+ Translate

Regulation of growth and somatic embryogenesis by proline and serine in suspension cultures of dactylis glomerata

, : Regulation of growth and somatic embryogenesis by proline and serine in suspension cultures of dactylis glomerata. Journal of Plant Physiology 130(1): 49-56

The regulatory effects of L-proline and L-serine on growth and somatic embryogenesis of Dactylis glomerata were studied using suspension cultures. Treatments consisted of a factorial arrangement of 4 levels of proline (0-to-50 mM) and 3 levels of serine (0-50-25 mM) in a modified Schenk and Hildebrandt (SH) medium supplemented with 30 .mu.M dicamba (3,6-dichloro-o-anisic acid) (SH-30). Proline amended SH-30 medium resulted in a slight increase in dry weight; however, SH-30 supplemented with serine only dramatically inhibited growth. Growth in media containing mixtures of proline and serine was statistically similar to or less than growth in proline alone or SH-30. Significantly fewer somatic embyros were observed in treatments containing proline or serine alone. Most mixtures of proline and serine stimulated embryogenesis with the optimum response observed with 12.5 mM of each amino acid. Embryos were formed in cultures composed of individual cells and small cell masses. Very little precocious germination was evident. Approximately 70% of the embryos formed in proline-serine treatments germinated and produced seedlings on solidified SH medium without dicamba. The final pH of the media was significantly influenced by the interaction of the amino acids. Treatments with serine alone lowered pH; whereas, proline elevated pH after 6 weeks. Initiation of suspension cultures from somatic embryos was inhibited by media containing serine; whereas, media amended with either proline, casein hydrolysate or SH-30 alone supported good growth of suspension cultures.

(PDF same-day service: $19.90)

Accession: 006288283

DOI: 10.1016/S0176-1617(87)80300-0

Submit PDF Full Text: Here

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Related references

Gray D.J.; Conger B.V.; Hanning G.E., 1984: Somatic embryogenesis in suspension and suspension derived callus cultures of dactylis glomerata. Suspension cultures were initiated from somatic embryos and embryogenic callus of D. glomerata L. in SH-30 liquid medium [Schenk and Hildebrandt (1972) containing 30 .mu.M 3,6-dichloro-o-anisic acid (dicamba)] with or without 1.5 g l-1 casein hydr...

Tchorbadjieva, M.; Odjakova, M.K., 2001: An acidic esterase as a biochemical marker for somatic embryogenesis in orchardgrass (Dactylis glomerata L.) suspension cultures. The isoenzyme pattern of esterases (EC secreted into the medium of orchardgrass (Dactylis glomerata L.) embryogenic suspension cultures during defined stages of somatic embryogenesis was compared with that of non-embryogenic suspension cu...

Zagorchev, L.; Seal, C.E.; Kranner, I.; Odjakova, M., 2012: Redox state of low-molecular-weight thiols and disulphides during somatic embryogenesis of salt-treated suspension cultures of Dactylis glomerata L. The tripeptide antioxidant γ-L-glutamyl-L-cysteinyl-glycine, or glutathione (GSH), serves a central role in ROS scavenging and oxidative signalling. Here, GSH, glutathione disulphide (GSSG), and other low-molecular-weight (LMW) thiols and their c...

Hanning, G.E., 1985: Callus formation and somatic embryogenesis from leaf cultures of orchardgrass (Dactylis glomerata L.). The frequency of production of callus and regenerated plants declined as leaf sections were taken at increasing distances from the shoot apical meristem. Somatic embryos, which arose from mesophyll tissue, showed a higher embryogenic potential tha...

Conger, B.; Trigiano, R.; Gray, D.; Mcdaniel, J., 1995: Somatic embryogenesis in orchardgrass (Dactylis glomerata L.). Somatic embryogenesis and synthetic seed 0

Gavin A.L.; Conger B.V.; Trigiano R.N., 1989: Sexual transmission of somatic embryogenesis in dactylis glomerata. A Dactylis glomerata L. genotype that produces somatic embryos in vitro was tested for the ability to sexually transmit the embryogenic trait. Reciprocal crosses were performed between the embryogenic and two non-embryonic genotypes. Leaf segments...

Tchorbadjieva, M.I.; Pantchev, I.Y., 2004: DNA methylation and somatic embryogenesis of orchardgrass (Dactylis glomerata L.). Coupled restriction digestion with HpaII/MspI and EcoRII/BstNI was performed to detect the methylation level of CCGG and CC(A/T)GG sites, respectively, in DNA of leaf explants, embryogenic and non-embryogenic calli as well as of various morphologi...

Bell, L.M.; Trigiano, R.N.; Conger, B.V., 1993: Relationship of abscisic acid to somatic embryogenesis in Dactylis glomerata. The influence of abscisic acid (ABA) on somatic embryogenesis in orchardgrass (Dacylis glomerata L.) was studied in one embryogenic (Embryogen-P) and one nonembryogenic (I-39) genotype. Leaf segments from the basal portions of the two innermost le...

Li, Z.; Burritt, D.J.; Guy, P.L., 2001: Elimination of cocksfoot mottle virus from Dactylis glomerata L by somatic embryogenesis. Plant Biology (Rockville) : 177

Alexandrova, K.S.; Conger, B.V., 2002: Isolation of two somatic embryogenesis-related genes from orchard grass (Dactylis glomerata). Orchard grass (D. glomerata) leaf segments have a high capacity for direct embryogenesis from mesophyll cells and indirect embryogenesis through callus. Total RNA extracted from leaf cultures of embryogenic and nonembryogenic genotypes (Embryogen-...