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Regulation of hemopoietic stem cell proliferation in long term bone marrow cultures


, : Regulation of hemopoietic stem cell proliferation in long term bone marrow cultures. Biomedicine Express (Paris) 27(9-10): 344-349

The development of a suitable [mouse] bone marrow derived adherent cell population appears to be essential for the prolonged maintenance of hemopoietic stem cells in vitro. When established adherent layers are inoculated with freshly isolated bone marrow cells, proliferation of stem cells (CFU-S) regularly occurs in the adherent layer and among the non-adherent cells. CFU-S present within the adherent layer are able to regenerate themselves and the non-adherent CFU-S. One day after re-feeding the cultures (by removal of half the growth medium and addition of fresh medium) the 'non-adherent' and the 'adherent' CFU-S are in a high cycling state (greater than 40% kill with 3H-TdR [tritiated-thymidine]. This proportion decreases with time after re-feeding and 5-7 days later the majority of 'adherent' and 'non-adherent' CFU-S are in a low cycling state (< 10% 3H-TdR kill). Following a further re-feeding, CFU-S again enter a high cycling state.

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Holden, J.A.; Kelley, W.N., 1978: Human hypo xanthine guanine phospho ribosyl transferase ec 2.4.2.8 evidence for tetrameric structure. Hypoxanthine-guanine phosphoribosyltransferase (EC 2.4.2.8) was purified 23,000-fold from normal human erythrocytes. The purification includes affinity chromatography on a GMP column. The subunit MW of the enzyme obtained from this purification is...

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