EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Regulation of glycoprotein D synthesis: does alpha 4, the major regulatory protein of herpes simplex virus 1, regulate late genes both positively and negatively?






Journal of Virology 62(1): 148-158

Regulation of glycoprotein D synthesis: does alpha 4, the major regulatory protein of herpes simplex virus 1, regulate late genes both positively and negatively?

Earlier studies have described the .alpha.4/c113 baby hamster kidney cell line which constitutively expresses the .alpha.4 protein, the major regulatory protein of herpes simplex virus 1 (HSV-1). Introduction of the HSV-1 glycoprotein B (gB) gene, regulated as a .gamma.1 gene, into these cells yielded a cell line which constitutively expressed both the .alpha.4 and .gamma.1 gB genes. The expression of the gB gene was dependent on the presence of functional .alpha.4 protein. In this article we report that we introduced into the .alpha.4/c113 and into the parental BHK cells, the HSV-1 BamHI J fragment, which encodes the domains of four genes, including those of glycoproteins D, G, and I (gD, gG, and gI), and most of the coding sequences of the glycoprotein E (gE) gene. In contrast to the earlier studies, we obtained significant constitutive expression of gD (also a .gamma.1 gene) in a cell line (BJ) derived from parental BHK cells, but not in a cell line (.alpha.4/BJ) which expresses functional .alpha.4 protein. RNA homologous to the gD gene was present in significant amounts in the BJ cell line; smaller amounts of this RNA were detected in the .alpha.4/BJ cell line. RNA homologous to gE, presumed to be polyadenylated from signals in the vector sequences, was present in the BJ cells but not in the .alpha.4/BJ cells. The expression of the HSV-1 gD and gE genes was readily induced in the .alpha.4/BJ cells by superinfection with HSV-2. The BJ cell line was, in contrast, resistant to expression of HSV-1 and HSV-2 genes. The BamHI J DNA fragment copy number was approximately 1 per BJ cell genome equivalent and 30 to 50 per .alpha.4/BJ cell genome equivalent. We conclude that (i) the genes specifying gD and gB belong to different viral regulatory gene subsets, (ii) the gD gene is subject to both positive and negative regulation, (iii) both gD and gE mRNAs are subject to translational controls although they may be different, and (iv) the absence of expression of gD in the .alpha.4/BJ cells reflects the expression of the .alpha.4 protein in these cells.


Accession: 006288221

PMID: 2824843



Related references

Sivropoulou, A.; Arsenakis, M., 1993: Regulation of glycoprotein D synthesis of herpes simplex virus 1 by alpha 4 protein, the major regulatory protein of the virus, in stably transformed cell lines: effect of the relative gene copy numbers. Earlier studies concerning gamma 1 gene regulation by the alpha 4 protein, the major regulatory protein of herpes simplex virus 1 (HSV-1), in stably transformed cell lines, reported conflicting results, i.e., alpha 4 protein positively regulated t...

Kristie, T.M.; Roizman, B., 1986: Alpha 4, the major regulatory protein of herpes simplex virus type 1, is stably and specifically associated with promoter-regulatory domains of alpha genes and of selected other viral genes. Herpes simplex virus type 1 genes form at least five groups (alpha, beta 1, beta 2, gamma 1, and gamma 2) whose expression is coordinately regulated and sequentially ordered in a cascade fashion. Previous studies have shown that functional alpha 4...

Kristie, T.M.; Roizman, B., 1986: DNA-binding site of major regulatory protein alpha 4 specifically associated with promoter-regulatory domains of alpha genes of herpes simplex virus type 1. Herpes simplex virus type 1 genes form at least five groups (alpha, beta 1, beta 2, gamma 1, and gamma 2) whose expression is coordinately regulated and sequentially ordered in a cascade fashion. Previous studies have shown that functional alpha 4...

Kristie T.M.; Roizman B., 1987: The binding of the major regulatory protein alpha 4 to the promoter regulatory domains of herpes simplex virus 1 genes. Granner, D , M G Rosenfeld And S Chang (Ed ) Ucla (University Of California-Los Angeles) Symposia on Molecular And Cellular Biology New Series, Vol 52 Transcriptional Control Mechanisms; Cetus-Ucla Conference, Keystone, Colorado, Usa, April 6-13, 1986 Xx+496p Alan R Liss, Inc : New York, New York, Usa Illus 159-170

Mackem, S.; Roizman, B., 1982: Regulation of alpha genes of herpes simplex virus: the alpha 27 gene promoter-thymidine kinase chimera is positively regulated in converted L cells. In cells infected with herpes simplex virus 1, the expression of viral genes is coordinately regulated and sequentially ordered; the alpha genes are expressed first and are followed by beta and gamma genes in a cascade fashion. Earlier, this labor...

Shapira, M.; Homa, F.L.; Glorioso, J.C.; Levine, M., 1987: Regulation of the herpes simplex virus type 1 late (gamma 2) glycoprotein C gene: sequences between base pairs -34 to +29 control transient expression and responsiveness to transactivation by the products of the immediate early (alpha) 4 and 0 genes. The glycoprotein C (gC) gene of herpes simplex virus type 1 is a true late gene, in that its expression occurs late in infection with a strict requirement for viral DNA replication. Recently, we reported on gC expression during infection with muta...

Kato, K.; Jang, H.K.an; Izumiya, Y.; Cai, J.S.un; Tsushima, Y.; Miyazawa, T.; Kai, C.; Mikami, T., 1999: Identification of the Mareks disease virus serotype 2 genes homologous to the glycoprotein B , ICP185 and major DNA-binding protein genes of herpes simplex virus type 1. We determined the nucleotide sequence of non-pathogenic Marek's disease virus serotype 2 (MDV2) strain HPRS24 glycoprotein B (gB) (UL27), ICP18.5 (UL28) and major DNA-binding protein (MDBP) (UL29) genes homologous to herpes simplex virus type...

Kato, K.; Jang, H.K.; Izumiya, Y.; Cai, J.S.; Tsushima, Y.; Miyazawa, T.; Kai, C.; Mikami, T., 1999: Identification of the Marek's disease virus serotype 2 genes homologous to the glycoprotein B (UL27), ICP18.5 (UL28) and major DNA-binding protein (UL29) genes of herpes simplex virus type 1. Nucleotide sequence of non-pathogenic Marek's disease virus serotype 2 (MDV2) strain HPRS24 glycoprotein B (gB) (UL27), ICP18.5 (UL28) and major DNA-binding protein (MDBP) (UL29) genes homologous to herpes simplex virus type 1 were determined...

Longnecker R.; Roizman B., 1986: Generation of an inverting herpes simplex virus 1 mutant lacking the l s junction alpha sequences an origin of dns synthesis and several genes including those specifying glycoprotein e and the alpha 47 gene. The herpes simplex virus genome consists of two components, L and S, that invert relative to each other to yield four isomeric arrangements, prototype (P), inversion of the S component (Is), inversion of the L component (Il), and inversion of both...

Mackem, S.; Roizman, B., 1982: Structural features of the herpes simplex virus alpha gene 4, 0, and 27 promoter-regulatory sequences which confer alpha regulation on chimeric thymidine kinase genes. Previous studies have shown that herpes simplex virus genes form three groups, alpha, beta, and gamma, whose expression is coordinately regulated and sequentially ordered in a cascade fashion. Chimeric genes constructed by fusion of the coding and...