EurekaMag.com logo
+ Translate

Regulation of heme synthesis in hepg2 human hepatoma cells by dmso


, : Regulation of heme synthesis in hepg2 human hepatoma cells by dmso. Biochemical & Biophysical Research Communications 153(2): 869-874

Dimethyl sulfoxide (DMSO) treatment of human HepG2 hepatoma cells increases the activity and the concentration of .delta.-aminolevulinic acid dehydratase (ALAD) to a comparable degree. Results of experiments with transcriptional inhibitors suggest that the increase in ALAD reflects de novo synthesis of the enzyme resulting from transcriptional activation. Commitment to increased ALAD activity in HepG2 cells is seen after 18 hr and complete by 48 hr. In contrast to the effects on ALAD, DMSO decreases the activities of porphobilinogen deaminase and uroporphyrinogen decarboxylase.

(PDF 0-2 workdays service)

Accession: 006288345

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Galbraith, R.A.; Sassa, S.; Fujita, H., 1988: Regulation of heme synthesis in HepG2 human hepatoma cells by dimethyl sulfoxide. Dimethyl sulfoxide (DMSO) treatment of human HepG2 hepatoma cells increases the activity and the concentration of delta-aminolevulinic acid dehydratase (ALAD) to a comparable degree. Results of experiments with transcriptional inhibitors suggest t...

Kappas A., 1986: Heme synthesis in human hepatoma cells in culture transcriptional activation by dmso. Clinical Research: 649a

Galbraith R.A.; Sassa S.; Kappas A., 1986: Induction of heme synthesis in hep g 2 human hepatoma cells by dmso a transcriptionally activated event. Exposure of cultured human hepatoma cells (Hep G2) to medium containing 2% (v/v) dimethyl sulphoxide resulted in an approximate doubling in the activity of .delta.-aminolaevulinate dehydratase, an increase in the haem content and a decreased growt...

Sassa, S.; Iwasa, F.; Galbraith, R., 1989: The effect of dimethyl sulfoxide on heme synthesis and the acute phase reaction in human HepG2 hepatoma cells. Effects of DMSO on heme synthesis and enzymes of the heme biosynthetic pathway were examined in human HepG2 hepatoma cells. HepG2 cells contain measurable levels of ALA synthase and ALA dehydratase, and their levels are increased after treatment o...

Iwasa, F.; Galbraith, R.A.; Sassa, S., 1990: Phenotypic variation in human HepG2 hepatoma cells: alterations in cell growth, plasma protein synthesis and heme pathway enzymes. 1. Growth rates, morphology, plasma protein synthesis and the level of heme pathway enzymes were examined in six sublines of HepG2 cells obtained from various laboratories. 2. Five sublines represented by G2a display the known characteristics of H...

Cooper, A.D.; Craig, W.Y.; Taniguchi, T.; Everson, G.T., 1994: Characteristics and regulation of bile salt synthesis and secretion by human hepatoma HepG2 cells. Bile salt uptake, synthesis and secretion by the human hepatoma-derived cell line HepG2 were studied. The cells transported and secreted bile salts largely by means of passive mechanisms. The cells synthesized and secreted the normal human primary...

Cooper, A.D.; Craig, W.Y.; Taniguchi, T.; Everson, G.T., 1994: Characteristics and regulation of bile salt synthesis and secretion by human hepatoma hepG2 cells. Bile salt uptake, synthesis and secretion by the human hepatoma-derived cell line HepG2 were studied. The cells transported and secreted bile salts largely by means of passive mechanisms. The cells synthesized and secreted the normal human primary...

Iwasa F.; Sassa S.; Kappas A., 1989: The effects of acute phase inducers and dmso on delta aminolevulinate synthase activity in human hepg2 hepatoma cells. The effects of acute-phase inducers and dimethyl sulphoxide (Me2SO) on .delta.-aminolaevulinate (ALA) synthase in HepG2 cells were examined. Treatment of cells with Me2SO resulted in a significant increase in ALA synthase activity. Interleukin-6 i...

Koj, A.; Korzus, E.; Baumann, H.; Nakamura, T.; Travis, J., 1993: Regulation of synthesis of some proteinase inhibitors in human hepatoma cells HepG2 by cytokines, hepatocyte growth factor and retinoic acid. Subconfluent monolayers of human hepatoma HepG2 cells were cultured for 2 days in serum-free DMEM containing 1 microM dexamethasone and human recombinant hepatocyte growth factor (HGF), retinoic acid (RA), IL-1, IL-6, LIF and mixtures of these fac...

Kordula, T.; Güttgemann, I.; Rose-John, S.; Roeb, E.; Osthues, A.; Tschesche, H.; Koj, A.; Heinrich, P.C.; Graeve, L., 1992: Synthesis of tissue inhibitor of metalloproteinase-1 (TIMP-1) in human hepatoma cells (HepG2). Up-regulation by interleukin-6 and transforming growth factor beta 1. Metalloproteinases and their specific inhibitors, believed to play a role in extracellular matrix metabolism, are regulated by inflammatory cytokines. Here we have addressed the question of whether liver, the major site of synthesis of plasma prot...