyard garden. Minnesota horticulturist 107(8): 224-226

Smith G.L.F., 1992: Diagnosis of periodontal disease activity by detection of key microbial antigens. Journal of Dental Research 71(SPEC ISSUE): 746

Slabospitskaya A.T.; Reznik S.R., 1980: Effect of preparation 6 on digestive tract micro flora. Microorganisms isolated from the digestive tract of patients were studied as affected by preparation 6. The preparation produces an antibacterial effect on Bacillus subtilis and Candida, to a lesser extent on staphylococci, and has no influence on...

Cairns, John Jr, 1992: Monitoring for global change. (see Book Title) [Author] Cairns, J Jr , B R Niederlehner and D R Orvos (Ed ) Advances in Modern Environmental Toxicology, Vol XX Predicting ecosystem risk xx+347p Princeton Scientific Publishing Co , Inc : Princeton, New Jersey, USA Illus ISBN 0-911131-27-2 : 63-75

Shields, Janiel M.; Christy, Robert J.; Yang, Vincent W., 1996: Identification and characterization of a gene encoding a gut-enriched Kruppel-like factor expressed during growth arrest. The Journal of Biological Chemistry 271: 009-17

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Ogami K.; Hadzopoulou Cladaras M.; Cladaras C.; Zannis V.I., 1989: Promoter elements required for transcription of the human apociii gene. Circulation 80(4 SUPPL 2): II263

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Rebers, P. A.; Barker, S. A.; Heidelberger, M.; Dische, Z.; Evans, E. E., 1958: Precipitation of the specific polysaccharide of Cryptococcus neoformans A by types n and XIV of antipneumococcal sera. It is reported in this joint contribution from the Universities of Rutgers, Columbia, and Alabama that type-specific polysaccharide of C. neoformans A, known to contain xylose, galactose, mannose, and glucuronic acid, is a mixture of at least 2 po...

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