+ Translate

Regulation of hemopoietic cell differentiation and proliferation

, : Regulation of hemopoietic cell differentiation and proliferation. Journal of Supramolecular Structure 8(4): 489-500

Differentiation and proliferation of almost all hemopoietic cell lines can now be studied in vitro. Cloning techniques and suspension cultures allow the study of proliferation of the multipotential hemopoietic progenitor cell and the committed progenitors for granulocytes, macrophages, eosinophils, megakaryocytes and erythrocytes. The proliferation of each of the committed progenitor cells is controlled by specific glycoproteins and 2 of these have recently been purified: granulocyte-macrophage colony-stimulating factor (GM-CSF) and erythropoietin. The rate of proliferation of the GM-progenitor cells and their pattern of differentiation depends on the concentration of the hormone. At low concentrations of GM-CSF (10-11 M) fewer progenitor cells are stimulated and macrophage colonies rather than granulocyte colonies develop. The change in the direction of granulocyte-macrophage differentiation appears to be related to the concentration of GM-CSF and the different sensitivity of a subpopulation of monocyte colony-forming cells which are responsive to GM-CSF even at low concentrations of the regulator. Analysis of the rate of RNA synthesis by bone marrow cells has shown that GM-CSF stimulates the mature nondividing end cells of differentiation (i.e., polymorphs) as well as the progenitor cells. Although GM-CSF and erythropoietin have been radiolabeled, binding studies have been hampered by the loss of biologic activity during the labeling procedure and the heterogeneity of the target cells to which the regulators bind. Surface proteins and receptors for erythrocytes have been well characterized but the relationships between these proteins and the cell surface proteins of nucleated blood cells is not well understood. It appears that some proteins are lost from the cell surface during the development of granulocytes, which are retained on the surface of the B [bone-marrow derived] lymphocyte. Other proteins such as chemotactic receptors and complement receptors only appear on the mature cells. External radiolabeling of the granulocyte surface using iodogen yielded a simple profile of 125I-labeled proteins when analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis.

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 006288368

PMID: 309973

DOI: 10.1002/jss.400080411

Download PDF Full Text: Regulation of hemopoietic cell differentiation and proliferation

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Maisin, D.; Lepoutre, T.; Gruson, D.; Wallemacq, P., 2013: Quantification of serum free light chain kappa and lambda by the SPAPLUS analyser. Clinical assessment of the SPA<inf loc="post">PLUS</inf> system for the determination of the serum free light chains Kappa (? FLC) and Lambda (? FLC) compared to the BNII . 126 serum specimen from our routine activity were an...

Zambrano, A.I.; Muñoz, B.E.; Mkocha, H.; West, S.K., 2016: Exposure to an Indoor Cooking Fire and Risk of Trachoma in Children of Kongwa, Tanzania. Elimination of blinding trachoma by 2020 can only be achieved if affected areas have effective control programs in place before the target date. Identifying risk factors for active disease that are amenable to intervention is important to successf...

Camilleri, A., 2001: Venous obstruction caused by a self-adhesive drape: a cautionary tale. The use of disposable drapes during operations is becoming more widespread, and their advantages are well documented. I describe how use of such a drape led to venous constriction which caused troublesome oozing from the operative sites during sag...

Mårdh, P.A., 2000: Chlamydia screening--yes, but of whom, when, by whom, and with what?. The importance of screening programs in reducing the prevalence of genital chlamydial infections is stressed by the fact that the majority of infected persons are more or less asymptomatic. The use of oral contraceptives may mask infections affect...

Kakkos, S.K.; Kirkilesis, G.I.; Tsolakis, I.A., 2015: Re: 'Re. Efficacy and Safety of the New Oral Anticoagulants Dabigatran, Rivaroxaban, Apixaban, and Edoxaban in the Treatment and Secondary Prevention of VTE: A Systematic Review and Meta-analysis of Phase III Trials'. European Journal of Vascular and Endovascular Surgery 50(1): 127-127

Will, G. M., 1968: Some aspects of organic matter formation and decomposition in pumice soils growing Pinus radiata forest. A review of the decomposition of litter from P. radiata, of "bleaching" in the A1 horizon produced by fungal activity and of the organic matter content of fossil pumiceous soils.

Martin Rudolf; Jamin Frank G.S., 1963: Paleogeomorphology of the buried Devonian landscape in northeastern Alberta. Information Series - Alberta Research Council Pages 31-42

Covas, D.T.; Siufi, J.L.C.; Silva, A.R.L.; Orellana, M.D., 2003: Isolation and culture of umbilical vein mesenchymal stem cells. Bone marrow contains a population of stem cells that can support hematopoiesis and can differentiate into different cell lines including adipocytes, osteocytes, chondrocytes, myocytes, astrocytes, and tenocytes. These cells have been denoted mesen...

Lukes, S., 1984: Immune response in rabbits experimentally infected with Ascaris suum. Immune response of rabbits experimentally infected with A. suum was studied by indirect hemagglutination method. The animals were infected with the doses of 5000, 10,000 and 20,000 infective eggs per animal. Positive reactions were observed from d...

Gao, S.; Zhang, L.; Xu, Y.; Lu, P.; Chen, L.; Bao, X., 2016: Tapered fiber based Brillouin random fiber laser and its application for linewidth measurement. NlmCategory="UNASSIGNED">A one-end pumping Brillouin random fiber laser (BRFL) based on a 5-km tapered fiber (TF) is demonstrated. The enhanced Rayleigh scattering and the increased power density from tapering in the TF provide good d...