EurekaMag.com logo
+ Translate

Regulation of growth of an interleukin 2 dependent murine t cell clone ht 2 in a defined serum free medium


, : Regulation of growth of an interleukin 2 dependent murine t cell clone ht 2 in a defined serum free medium. Cellular Immunology 93(2): 459-466

An IL-2[interleukin 2]-dependent T-cell clone (HT-2) can be grown in a serum-free medium (HB101) with defined additives at rates comparable to those which can be obtained in serum-containing medium. Cells cultured in the serum-free medium in the absence of IL-2 arrest growth in the G1 portion of the cell cycle, and these arrested cells can be stimulated to reenter the cell cycle upon the addition of IL-2 to the culture medium. Growth of these cells in the absence of serum requires the presence of IL-2 as well as other hormones and growth factors and 2-mercaptoethanol. HT-2 cells were grown continuously in the serum-free medium for periods of up to 1 mo.

(PDF 0-2 workdays service)

Accession: 006288304

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Shipley, G.D.; Abraham, R.T.; Swanson, B.M.; McHugh, Y.E.; Handwerger, B.S., 1985: Regulation of growth of an interleukin 2 (IL-2)-dependent murine T-cell clone (HT-2) in a defined serum-free medium. In this study, we demonstrate that an IL-2-dependent T-cell clone (HT-2) can be grown in a serum-free medium (HB101) with defined additives at rates comparable to those which can be obtained in serum-containing medium. Further, we show that cells...

Kajigaya, Y.; Ikuta, K.; Sasaki, H.; Matsuyama, S., 1990: Growth and differentiation of a murine interleukin-3-producing myelomonocytic leukemia cell line in a protein-free chemically defined medium. We established the continuous growth of WEHI-3B D+ cells in protein-free chemically defined F-12 medium by stepwise decreases in the concentration of fetal calf serum. This cell line, designated as WEHI-3B-Y1, has now been propagated in protein-fr...

Amano, T., 1978: Establishment of mouse neuroblastoma clone N18, rat glioma clone C6, and rat striated muscle clone L6 in serum-free, chemically defined medium. Mouse neuroblastoma clone N18, rat glioma clone C6, and rat striated muscle clone L6 were grown in a serum-free Dulbecco's modified Eagle's medium. Their doubling times were 48 hours, 40 hours, and 4 days, respectively. Morphologic featu...

Eby, J.E.; Sato, H.; Sirbasku, D.A., 1993: Apotransferrin stimulation of thyroid hormone dependent rat pituitary tumor cell growth in serum-free chemically defined medium: role of FE(III) chelation. Triiodothyronine (T3) dependent growth of GH1 rat pituitary tumor cells in serum-free defined culture requires apotransferrin (apo Tf) (Sirbasku et al.: Mol. Cell. Endocrinol., 77:C47–C55, 1991). Diferric transferrin (2Fe · Tf) also i...

Eldridge, M.G.; Dailey, H.A., 1992: Serum-free, defined medium for the growth and differentiation of murine erythroleukemia cells. Murine erythroleukemia (MEL) cells are frequently employed to study both cell growth and erythroid differentiation. Although these cells are easily cultured and induced to differentiate, they are routinely maintained in a medium that contains 10%-...

Eby, J., E.; Sato, H.; Sirbasku, D., A., 1993: Apotransferrin stimulation of thyroid hormone dependent rat pituitary tumor cell growth in serum-free chemically defined medium: Role of iron(III) chelation. Triiodothyronine (T-3) dependent growth of GH-1 rat pituitary tumor cells in serum-free defined culture requires apotransferrin (apoTf) (Sirbasku et al.: Mol. Cell. Endocrinol., 77:C47-C55, 1991). Diferric transferrin (2Fe cntdot Tf) also is neces...

Ropke C.; Van Deurs B.; Pedersen O.W., 1989: Culture of murine and human thymic epithelial cells in a growth factor defined serum free medium. Scandinavian Journal of Immunology 30(5): 647

Nygaard, S.J.cob Tjoflaat; Laerum, O.D.drik; Tysnes, O.B.orn, 1994: Effects of bradykinin and calcium ionophore on glioma cell migration, spheroid growth, cell growth and invasion in a serum-free defined medium. Proceedings of the American Association for Cancer Research Annual Meeting 35(0): 30

Kaighn M.E.; Kirk D.; Szalay M.; Vener G.; Lechner J.F., 1981: Population dependent clonal growth of human prostatic carcinoma cells in hormone and serum free defined medium. Proceedings of the American Association for Cancer Research & American Society of Clinical Oncology 22: 9

Kumar, S., 1983: Growth of neural cell cultures in a chemically defined, serum-free culture medium. The composition of a serum-free, completely chemically defined culture medium which supports active growth of dissociated neural-cells in culture is described. This serum-free medium can also be used to grow many types of human cell lines without...