+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Regulation of herpes simplex virus 1 genes: alpha gene sequence requirements for transient induction of indicator genes regulated by beta or late (gamma 2) promoters

, : Regulation of herpes simplex virus 1 genes: alpha gene sequence requirements for transient induction of indicator genes regulated by beta or late (gamma 2) promoters. Virology 149(2): 152-164

This laboratory reported earlier that chimeric genes consisting of the structural sequences of the thymidine kinase (TK) gene fused to the promoter-regulatory domains of late (.gamma.2) genes were regulated as bonafide .gamma.2 genes when resident in the herpes simplex virus 1 genome but could not be differentiated from .beta. genes when introduced by transfection and stably integrated into the environment of the host genome (S. Silver and B. Roizman, Mol. Cell. Biol. 5, 518-528, 1985). We report here that .beta.-TK and the chimeric .gamma.2-TK gene transfected into TK- baby hamster kidney (BHKtk-) were induced by .alpha.4 and .alpha.O but not by the other .alpha. genes. Specifically: (i) Both TK gnees were induced by cotransfection with DNA fragments carrying an intact .alpha.4 or an intact .alpha.O gene, but not by fragments carrying only the promoter-regulatory domain or the structural sequences of the .alpha.4 gene or intact .alpha.22, .alpha.27, and .alpha.47 genes. (ii) An .alpha.4 gene carrying a 2700-bp deletion in its 3' coding sequence also induced both genes, although less efficiently. (iii) RNA homologous to the .alpha.4 gene recovered from the cytoplasm of cell transfected with either the intact or truncated .alpha.4 gene mapped to the bonafide site of transcription initiation of the .alpha.4 gene. RNA homologous to the chimeric TK gene extracted from the cytoplasm of cells transfected with both .gamma.2-TK and the .alpha.4 gene was transcribed from the bonafide .gamma.2 gene capping site fused to the TK gene. These results indicate that the .alpha.4 gene and the .alpha.O gene are each capable of inducing the expression of both .beta. and .gamma.2 genes resident in the environment of the cellular genome, that the active site responsible for induction is located near the N terminus of the .alpha.4 protein, and reinforce the conclusion that .gamma.2 genes resident in the environment of the host cell cannot be used to identify the authentic determinants of .gamma.2 gene regulation by currently available tests.

(PDF 0-2 workdays service)

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 006288438

PMID: 3004024

PDF Full Text: Submit

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Reiss J., 1975: Myco toxins in foods part 4 the influence of various wrapping foils on the growth of aspergillus flavus and the formation of afla toxin b 1 and afla toxin g 1 on some types of sliced bread. Food & Cosmetics Toxicology 13(3): 325-330

Martin, R.B.; Morlino, V.J., 1965: Exchange of carbon-bound hydrogen atoms ortho to the hydroxyl group in tyrosine. The carbon-bound hydrogen atoms of tyrosine that exchange with solvent protons in strongly acid solutions at about 100[degree]C are not the methylene hydrogen atoms but a pair on the aromatic ring. Of the two pairs of protons on the aromatic ring,...

Hunt Adrian; Wolberg Donald L.; Menack Julie, 1981: Upper Cretaceous stratigraphy in Hunter Wash area, San Juan Basin, New Mexico. AAPG Bulletin 65(3): 561

Kovel, P. V., 2007: Methodical positions of estimation of economic inertia as the phenomenon and the factor of the production efficiency on agricultural enterprises. This paper outlines the content and the substantiation of methodical positions on the estimation of economic inertia, which accompanies the dynamics of expenses and results in agricultural production. Further, the paper describes the mechanism of...

Brunken, W.J.; Daw, N.W., 1987: The actions of serotonergic agonists and antagonists on the activity of brisk ganglion cells in the rabbit retina. Extracellular electrophysiological recordings were obtained from rabbit retinal ganglion cells in either a superfused eyecup or an in vivo preparation. Selective antagonists or agonists of serotonin at the 5-HT2 or 5-HT1A receptors were applied, a...

Semprucci, F.; Guerrini, A.; Balsamo, M., 2005: Preliminary data on the biodiversity of the nematode fauna from the central Adriatic Sea Dati preliminari sulla biodiversita della nematofauna dellAdriatico centrale. A faunistic and systematic research on the nematode communities of a coast trait of the Central Adriatic Sea (Marches, Italy) was carried out over two years. A total of 80 morphotypes of nematodes were collected and identified, belonging to 52 gen...

Basse, C. W.; Kolb, S.; Kahmann, R., 2002: A maize-specifically expressed gene cluster in Ustilago maydis. The pathogen U. maydis [U. zeae] requires its host plant, maize, for the development and completion of its sexual cycle. We have identified the fungal mig2-1 gene as being specifically expressed during this biotrophic stage. Intriguingly, mig2-1 i...

Shi, Y.; Melnikov, V.Y.; Schrier, R.W.; Edelstein, C.L., 2000: Downregulation of the calpain inhibitor protein calpastatin by caspases during renal ischemia-reperfusion. The interaction between the cysteine proteases calpain and caspases during renal ischemia-reperfusion (I/R) was investigated. An increase in the activity of calpain, as determined by 1) the appearance of calpain-mediated spectrin breakdown product...

J. Šajgalik, 1991: Geotechnical properties of the Danubian Lowland loess soils in the Slovak Carpathians. The Slovak Carpathians in Pleistocene presented a very articulate territory, so that loesses, as well as loess-like deposits could occur here with various lithofacial types.

Khodakov M.B.; Korets'ka Zh A.; Kahorovs'ka L.T.; Zakharchenko H.M., 1979: Some aspects of prophylaxis of drug addiction. The problem of human non-alcohol narcomania was discussed. Methods of preventing drug addiction, emphasizing the necessity of limiting access to narcotic agents by means of special restricting measures in prescribing and selling these agents, were...