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Regulation of glycolysis in rat aorta


, : Regulation of glycolysis in rat aorta. American Journal of Physiology 247(1 Pt 1): C107-C114

Certain factors that might contribute to the regulation of the rate of glycolysis by rat aorta were investigated. Rat aortic rings were incubated with [14C]glucose, and the release of [14C]lactate was determined. There was good agreement between the lactate production estimated by enzymatic assay and by [14C]lactate release, suggesting that almost all the lactate produced under our experimental conditions was derived from exogenous glucose. When the glucose concentration in the medium was 10 mM or higher, the rate of glucose transport did not limit the rate of lactate production. In most cases studies were done both aerobically and anaerobically. In Hanks' Balanced Salt Solution the aerobic rate of lactate production was 18% of the anaerobic rate. The effects on glycolysis of agents that alter ATP generation by mitochondria or ATP splitting by Na+-K+-ATPase or the mitochondrial ATPase was tested. Under aerobic conditions, ouabain (5 mM) caused a 54% decrease in lactate production, and gramicidin (5 .mu.g/ml) caused a 45% increase. Under anaerobic conditions, neither ouabain nor gramicidin affected lactate production. Aerobically dinitrophenol (25 .mu.M) and carboxyatractyloside (0.5 mM) caused substantial increases in lactate production, 72 and 98%, respectively. Under anaerobic conditions the effects of dinitrophenol and carboxyatractyloside were much smaller, with dinitrophenol causing a 15% increase and carboxyatractyloside a 12% decrease in lactate production. Increasing the concentration of phosphate in the incubation medium caused marked increases in lactate production. Both aerobically and anaerobically, shifting from 1.3 to 50 mM phosphate in the incubation medium caused a 3.5-fold increase in lactate production. Increased phosphate had no effect on the rate of lactate oxidation.

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Accession: 006288210

PMID: 6204540

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Otto, G., 1972: Studies on the cause of specific replant disease of fruit trees. III. Experiments to eradicate specific replant disease by soil steaming at different temperatures. Samples of affected soil were steamed at 40 - 100 deg C for 1 h and, after drying, placed in 23-cm clay pots. An apple seedling in the 3-leaf stage was planted in each pot. Temperatures up to 50 deg did not alter the growth depression induced by r...

Li, C.; Li, J.Fang.; Cai, Q.; Qiu, Q.Qing.; Yan, M.; Liu, B.Ya.; Zhu, Z.Gang., 2014: miRNA-199a-3p in plasma as a potential diagnostic biomarker for gastric cancer. MicroRNA (miRNA) has been shown the potential of cancer diagnosis. We investigated whether plasma miRNA expression could discriminate between patients with and without gastric cancer. This study was divided into three steps: (1) miRNA microarray p...

O'donnell J.M., 1988: Tolerance to the behavioral effects of the centrally active beta adrenergic agonist clenbuterol. Society for Neuroscience Abstracts 14(1): 305

Paul, A. K.; Roy, P.; Dasgupta, B., 1983: A case of cannibalism in Chrysomya rufifacies (Macquart) (Diptera: Calliphoridae). Dipterous larvae that were observed feeding on adult females of Chrysomya megacephala caught in a cage-trap at Jalpaiguri in West Bengal, India, were identified as C. rufifacies; some of these larvae were removed and reared on decaying fish (the n...

Schoeman, David, S.; Nel, Ronel; Soares, Alexandre-Goulart, 2008: Measuring species richness on sandy beach transects extrapolative estimators and their implications for sampling effort. Species richness is a measure that is fundamental to many studies in ecology, and it is particularly important on sandy beaches, where it underlies patterns described by the broadly accepted swash exclusion hypothesis. However, its estimation in...

Sloan, D. R.; Harms, R. H.; Smith, W. G., 1995: Influence of dietary calcium level, corn oil and total sulfur amino acid level on egg size. Poultry Science 74(SUPPL 1): 218

Welbank, P. J., 1962: The effects of competition with Agropyron repens and of nitrogen- and water-supply on the nitrogen content of Impatiens parviflora. The nitrogen contents of plants of Impatiens parviflora grown in pots with and without root competition from Agropyron repens, and with two levels of nitrogen- and water-supply, were determined. The nitrogen percentage and the nitrogen content wer...

Narayan, M.; Welker, E.; Scheraga, H.A., 2003: Characterizing the unstructured intermediates in oxidative folding. A recently developed method is used here to characterize some of the folding intermediates, and the oxidative folding processes, of RNase A. This method is based on the ability of trans-[Pt(en)(2)Cl(2)](2+) to oxidize cysteine residues to form dis...

Middleditch, John, 2012: Pulsar-Driven Jets in Supernovae, Gamma-Ray Bursts, and the Universe. The bipolarity of Supernova 1987A can be understood through its very early light curve from the CTIO 0.4?m telescope and IUE FES and following speckle observations of the Mystery Spot . These indicate a beam/jet of light/particles, with initial co...

Rogan E.; Bodell W.; Devanesan P.; Cavalieri E., 1989: Characterization of benzo a pyrene bp dna adducts formed in vitro and in mouse skin in vivo. Proceedings of the American Association for Cancer Research Annual Meeting 30: 117