EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Regulation of granulopoiesis by autologous intact blood t cells a comparison between their influence on blood and marrow cfu c






Dokkyo Journal of Medical Sciences 14(1): 19-26

Regulation of granulopoiesis by autologous intact blood t cells a comparison between their influence on blood and marrow cfu c

To evaluate the variability of regulatory effect of normal intact T lymphocytes (T cells) on granulopoiesis, we examined the effect of peripheral blood (PB) T cells on growth of autologous PB granulopoietic progenitor cells (colony-forming unit in culture, CFU-C) and autologous bone marrow (BM) CFU-C. Either PB or BM non-T cell fractions containing CFU-C were cocultured with autologous PB-T cells in semi-solid agar culture in the presence of an exogenous source of colony stimulating factor (CSF). Neutrophil (N) and eosinophil (Eo) aggregates (clusters and/or colonies) were counted separately. The T cells consistently promoted the formation of PB-N, PB-Eo and BM-Eo aggregates in all the studies. However, the effect of the T cells on BM-N aggregtes varied: the formation of big colonies was consistently promoted, while that of small colonies was not significantly affected. These results suggest that intact PB-T cells promote the proliferation of only a fraction of immature N progenitor cells, although they promote the growth of Eo progenitor cells regardless of the maturity.


Accession: 006288262



Related references

Tebbi, K.; Rubin, S.; Cowan, D.H.; McCulloch, E.A., 1976: A comparison of granulopoiesis in culture from blood and marrow cells of nonleukemic individuals and patients with acute leukemia. The objective of this study was to compare the concentration of committed granulopoietic progenitor cells (CFU-C) in marrow and blood. For individuals without leukemia, a highly significant correlation was observed between the concentration of CFU...

Bolwell, B.J.; Goormastic, M.; Yanssens, T.; Dannley, R.; Baucco, P.; Fishleder, A., 1994: Comparison of G-CSF with GM-CSF for mobilizing peripheral blood progenitor cells and for enhancing marrow recovery after autologous bone marrow transplant. Primed peripheral blood progenitor cells (PBPC) with hematopoietic growth factors enhance marrow engraftment after autologous bone marrow transplantation (BMT). G-CSF and GM-CSF stimulate the production of PBPC; both cytokines alone also stimulate...

Bolwell, B.; Dannley, R.; Goormastic, M.; Yanssens, T.; Baucco, P.; Andresen, S.; Lichtin, A.; Fishleder, A., 1993: A comparison of G-CSF with GM-CSF for mobilization of peripheral blood progenitor cells and for enhancement of marrow recovery post autologous bone marrow transplant. Blood 82(10 SUPPL 1): 83A

Ager, S.; Jestice, K.; Scott, M.A.; Marcus, R.E., 1994: Autologous peripheral blood progenitor cells transplantation in patients with malignant lymphoma A retrospective cost comparison with autologous bone marrow transplantation. British Journal of Haematology 87(SUPPL 1): 24

Lum, L.G.; Joshi, I.D.; Smith, M.R.; Ratanatharathorn, V.; Galoforo, S.C.; Karanes, C.; Uberti, J.P.; Sensenbrenner, L.L., 1994: Constitutive and mitogen-stimulated cytokine mRNA expression by peripheral blood mononuclear cells from autologous an allogeneic bone marrow transplant recipients is intact. The immunodeficiency that occurs after human bone marrow transplantation (BMT) leaves BMT recipients susceptible to fatal infections. Although cytokines are critical for coordinating immune responses and immune reconstitution after BMT, there are...

Lum, L.G.; Joshi, I.D.; Smith, M.R.; Ratanatharathorn, V.; Galoforo, S.C.; Karanes, C.; Uberti, J.P.; Sensenbrenner, L.L., 1994: Constitutive and mitogen-stimulated cytokine mRNA expression by peripheral blood mononuclear cells from most autologous and allogeneic bone marrow transplant recipients is intact. The immunodeficiency that occurs after human bone marrow transplantation (BMT) leaves BMT recipients susceptible to fatal infections. Although cytokines are critical for coordinating immune responses and immune reconstitution after BMT, there are...

Skodlar, J.; Nemet, D.; Labar, B.; Petrovecki, M.; Sucic, M.; Golubic Cepulic, B.; Bogdanic, V.; Mrsic, M.; Marusic, M., 1994: Influence of peripheral blood cells on haematopoietic recovery after autologous bone marrow transplantation. British Journal of Haematology 87(SUPPL 1): 45

Miyanomae T.; Tsurusawa M.; Fujita J.; Mori K.J., 1982: Role of the stromal cells in the regulation of granulopoiesis in long term bone marrow culture effects of conditioned medium on granulopoiesis in vitro. Mouse abdominal wall-conditioned medium contains a factor which stimulates granulocyte-monocyte colony formation. To elucidate the role of colony-stimulating factor and the stromal microenvironment in long-term bone marrow cultures, effects of the...

Miyanomae, T.; Tsurusawa, M.; Fujita, J.; Mori, K.J., 1982: Role of the stromal cells in the regulation of granulopoiesis in long-term bone marrow culture: effects of conditioning medium on granulopoiesis "in vitro". Mouse abdominal wall-conditioned medium contains a factor which stimulated granulocyte-monocyte colony formation. To elucidate the role of colony-stimulating factor and the stromal microenvironment in long-term bone marrow cultures, effects of the...

Vandenberghe, E.; D.E.vira, C.R.iz; Loberiza, F.R.; Conde, E.; Lopez Guillermo, A.; Gisselbrecht, C.; Guilhot, F.; Vose, J.M.; Van Biesen, K.; Rizzo, J.D.uglas; Weisenburger, D.D.; Isaacson, P.; Horowitz, M.M.; Goldstone, A.H.; Lazarus, H.M.; Schm, 2003: Outcome of autologous transplantation for mantle cell lymphoma: A study by the European Blood and bone Marrow Transplant and Autologous Blood and Marrow Transplant Registries. Mantle cell lymphoma (MCL) has an aggressive clinical course with a median survival < 3 years and is incurable with conventional chemotherapy. A large multicentre study with adequate follow-up may clarify the role of significant factors affecti...