EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Regulation of hepatic receptor dependent degradation of low density lipo proteins by mevinolin in rabbits with hyper cholesterolemia induced by a wheat starch casein diet


Biochimica et Biophysica Acta 754(2): 134-141
Regulation of hepatic receptor dependent degradation of low density lipo proteins by mevinolin in rabbits with hyper cholesterolemia induced by a wheat starch casein diet
Rabbits fed a wheat starch-casein diet develop a marked hypercholesterolemia and have a slower rate of removal of rabbit 125I-labeled low density lipoproteins (LDL) from plasma. Treating rabbits with mevinolin, a highly potent competitive inhibitor of 3-hydroxy-3-methylglutaryl-CoA reductase, at a daily dose of 20 mg/animal prevents the increase in plasma and LDL cholesterol. The mevinolin effect is mediated through an increased rate of removal of rabbit 125I-labeled LDL from plasma. To study the role of mevinolin on the regulation of the hepatic LDL receptor in rabbits, the binding of 125I-labeled LDL and 125I-labeled .beta.-VLDL (.beta.-migrating very-low-density lipoproteins) to liver membranes prepared from rabbits fed the wheat starch-casein diet with or without mevinolin was investigated. Liver membranes from wheat starch-casein-fed rabbits have no demonstrable EDTA-sensitive binding activity of 125I-labeled LDL and low (37 ng/mg protein) binding activity of 125I-labeled .beta.-VLDL. Treatment of the wheat starch-casein fed rabbits with mevinolin results in high levels of specific EDTA-sensitive binding activity of 125I-labeled LDL (28.7 ng/mg protein) and 125I-labeled .beta.-VLDL (120 ng/mg protein). To assess the functional role of the hepatic LDL receptor in response to mevinolin, the catabolism of 125I-labeled LDL by perfused rabbit livers was studied. Perfused livers from mevinolin-treated rabbits show a 3.3-fold increase in the rate of receptor-dependent catabolism of 125I-labeled LDL (4.6% .cntdot. h-1) when compared with that of livers from rabbits not treated with mevinolin (1.4% .cntdot. h-1). Thus, mevinolin prevents the increase of plasma LDL cholesterol level in rabbits fed a wheat starch-casein diet by regulating the levels of hepatic LDL-binding sites and the rate of receptor-dependent catabolism of LDL by the liver.


Accession: 006288422



Related references

Regulation of hepatic receptor-dependent degradation of LDL by mevinolin in rabbits with hypercholesterolemia induced by a wheat starch-casein diet. Biochimica et Biophysica Acta 754(2): 134-141, 1983

Hyper cholesterolemia in fasting rabbits reduction of receptor dependent low density lipo protein catabolism. American Heart Association Monograph (91): II-100, 1982

Structural and functional changes of rhesus monkey serum low density lipo proteins during cycles of diet induced hyper cholesterolemia. Arteriosclerosis 2(6): 475-486, 1982

Low density lipo protein receptor activity on skin fibroblasts from rhesus monkeys with diet induced or spontaneous hyper cholesterolemia. Journal of Biological Chemistry 255(1): 92-99, 1980

Simultaneous measurement of apo lipo protein b turnover in very low density lipo proteins and low density lipo proteins in familial hyper cholesterolemia. Atherosclerosis 28(3): 247-256, 1977

Familial hyper cholesterolemia defective hepatic low density lipo protein receptor binding with accumulation of hepatic cholesterol and cholesteryl ester. Arteriosclerosis 2(5): 421A, 1982

Effect of diet induced hyper cholesterolemia on the metabolism of apo lipo proteins a i and a ii in pigtail monkeys. Circulation Supplement 62(3): III-193, 1980

Effect of diet induced hyper cholesterolemia on the metabolism of apo lipo proteins a i and a ii in pigtail monkeys macaca nemestrina. Arteriosclerosis 1(1): 54, 1981

Regulation of lipid synthesis by low density lipo proteins in cultured skin fibroblasts in homo zygous familial hyper cholesterolemia. Biochimica et Biophysica Acta 487(3): 445-457, 1977

Defective hepatic lipo protein receptor binding of beta very low density lipo proteins from type iii hyper lipo proteinemic patients importance of apo lipo protein. Journal of Biological Chemistry 259(2): 860-869, 1984