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Regulation of glycolysis and purine nucleotide metabolism in the enzymatic phosphorylation system of mucor aromaticus

Weishengwu Xuebao 20(2): 157-165

Regulation of glycolysis and purine nucleotide metabolism in the enzymatic phosphorylation system of mucor aromaticus

The character of glycolysis, the effects of glucose and phosphate on glycolysis and the regulation of purine nucleotide metabolism by M. aromaticus were studied. Although M. aromaticus possess strong fermenting power, the phosphorylation of glucose in M. aromaticus is easily effected by the storage temperature of the mycelia. That may be the key factor affecting glycolysis and phoshorylation of AMP of M. aromaticus. In the reaction system of enzymatic phosphorylation of M. aromaticus, not only the phosphorylation efficiency of AMP, but also the accumulation rate of ATP were affected by the concentration of glucose and the molar ratio of glucose:phosphate (G/P), played an important role in regulating the activation of the enzyme system of glycolysis and purine nucleotide metabolism. The degradation of AMP was inhibited as G/P < 1. The deamination of AMP brought forth the IMP and the decomposition of latter brought forth Ino and/or Hx as G/P .gtoreq. 1. Under certain concentration of glucose, the high concentration of phosphate could inhibit deamination of AMP and decomposition of IMP, and promote phosphorylation of AMP. It seems that under certain concentration of phosphate, the inhibition effect of the phosphate on the activity of AMP deaminase and Ino hydrolase can be eliminated when G/P .gtoreq. 1, AMP was deaminated to form IMP and the latter was further decomposed to Hx. Therefore, G/P play an important regulative role for metabolic pathway of purine nucleotide of M. aromaticus.

Accession: 006288203

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