EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Regulation of hemopoietic stem cell proliferation in long term bone marrow cultures


Biomedicine Express (Paris) 27(9-10): 344-349
Regulation of hemopoietic stem cell proliferation in long term bone marrow cultures
The development of a suitable [mouse] bone marrow derived adherent cell population appears to be essential for the prolonged maintenance of hemopoietic stem cells in vitro. When established adherent layers are inoculated with freshly isolated bone marrow cells, proliferation of stem cells (CFU-S) regularly occurs in the adherent layer and among the non-adherent cells. CFU-S present within the adherent layer are able to regenerate themselves and the non-adherent CFU-S. One day after re-feeding the cultures (by removal of half the growth medium and addition of fresh medium) the 'non-adherent' and the 'adherent' CFU-S are in a high cycling state (greater than 40% kill with 3H-TdR [tritiated-thymidine]. This proportion decreases with time after re-feeding and 5-7 days later the majority of 'adherent' and 'non-adherent' CFU-S are in a low cycling state (< 10% 3H-TdR kill). Following a further re-feeding, CFU-S again enter a high cycling state.


Accession: 006288369

PMID: 606310



Related references

Evidence for direct cell cell interactions in the regulation of hemopoietic stem cell proliferation in long term human marrow cultures. Clinical & Investigative Medicine 10(4 SUPPL B): B84, 1987

Regulation of haemopoietic stem cell proliferation in long term bone marrow cultures. Biomedicine / 27(9-10): 344-349, 1977

The regulation of hemopoiesis in long-term bone marrow cultures. II. Stimulation and inhibition of stem cell proliferation. Blood 55(6): 931-936, 1980

The regulation of hemopoiesis in long term bone marrow cultures 2. stimulation and inhibition of stem cell proliferation. Blood 55(6): 931-936, 1980

Stromal regulation of hemopoietic stem cells in long-term human bone marrow tissue cultures under the effect of parathyroid hormone. Bulletin of Experimental Biology and Medicine 142(4): 527-530, 2007

In vitro analysis of the contributions of adherent cell layer and recharging population to hemopoietic cell proliferation in long-term bone marrow cultures. Experimental Hematology 9(4): 319-325, 1981

The role of growth factors in the regulation of primitive hemopoietic cell proliferation in the adherent layer of long term human marrow cultures. Journal of Leukocyte Biology 42(5): 606, 1987

In vitro proliferation of hemopoietic stem cells in long-term marrow cultures: principles in mouse applied to man. Experimental Hematology 7 Suppl 5: 135-148, 1979

Proliferation of precursor cells of the hemopoietic microenvironment in long-term mouse bone marrow cultures. Biulleten' Eksperimental'noi Biologii i Meditsiny 98(11): 612-614, 1984

Proliferation of hemopoietic microenvironment precursor cells in long term murine bone marrow cultures. Byulleten' Eksperimental'noi Biologii i Meditsiny 98(11): 612-614, 1984