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Regulation of gonadotropin secretion in the anterior pituitary

Philosophical Transactions of the Royal Society of London B Biological Sciences 296(1080): 73-82

Regulation of gonadotropin secretion in the anterior pituitary

Studies on the regulation of gonadotorropin secretion in dissociated pituitary cell cultures are described. Initial studies employing a ferritin-labeled analog of gonadotropin hormone releasing hormone (GnRH) to localize its receptor sites on the gonadotropin cell surface showed that while these receptor sites initially have a random monodisperse distribution, binding of the ligand causes coarse aggregation and internalization of the GnRH receptor. These events are not due to the multivalency of the ligand and probably reflect redistributive events in vivo. By using an octapeptide analog of GnRH that binds to the GnRH receptor but lacks gonadotropin releasing activity in conjunction with sequence-specific antisera it is shown that antibodies that bind the octapeptide can induce the octapeptide to release gonadotropin. Receptor aggregation apparently is important in GnRH stimulation. Finally immunocytochemical studies are described in which golgi-protein-A-antibody complexes are used to identify gonadotropins on ultrathin frozen sections of porcine pituitary cells. In porcine gonadotropin cells the majority of the secretory granules contain both luteinizing hormone and FSH.

Accession: 006288245

PMID: 6121349

DOI: 10.1098/rstb.1981.0173

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