EurekaMag.com logo
+ Site Statistics
References:
48,253,228
Abstracts:
22,798,842
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Regulation of hemopoietic cell differentiation and proliferation


, : Regulation of hemopoietic cell differentiation and proliferation. Journal of Supramolecular Structure 8(4): 489-500

Differentiation and proliferation of almost all hemopoietic cell lines can now be studied in vitro. Cloning techniques and suspension cultures allow the study of proliferation of the multipotential hemopoietic progenitor cell and the committed progenitors for granulocytes, macrophages, eosinophils, megakaryocytes and erythrocytes. The proliferation of each of the committed progenitor cells is controlled by specific glycoproteins and 2 of these have recently been purified: granulocyte-macrophage colony-stimulating factor (GM-CSF) and erythropoietin. The rate of proliferation of the GM-progenitor cells and their pattern of differentiation depends on the concentration of the hormone. At low concentrations of GM-CSF (10-11 M) fewer progenitor cells are stimulated and macrophage colonies rather than granulocyte colonies develop. The change in the direction of granulocyte-macrophage differentiation appears to be related to the concentration of GM-CSF and the different sensitivity of a subpopulation of monocyte colony-forming cells which are responsive to GM-CSF even at low concentrations of the regulator. Analysis of the rate of RNA synthesis by bone marrow cells has shown that GM-CSF stimulates the mature nondividing end cells of differentiation (i.e., polymorphs) as well as the progenitor cells. Although GM-CSF and erythropoietin have been radiolabeled, binding studies have been hampered by the loss of biologic activity during the labeling procedure and the heterogeneity of the target cells to which the regulators bind. Surface proteins and receptors for erythrocytes have been well characterized but the relationships between these proteins and the cell surface proteins of nucleated blood cells is not well understood. It appears that some proteins are lost from the cell surface during the development of granulocytes, which are retained on the surface of the B [bone-marrow derived] lymphocyte. Other proteins such as chemotactic receptors and complement receptors only appear on the mature cells. External radiolabeling of the granulocyte surface using iodogen yielded a simple profile of 125I-labeled proteins when analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis.

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 006288368

PMID: 309973

DOI: 10.1002/jss.400080411

PDF Full Text: Regulation of hemopoietic cell differentiation and proliferation


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Puls, K.L.; Ni, J.; Liu, D.; Morahan, G.; Wright, M.D., 1999: The molecular characterisation of a novel tetraspanin protein, TM4-B(1). TM4-B is a novel member of the Tetraspanin superfamily and displays characteristics typical of the superfamily. It bears significant homology to other superfamily members and is most similar to Tspan-1. This molecule is broadly expressed in most h...

Kim SungOk; Lee, H.E.J.; Choe WonKyung, 2006: The effects of ginseng saponin-Re, Rc and green tea catechine; ECGC (epigallocatechin gallate) on leptin, hormone sensitive lipase and resistin mRNA expressions in 3T3-L1 adipocytes. A study was conducted to examine the effects of treatment with ginsenoside Re, Rc and epigallocatechin gallate (EGCG) on mRNA expressions of leptin, hormone sensitive lipase (HSL) and resistin in 3T3-L1 adipocytes. EGCG at concentrations 0.01x10-7...

Sturm, J.A.; Lewis, F.R.; Elings, V.B., 1979: Bedside determination of extravascular lung water. Extravascular lung water (EVLW) was measured at the bedside in 12 patients with the thermal-green dye double indicator dilution method using a microprocessor. The EVLW ranged from 3.3 to 17.2 ml/kg body weight; in patients without pulmonary proble...

Phillips, P.; Wilson, D.; Wakefield, M.; Beilby, J., 1990: Death and diabetes. Medical Journal of Australia 153(3): 173-173

Zyryanov Ye V., 1989: Palynologic study of the Upper Cenozoic of Faddeyevskiy Island, New Siberian Islands. Izvestiya Akademii Nauk SSSR Seriya Geologicheskaya

Schwolow, R.; Wilckens, E.; Roth, N., 1988: Effect of transcutaneous nerve stimulation (TENS) on dental pain: comparison of psychophysical and neurophysiological data and application in dentistry. Activitas Nervosa Superior 30(2): 129-130

Banarescu, P., 1964: Pisces-Osteichthyes. Fauna Republicii Populare Romine, 13: 1-962

Annuar, M.; Tan, I.; Ibrahim, S.R.machandran, K., 2007: Production of medium-chain-length poly (3-hydroxyalkanoates) from crude fatty acids mixture by Pseudomonas putida. Food and bioproducts processing: transactions of the Institution of Chemical Engineers Part C 85(2): 104-119

Dovizio, M.; Alberti, S.; Sacco, A.; Guillem-Llobat, P.; Schiavone, S.; Maier, T.J.; Steinhilber, D.; Patrignani, P., 2016: Novel insights into the regulation of cyclooxygenase-2 expression by platelet-cancer cell cross-talk. Platelets are activated by the interaction with cancer cells and release enhanced levels of lipid mediators [such as thromboxane (TX)A2 and prostaglandin (PG)E2, generated from arachidonic acid (AA) by the activity of cyclooxygenase (COX)-1], gran...

Bernaudin, J.F.; Atassi, K.; Lancret, P.; Pinchon, M.C.; Bignon, J., 1981: Recent advances in methods for morphological study of pleuro-pulmonary diseases (excluding bronchial cancers). La Revue du Praticien 31(1): 21-26