EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Regulation of glycolysis fructolysis in buffalo bubalus bubalis spermatozoa


Journal of Reproduction & Fertility 64(1): 145-150
Regulation of glycolysis fructolysis in buffalo bubalus bubalis spermatozoa
Assay of maximal activities of 11 glycolytic enzymes in cell-free buffalo sperm extracts showed that hexokinase, phosphofructokinase and glyceraldehyde-3-phosphate dehydrogenase had the lowest activities, suggesting regulation of fructolysis at steps catalyzed by these enzymes. The ratios of glyceraldehyde-3-phosphate dehydrogenase/phosphofructokinase (0.67) and phosphoglycerate kinase/phosphofructokinase (4.60) are typical of cells exhibiting high Pasteur effect (50% for ejaculated buffalo spermatozoa). The regulatory nature of phosphofructokinase was shown through its modulation by ATP, AMP and Pi. The determination of fructolytic intermediates and cofactors and calculation of mass action ratios for each enzymic step revealed that hexokinase, phosphofructokinase, fructose-biphosphate aldolase, glyceraldehyde-3-phosphate dehydrogenase and pyruvate kinase catalyzed reactions far removed from the equilibrium. A regulatory role by glyceraldehyde-3-phosphate dehydrogenase appeared to be most likely because triosephosphates and inorganic Pi accumulated more under anaerobic than under aerobic conditions.


Accession: 006288205



Related references

Effects of steroids on the fructolysis index of buffalo bubalus bubalis spermatozoa. Journal of Steroid Biochemistry 9(9): 824, 1978

Effects of steroids on fructolysis index of buffalo bubalus bubalis spermatozoa. Indian Journal of Animal Sciences 49(10): 782-786, 1979

REgulation of glycolysis/fructolysis in buffalo spermatozoa. Journal of Reproduction and Fertility 64(1): 145-150, 1982

Regulation of fructolysis glycolysis in ejaculated buffalo spermatozoa. Indian Journal Of Biochemistry & Biophysics: 64, 1981

On the leakage of acrosomal hyaluronidase from the spermatozoa of the buffalo (Bubalus bubalis). Reproduction, Nutrition, Developpement 20(3a): 593-599, 1980

Morphometric maturational changes of epididymal spermatozoa in the buffalo bubalus bubalis. Zuchthygiene (Berlin) 18(2): 58-62, 1983

Complex staining of water buffalo (Bubalus bubalis) spermatozoa. Bubalus Bubalis 9(2): 55-60, 2003

The leakage of acrosomal hyaluronidase from the spermatozoa of the buffalo bubalus bubalis. Reproduction Nutrition Developpement 20(3 PART A): 593-599, 1980

Spectroscopic and microscopic studies of buffalo-bull (Bubalus bubalis) spermatozoa. Journal of Submicroscopic Cytology and Pathology 25(1): 37-46, 1993

Glycocalyx of buffalo bubalus bubalis spermatozoa a cytochemical and biochemical study. Indian Journal of Experimental Biology 18(1): 48-50, 1980