EurekaMag.com logo
+ Translate

Regulation of hepatic poly adenylic acid endo nuclease by cortico sterone and amino acids


, : Regulation of hepatic poly adenylic acid endo nuclease by cortico sterone and amino acids. Journal of Biological Chemistry 254(22): 11228-11233

Poly(A)[adenylic-acid] polymerase and poly(A) endonuclease activities were measured in rat liver nuclear extracts prepared from fed and fasted rats. No differences in the levels of poly(A) polymerase activity of control and fasted nuclear extracts were found, but a 3- to 4-fold increase was observed in the level of poly(A) endonuclease in the fasted nuclear extracts when compared to fed controls. Activities of the cytosol poly(A) polymerase or poly(A) endonuclease or of 2 other nuclear RNase, an 3',5'-exonuclease and an alkaline RNase II-like activity were not affected by fasting, suggesting that this effect is specific for nuclear poly(A) endonuclease. Refeeding a complete amino acid mixture reduced the level of the fasting poly(a) endonuclease to control levels in 1 h. Only certain amino acids were active in causing this decrease in the fasting-induced level of poly(A) endonuclease; these include alanine, asparagine, glutamine, isoleucine, proline and serine. Hypophysectomy had no effect on the fasting-induced increase of the poly(A) endonuclease, while adrenalectomy effectively blocked the fasting-induced increase in the activity of this enzyme. When fasted adrenalectomized rats were given corticosterone, the fasting-induced increase in poly(A) endonuclease was found, but injection of corticosterone did not alter the activity of poly(A) endonuclease in control rats, suggesting that corticosterone plays a permissive role in the regulation of nuclear poly(A) endonuclease.

(PDF 0-2 workdays service)

Accession: 006288421

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Jewett P.B.; Hieter P.A.; Legenore S.M.; Dorr R.G., 1975: Possible role for poly adenylic acid as an inhibitor of endo nuclease activity in eukaryotic cells. Nature (London) 256(5515): 340-341

Alderfer J.L.; Tazawa I.; Tazawa S.; Ts'o P.O.P., 1974: Comparative studies on homo polymers of adenylic acid possessing different 2 carbon substituents of the furanose poly deoxy ribo adenylic acid poly ribo adenylic acid poly 2 o methyl adenylic acid and poly 2 o ethyl adenylic acid. Biochemistry 13(8): 1615-1622

de GASQUET.L.WY.R., 1961: Effect of adenylic nucleotides on the in vitro inversion of D-amino acids. I. Regulation of D-amino acid oxidation by adenylic nucleotides in rat kidney homogenates. Enzymologia Biologica et Clinica 1: 176-186

Sulkowski E.; Laskowski M.Sr, 1969: Recognition of structural differences between deoxy adenylic acid deoxy thymidylic acid and poly adenylic acid poly thymidylic acid by micrococcal enz nuclease abstract. Federation Proceedings 28(2): 900

Maser Gluth C.; Toygar A.; Vecsei P., 1984: Time course of plasma cortico sterone 18 hydroxy cortico sterone and aldo sterone concentrations following corticotropin releasing factor administration in the rat a phase of cortico sterone inhibition. Synthetic ovine [O] CRF [corticotropin-releasing factor] (8 .mu.g/rat), injected i.v. in nembutal anesthetized rats, increased not only plasma ACTH and corticosterone, but also aldosterone and 18-hydroxycorticosterone concentrations. The maximum e...

Salmenpera, M.; Kahri, A.I., 1976: Cortico sterone 18 hydroxydeoxy cortico sterone deoxy cortico sterone and aldo sterone secretion in tissue culture of fetal rat adrenals in the presence and the absence of acth. A method for simultaneous analysis of the main corticosteroids secreted by the rat adrenal is described. Purification is performed using previously established steps involving dichloromethane extraction, ethanol-cyclohexane partition and Sephadex-...

Matts R.A.; Siegel F.L., 1979: Regulation of rat liver poly adenylic acid polymerase and poly adenylic acid hydrolase in fasting and re feeding. Federation Proceedings 38(3 PART 1): 507

Schroeder H.C.; Zahn R.K.; Mueller W.E.G., 1982: Role of actin and tubulin in the regulation of poly adenylic acid polymerase endo rnase iv complex from calf thymus. The poly(A) polymerase from calf thymus (MW = 62,000) is associated with the poly(A)-specific endoribonuclease IV (MW = 45,000); this complex is dissociable at pH 8.2. The activities of the 2 enzymes in the complex are strongly inhibited by G-acti...

Den K.; Tomita M.; Miyake Y.; Yamaguchi N.; Fukasawa M.; Teramoto K.; Takagi S., 1980: Maternal and fetal 11 deoxy cortico sterone cortico sterone 18 hydroxy cortico sterone and aldo sterone in normal and toxemic pregnancy. Simultaneous determination of maternal and fetal plasma 11-deoxycorticosterone (DCC), corticosterone (B), 18-hydroxycorticosterone (18OH-B) and aldosterone (ALDO) in normal and toxemic pregnancies was performed by utilizing a newly developed radio...

Carlstedt Duke J.; Gustafsson J A.; Gustafsson S.A., 1975: Sexual differences in hepatic metabolism and intra cellular distribution of cortico sterone studied by pulse labeling with tritiated cortico sterone. Biochemistry 14(3): 639-648