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Regulation of growth of an interleukin 2 dependent murine t cell clone ht 2 in a defined serum free medium


, : Regulation of growth of an interleukin 2 dependent murine t cell clone ht 2 in a defined serum free medium. Cellular Immunology 93(2): 459-466

An IL-2[interleukin 2]-dependent T-cell clone (HT-2) can be grown in a serum-free medium (HB101) with defined additives at rates comparable to those which can be obtained in serum-containing medium. Cells cultured in the serum-free medium in the absence of IL-2 arrest growth in the G1 portion of the cell cycle, and these arrested cells can be stimulated to reenter the cell cycle upon the addition of IL-2 to the culture medium. Growth of these cells in the absence of serum requires the presence of IL-2 as well as other hormones and growth factors and 2-mercaptoethanol. HT-2 cells were grown continuously in the serum-free medium for periods of up to 1 mo.

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