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Regulation of hepatic phosphoenol pyruvate carboxy kinase ec 4.1.1.32 role of dietary proteins and amino acids in vivo and in the isolated perfused rat liver


, : Regulation of hepatic phosphoenol pyruvate carboxy kinase ec 4.1.1.32 role of dietary proteins and amino acids in vivo and in the isolated perfused rat liver. Biochimica et Biophysica Acta 632(4): 473-482

The effect of protein feeding and the addition of amino acids on the activity of hepatic phosphoenolpyruvate carboxykinase (GTP: oxaloacetate carboxylyase (transphosphorylating), EC 4.1.1.32) was investigated in vivo and in the isolated perfused rat liver. Protein feeding resulted in a considerable increase in phosphoenolpyruvate carboxykinase activity within 6 h. This rise was independent of the presence of glucocorticoids. In the isolated perfused liver system amino acids had a small effect on phosphoenolpyruvate carboxykinase activity and led to an increase by 20% when glucocorticoids were present, but resulted in a rise by 100% when glucocorticoids plus dibutyryl cAMP were added to the perfusion medium. The effect of amino acids in the presence of dibutyryl cAMP could be observed in the liver of glucocorticoid-deprived rats. Cycloheximide, a translational inhibitor, totally blocked all effects of amino acids on enzyme activity. The concentration of amino acids in the portal vein modify the regulation of phosphoenolpyruvate carboxykinase by cAMP.

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Related references

Seitz, H.J.; Tiedgen, M.; Tarnowski, W., 1980: Regulation of hepatic phosphoenolpyruvate carboxykinase (GTP). Role of dietary proteins and amino acids in vivo and in the isolated perfused rat liver. The effect of protein feeding and the addition of amino acids on the activity of hepatic phosphoenolpyruvate carboxykinase (GTP : oxalacetate carboxylyase (transphosphorylating), EC 4.1.1.32) was investigated in vivo and in the isolated perfused r...

Kramer J.W.; Freedland R.A., 1971: The dietary control of gluconeogenesis by the ratio of pyruvate kinase phosphoenol pyruvate carboxy kinase activity in the perfused rat liver. Federation Proceedings 30(2): 401

Krone, W.; Marquardt, W.; Seitz, H.J.; Tarnowski, W., 1976: Interaction between gluco corticoids and cyclic amp in the regulation of phosphoenol pyruvate carboxy kinase gtp ec 4.1.1.32 in the isolated perfused rat liver effects of cordycepin and cyclo heximide. The mechanism of the interaction between glucocorticoids and cyclic[c]AMP in the regulation of phospho[p]enolpyruvate carboxykinase (GTP: oxaloacetate carboxylyase, transphosphorylating, EC 4.1.1.32) was investigated in the isolated perfused rat l...

Ballard, F.J.; Hanson, R.W., 1969: Purification of phosphoenol pyruvate carboxy kinase from the cytosol fraction of rat liver and the immunochemical demonstration of differences between this enzyme and the mitochondrial phosphoenol pyruvate carboxy kinase. Journal of Biological Chemistry 244(20): 5625-5630

Medrano L.; Ruiz Amil M.; Losada M., 1969: Effect of glucose on pyruvate utilization by rhodotorula glutinis enz pyruvate kinase enz phosphoenol pyruvate carboxy kinase inst auto radiography alanine aspartate amino acids. Archiv fuer Mikrobiologie 66(3): 239-249

Anonymous, 1975: Effect of cordycepin and cyclo heximide on the induction of phosphoenol pyruvate carboxy kinase ec 41132 by dexamethasone or n 6 o 2 di butyryl cyclic amp in the isolated perfused rat liver

Colin W.W.; Schofield P.J.; Johnstone I.L., 1968: Carbon di oxide fixation in haemonchus contortus larvae enz phosphoenol pyruvate carboxy kinase enz malic enz phosphoenol pyruvate carboxylase enz phosphoenol pyruvate carboxy trans phosphorylase. Comparative Biochemistry & Physiology 26(2): 537-544

Zammit, V.A.; Newsholme, E.A., 1978: Properties of pyruvate kinase ec 2.7.1.40 and phosphoenol pyruvate carboxy kinase ec 4.1.1.32 in relation to the direction and regulation of phosphoenol pyruvate metabolism in muscles of the frog and marine invertebrates. The properties of pyruvate kinase (EC 2.7.1.40) and, if present, phosphoenolpyruvate carboxykinase (EC 4.1.1.32) from the muscles of the sea anemone [Metridium senile], scallop [Pecten maximus], oyster [Ostrea edulis], crab [Limulus polyphemus], l...

Arinze, I.J.; Garber, A.J.; Hanson, R.W., 1973: The regulation of gluconeogenesis in mammalian liver the role of mitochondrial phosphoenol pyruvate carboxy kinase ec 4.1.1.32. Journal of Biological Chemistry 248(7): 2266-2274

Hee-Lee, M.; Hebda, C.A.; Nowak, T., 1981: Role of cations in avian liver phosphoenol pyruvate carboxy kinase ec 4.1.1.32 catalysis activation and regulation. The activation of the avian [chicken] liver phosphoenolpyruvate carboxykinase-catalyzed reversible decarboxylation of oxalacetate by Mn2+ was studied. The Mn2+ facilitates the interaction of oxalacetate to the enzyme. At saturating ITP and oxalace...