EurekaMag.com logo
+ Translate

Regulation of herpes simplex virus 1 genes: alpha gene sequence requirements for transient induction of indicator genes regulated by beta or late (gamma 2) promoters


, : Regulation of herpes simplex virus 1 genes: alpha gene sequence requirements for transient induction of indicator genes regulated by beta or late (gamma 2) promoters. Virology 149(2): 152-164

This laboratory reported earlier that chimeric genes consisting of the structural sequences of the thymidine kinase (TK) gene fused to the promoter-regulatory domains of late (.gamma.2) genes were regulated as bonafide .gamma.2 genes when resident in the herpes simplex virus 1 genome but could not be differentiated from .beta. genes when introduced by transfection and stably integrated into the environment of the host genome (S. Silver and B. Roizman, Mol. Cell. Biol. 5, 518-528, 1985). We report here that .beta.-TK and the chimeric .gamma.2-TK gene transfected into TK- baby hamster kidney (BHKtk-) were induced by .alpha.4 and .alpha.O but not by the other .alpha. genes. Specifically: (i) Both TK gnees were induced by cotransfection with DNA fragments carrying an intact .alpha.4 or an intact .alpha.O gene, but not by fragments carrying only the promoter-regulatory domain or the structural sequences of the .alpha.4 gene or intact .alpha.22, .alpha.27, and .alpha.47 genes. (ii) An .alpha.4 gene carrying a 2700-bp deletion in its 3' coding sequence also induced both genes, although less efficiently. (iii) RNA homologous to the .alpha.4 gene recovered from the cytoplasm of cell transfected with either the intact or truncated .alpha.4 gene mapped to the bonafide site of transcription initiation of the .alpha.4 gene. RNA homologous to the chimeric TK gene extracted from the cytoplasm of cells transfected with both .gamma.2-TK and the .alpha.4 gene was transcribed from the bonafide .gamma.2 gene capping site fused to the TK gene. These results indicate that the .alpha.4 gene and the .alpha.O gene are each capable of inducing the expression of both .beta. and .gamma.2 genes resident in the environment of the cellular genome, that the active site responsible for induction is located near the N terminus of the .alpha.4 protein, and reinforce the conclusion that .gamma.2 genes resident in the environment of the host cell cannot be used to identify the authentic determinants of .gamma.2 gene regulation by currently available tests.

(PDF 0-2 workdays service)

Accession: 006288438

PMID: 3004024

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Mavromara Nazos, P.; Silver, S.; Hubenthal Voss, J., 1986: Regulation of herpes simplex virus 1 genes: a gene sequence requirements for transient induction of indicator genes regulated by b or late (c2) promoters. Virology 149: 2-64

Shapira, M.; Homa, F.L.; Glorioso, J.C.; Levine, M., 1987: Regulation of the herpes simplex virus type 1 late (gamma 2) glycoprotein C gene: sequences between base pairs -34 to +29 control transient expression and responsiveness to transactivation by the products of the immediate early (alpha) 4 and 0 genes. The glycoprotein C (gC) gene of herpes simplex virus type 1 is a true late gene, in that its expression occurs late in infection with a strict requirement for viral DNA replication. Recently, we reported on gC expression during infection with muta...

Post, L.E.; Mackem, S.; Roizman, B., 1981: Regulation of alpha genes of herpes simplex virus: expression of chimeric genes produced by fusion of thymidine kinase with alpha gene promoters. A system is reported for investigating promoters of eukaryotic cell and virus genes based on analyses of the regulation of herpes simplex virus 1 (HSV-1) thymidine kinases whose structural gene sequences are fused to the promoter of the gene under...

Sandri-Goldin, R.M.; Goldin, A.L.; Holland, L.E.; Glorioso, J.C.; Levine, M., 1983: Expression of herpes simplex virus beta and gamma genes integrated in mammalian cells and their induction by an alpha gene product. The proteins of herpes simplex virus type 1 (HSV-1) form 3 kinetic groups termed .alpha., .beta. and .gamma., whose synthesis is regulated in a cascade fashion. .alpha. products are synthesized first during infection, and they are required for syn...

Millette R.L.; Lahiri J.; Perry P.; Tomar D.; Chen S., 1988: Regulation of the early late beta gamma genes of herpes simplex virus type 1. Journal of Cellular Biochemistry Supplement (12 PART D): 214

Mackem, S.; Roizman, B., 1982: Regulation of alpha genes of herpes simplex virus: the alpha 27 gene promoter-thymidine kinase chimera is positively regulated in converted L cells. In cells infected with herpes simplex virus 1, the expression of viral genes is coordinately regulated and sequentially ordered; the alpha genes are expressed first and are followed by beta and gamma genes in a cascade fashion. Earlier, this labor...

Silver S.; Roizman B., 1985: Gamma 2 thymidine kinase chimeras are identically transcribed but regulated as gamma 2 genes in herpes simplex virus genomes and as beta genes in cell genomes. True .gamma. or .gamma.2 genes, unlike .alpha., .beta. and .gamma.1 (.beta. .gamma.) genes of herpes simplex viral 1 (HSV-1), stringently require viral DNA synthesis for their expression. The .gamma.2 genes resident in cells were induced in trans...

Silver, S.; Roizman, B., 1985: gamma 2-Thymidine kinase chimeras are identically transcribed but regulated a gamma 2 genes in herpes simplex virus genomes and as beta genes in cell genomes. True gamma or gamma 2 genes, unlike alpha, beta, and gamma 1 (beta gamma) genes of herpes simplex virus 1 (HSV-1), stringently require viral DNA synthesis for their expression. We report that gamma 2 genes resident in cells were induced in trans b...

Mavromara-Nazos, P.; Roizman, B., 1989: Delineation of regulatory domains of early (beta) and late (gamma 2) genes by construction of chimeric genes expressed in herpes simplex virus 1 genomes. The expression of the .gamma.2 class of viral genes in cells infected with herpes simplex virus 1 requires viral DNA synthesis and functional viral products made earlier in infection. To identify the sequences required for .gamma.2 gene expression...

Mills, L.K.; Shi, Y.; Millette, R.L., 1994: YY1 is the cellular factor shown previously to bind to regulatory regions of several leaky-late (beta gamma, gamma 1) genes of herpes simplex virus type 1. We have recently reported that a cellular factor, the leaky-late binding factor (LBF), binds to sites in a number of leaky-late (beta gamma or gamma 1) genes of herpes simplex virus type 1 and that an LBF site is essential for maximum viral transa...