+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Regulation of glycosamino glucan synthesis by thyroid hormone in vitro

, : Regulation of glycosamino glucan synthesis by thyroid hormone in vitro. Journal of Clinical Investigation 70(5): 1066-1073

Human skin fibroblasts synthesize and accumulate glycosaminoglycans (GAG). Fibroblasts incubated in thyroid hormone-deficient media accumulate more GAG than do cultures incubated in the same media enriched with 0.1 .mu.M triiodothyronine (T3). The current study characterizes that enhanced accumulation. Confluent cultures were maintained in thyroid hormone-deficient media without or with added T3, labeled with [3H]acetate and analyzed for total [3H]GAG and [3H]hyaluronic acid content. Addition of T3 to thyroid hormone-depleted media consistently inhibited the incorporation of [3H]acetate into GAG by 28-60% in fibroblast cultures from 4 different normal human donors. Maximal inhibitory effect was observed within 3 d after hormone addition at concentrations >n 1 nM. Of the maximal inhibitory effect, 73% was observed in the presence of physiologic concentrations of T3 (0.16 nM total T3 or 1.4 pM free T3). T3 inhibition of [3H]GAG accumulation is most likely due to a decrease in GAG synthesis rather than to changes in the acetate pool or GAG degradation; addition of 0, 100, 500 and 2500 .mu.M unlabeled acetate progressively decreased [3H]acetate incorporation into GAG, up to 80%, without altering the further inhibitory effect of T3 (35-40%); a similar effect of T3 on GAG (32% inhibition) was observed using [3H]glucosamine as substrate; T3 decreased hyaluronate synthetase activity by 32%; and there was no effect of T3 on GAG degradation in a pulse-chase experiment. The effect of T3 on [3H]GAG accumulation appears to be quite specific, since the hormone had no effect on the incorporation of [3H]leucine into trichloroacetic acid-precipitable material. Thyroid hormone inhibits GAG accumulation in a dose-, time-dependent and reversible manner. This inhibition is apparently due to specific effects on the rate of macromolecular synthesis.

(PDF 0-2 workdays service)

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 006288223

PDF Full Text: Submit

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Wolfes, R.; Mathe, J.; Seitz, A., 1991: Forensics of birds of prey by DNA fingerprinting with 33P-labeled oligonucleotide probes. Blood samples were taken and pattern tests by DNA fingerprinting carried out using 32P-labelled oligonucleotide from 113 confiscated birds of prey of 8 species: buzzard (Buteo buteo), sparrow hawk (Accipiter nisus), snowy owl (Nyctea scandiaca), k...

Schedl W., 1983: Sawfly fauna of tunisia hymenoptera symphyta. Mitteilungen der Schweizerischen Entomologischen Gesellschaft 56(3-4): 405-417

Borcherdinc, L., 1947: Excess incidence of rickets in dystrophic infants. It has been held that dystrophic infants do not develop rickets and that arrest of growth will " cure " rickets. Data from this clinic for the years 1942 to 1945 show that this is not so. The incidence of rickets, manifest but not always...

Gonzalez, D.; Patterson, B. R.; Leigh, T. F.; Wilson, L. T., 1982: Mites: a primary food source for two predators in San Joaquin Valley cotton. In cotton fields in California, predators feed on a complex of primary and secondary pests, of which the latter may become almost beneficial in keeping predator populations alive during periods of primary pest scarcity. The arthropod fauna of cott...

Roberts, William A.; Cohen, A. Ross; Maniscalco, William M., 1994: Accelerated recognition of esophageal intubation by capnography in the NICU. Pediatric Research 35(4 PART 2): 251A

Nicola Antonio Colabufo; Francesco Berardi; Mariangela Cantore; Maria Grazia Perrone; Marialessandra Contino; Carmela Inglese; Mauro Niso; Roberto Perrone; Amalia Azzariti; Grazia Maria Simone; Letizia Porcelli; Angelo Paradiso, 2008: Small P-gp modulating molecules: SAR studies on tetrahydroisoquinoline derivatives. The development of small molecules as P-gp modulating agents and SAR studies on these ligands represented the aim of the present work. A series of 6,7-dimethoxytetrahydroisoquinoline derivatives was prepared and their ability to inhibit P-gp activ...

Wolff J.A.; Acsadi G.; Jiao S.; Jani A.; Duke D.; Williams P.; Chong W., 1991: Direct gene transfer and expression into rodent striated muscle in vivo. Cohen-Haguenauer, O And M Boiron (Ed ) Colloque Inserm (Institut National De La Sante Et De La Recherche Medicale), Vol 219 Transfert De Genes Chez L'homme; (Inserm (National Institute Of Health And Medical Research) Colloquium), Vol 219 Human Gene Transfer); International Workshop, Gouvieux-Chantilly, France, April 11-13, Xxi+364p Editions Inserm: Paris, France; John Libbey Eurotext: Montrouge, France; London, England, Uk Illus Paper 263-266

Hester, F. J., 1968: Visual contrast thresholds of the goldfish (Carassius auratus). Vision Research, 8: 1315-1336

Drici, M.D.; Ahmad, S.; Singh, N.; Ducic, I.; Glazer, R.; Morad, M.; Woosley, R. L., 1997: Gender difference in I-sK protein in isolated rabbit heart is linked to differences in I-KS in isolated myocytes. Clinical Pharmacology & Therapeutics 61(2): 203

Unwin M. J., 1984: How safe is safe enough for nuclear waste?. Coal, Gold and Base Minerals of Southern Africa 32(4): 29-39