EurekaMag.com logo
+ Translate

Regulation of growth hormone messenger rna synthesis by dexamethasone and triiodothyronine transcriptional rate and messenger rna stability changes in pituitary tumor cells


, : Regulation of growth hormone messenger rna synthesis by dexamethasone and triiodothyronine transcriptional rate and messenger rna stability changes in pituitary tumor cells. Journal of Molecular Biology 181(1): 41-62

The process was characterized by which the growth hormone (GH) gene is stimulated in rat pituitary tumor cells (GC or GH3) by the steroid hormone dexamethasone (Dex) and the thyroid hormone, L-triiodothyronine (T3). A primary transcriptional response is detected within 60 min of addition of T3 or Dex + T3 to GH-producing cells (GC or GH3). A 5-fold transcriptional stimulation of GH nuclear RNA occurs in cells cultured with serum substitute medium and induced with Dex + T3, while T3 alone induces a modest 2-3 fold stimulation. The absence of fetal calf serum from the cell culture medium does not decrease the level of transcriptional activity of the GH gene during hormone stimulation. After (24 h) addition of Dex + T3 the cytoplasmic GH mRNA shows a 50-fold increase, as measured by S1 nuclease analysis. This large accumulation of cytoplasmic GH mRNA in contrast to the relatively small changes in GH gene activity is inconsistent with solely a transcriptional mechanism of hormone induction. A change in specific GH mRNA stability may also take plase in response to Dex + T3. Transcriptional stimulation of the GH gene by Dex is insignificant except in the presence of T3.

(PDF 0-2 workdays service)

Accession: 006288294

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Diamond, D.J.; Goodman, H.M., 1985: Regulation of growth hormone messenger RNA synthesis by dexamethasone and triiodothyronine. Transcriptional rate and mRNA stability changes in pituitary tumor cells. We have characterized the process by which the growth hormone (GH) gene is stimulated in rat pituitary tumor cells (GC or GH3) by the steroid hormone dexamethasone (Dex) and the thyroid hormone, L-triiodothyronine (T3). A primary transcriptional r...

Kumara-Siri, M.H.; Surks, M.I., 1985: Regulation of growth hormone messenger rna synthesis by 3 5 3' triiodo l thyronine in cultured growth hormone producing rat pituitary tumor cells gc cells dissociation between nuclear iodothyronine receptor concentration and growth hormone messenger rna synthesis during the dna synthesis phase of the cell cycle. 3,5,3'-Triiodo-L-thyronine (T3) regulates the growth rate and GH production of cultured GC cells, a rat piutuitary tumour cell line. We have previously demonstrated a parallel increase in cellular content of DNA and nuclear T3 glucocorticoid...

Yang, J.; Tashjian, A.H., 1993: Transcriptional regulation by dexamethasone of endogenous thyrotropin-releasing hormone receptor messenger ribonucleic acid in rat pituitary GH4C1 cells. Previous molecular studies on the mechanism of regulation of TRH receptor (TRHR) mRNA used transfected cells and concluded that the major mode of regulation is via mRNA degradation. Glucocorticoid hormones induce an increase in the number of TRHRs...

Simard J.; Labrie F.; Gossard F., 1986: Regulation of growth hormone messenger rna and pro opiomelanocortin messenger rna levels by cyclic amp in rat anterior pituitary cells in culture. To understand the hormonal regulation of steady-state growth hormone (GH) mRNA levels, we investigated the interaction between somatostatin and agents known to increase intracellular cAMP activity on GH mRNA, GH synthesis, cell content of GH, and...

Hupart K.H.; Defesi C.R.; Katz C.P.; Shapiro L.E.; Surks M.I., 1990: Differential response to l triiodothyronine of anterior pituitary growth hormone messenger rna and beta thyrotropin messenger rna in a hypothyroid walker 256 carcinoma bearing rat model of nonthyroidal disease. To continue our studies on the infuence of T3 on TSH regulation in the Walker 256 carcinoma-bearing rat model of nonthyroidal disease, we measured the effect of T3 on pituitary content of .beta.TSH mRNA and rat (r) TSH in hypothyroid control (C) a...

Wong N.C.W.; Jump D.B.; Mariash C.N.; Oppenheimer J.H., 1985: Coordinate regulation of a rat hepatic messenger rna by triiodothyronine growth hormone and dexamethasone evidence for a common determinant of the prolonged lag time in response. Clinical Research 33(2 PART 1): 602A

Lamberts, S.W.; Oosterom, R.; Verleun, T.; Krenning, E.P.; Assies, H., 1984: Regulation of hormone release by cultured cells from a thyrotropin-growth hormone-secreting pituitary tumor. Direct inhibiting effects of 3,5,3'-triiodothyronine and dexamethasone on thyrotropin secretion. The regulation of TSH and GH secretion was investigated in cultured tumor cells prepared from a mixed TSH/GH secreting pituitary tumor. The tumor tissue had been removed transsphenoidally from a patient with hyperthyroidism and inappropriately hig...

Vikman K.; Carlsson B.; Billig H.; Eden S., 1991: Expression and regulation of growth hormone gh receptor messenger ribonucleic acid messenger rna in rat adipose tissue adipocytes and adipocyte precursor cells gh regulation of gh receptor messenger rna. The effects of hypophysectomy and hormonal replacement therapy on GH receptor (GH-R) gene expression was studied in rat adipose tissue with a cRNA probe corresponding to the amino-terminal of the hepatic GH-R. Male Sprague-Dawley rats, 50-65 days...

Melmed S.; Yamashita S., 1986: Insulin like growth factor i action on hypothyroid rat pituitary cells suppression of triiodothyronine induced growth hormone secretion and messenger rna levels. T3 stimulates in vitro GH secretion, whereas somatomedins have been shown to suppress GH. We, therefore, tested the effects of a recombinant human insulin-like growth factor analog (IGF-I; Thr 59) on T3-induced GH secretion. Pituitary monolayer cu...

Brocas, H.; Seo, H.; Refetoff, S.; Vassart, G.; Dumont, J.E., 1977: Translation of prolactin and growth hormone messenger RNA from rat pituitary tumour cells. Stimulation of GH mRNA activity by triiodothyronine [proceedings. Archives Internationales de Physiologie et de Biochimie 85(2): 387-387