+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Regulation of herpesvirus macro molecular synthesis part 5 properties of alpha poly peptides made in herpes simplex virus type 1 and herpes simplex virus type 2 infected cells

, : Regulation of herpesvirus macro molecular synthesis part 5 properties of alpha poly peptides made in herpes simplex virus type 1 and herpes simplex virus type 2 infected cells. Virology 77(2): 733-749

Previous reports defined as .alpha. those viral polypeptides whose production in infected [human laryngeal carcinoma HEp-2] cells does not require prior protein synthesis. Two subsequent groups, .beta. and .gamma., depend on prior .alpha. and .beta. polypeptide synthesis, respectively. Comparison of the synthesis and properties of .alpha. polypeptides specified by herpes simplex viruses (HSV) 1 and 2 showed that the 3 earliest virus-specific infected cell polypeptides (ICP) made in HSV-2 infected cells migrated slightly more slowly in polyacrylamide gels than the HSV-1 .alpha. polypeptides, ICP 4.0 and 27. Cells treated with canavanine from the time of infection with HSV-2 produced all .alpha., a subset of .beta. and a small amount of 1 .gamma. polypeptide; the synthesis of these polypeptides continued for many hours, at rates related to the multiplicity of infection. The transition from .alpha. to this subset of .beta. polypeptide synthesis did not appear to be affected by the arginine analog, but transition to the other sets of .beta. and to .gamma. polypeptide synthesis was blocked. A similar discrimination between different subsets of .beta. proteins was seen in treated HSV-1 infected cells. All .alpha. and a number of .beta. polypeptides observed underwent translocation into the nucleus, post translational modification resulting in a reduced electrophoretic mobility and phosphorylation. The modification of HSV-1 and HSV-2 ICP 4 was in at least 2 steps from the translational product ICP 4a, labeled during a pulse, to slower-migrating forms ICP 4b and 4c. All 3 forms were phosphorylated, but only 4b and 4c were found in the nucleus. In untreated infected cells ICP 4 ultimately accumulated in form ICP 4c. ICP 4a made in the presence of canavanine was not processed efficiently into ICP 4c. In another instance the polypeptide made in the presence of canavanine was not translocated into nuclei.

(PDF 0-2 workdays service)

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 006288444

PDF Full Text: Submit

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Steiner, Bret Martin, 2000: DNA polymerase from Treponema pallidum. The nucleic acid sequence encoding the gene for the DNA polymerase I enzyme of Treponema pallidum, the organism causing syphilis. Nucleic acid molecules useful as probes for detecting Treponema pallidum are described. Isolated, recombinant, and sy...

Meyer M.W.; Path M.G.; Smith K.A., 1973: Regional cephalic blood flow during sympathetic stimulation. Federation Proceedings 32(3 PART 1): 324

Valero, M.L.; Giralt, E.; Andreu, D., 1999: A comparative study of cyclization strategies applied to the synthesis of head-to-tail cyclic analogs of a viral epitope. A family of head-to-tail cyclic peptide models of the antigenic site A (G-H loop of viral protein 1) of foot-and-mouth disease virus has been designed on the basis of the three-dimensional structure adopted by the linear peptide YTASARGDLAHLTTT up...

Campbell, J.A., 1938: Box Mask for Administration of Oxygen. British Medical Journal 1(4040): 1260-1263

Morel, O.; Métrard, G.; Jeanguillaume, C.; Rakotonirina, Hé.; Blaire, T.; L.J.une, J-Jacques.; Jallet, P., 2006: Renovascular hypertension and double renal arterial supply diagnosed by Tc-99m MAG3 captopril renography. Clinical Nuclear Medicine 31(5): 298-300

Sklenský, B., 1966: Lung lesions in cast steel polishers. Vnitrni Lekarstvi 12(4): 345-351

Anonymous, 2000: An International Meeting on Candesartan cilexetil: From Concept to Clinic, Lisbon, Portugal, October 1, 1998. Candesartan is an AT1-receptor blocker used in the control of human hypertension. This meeting contains 10 papers and abstracts of 11 posters, written in English, which provide an overview of the rationale for the blockade of the renin-angiotensin...

Pang, S.; Hernandez, Y.; Feng, X.; Müllen, K., 2011: Graphene as transparent electrode material for organic electronics. Advanced Materials 23(25): 2779-2795

Meneton, P.; Bloch-Faure, M.; Hagege, A.A.; Ruetten, H.; Huang, W.; Bergaya, S.; Ceiler, D.; Gehring, D.; Martins, I.; Salmon, G.; Boulanger, C.M.; Nussberger, J.; Crozatier, B.; Gasc, J.M.; Heudes, D.; Bruneval, P.; Doetschman, T.; Ménard, J.; Alhenc-Gelas, F., 2001: Cardiovascular abnormalities with normal blood pressure in tissue kallikrein-deficient mice. Tissue kallikrein is a serine protease thought to be involved in the generation of bioactive peptide kinins in many organs like the kidneys, colon, salivary glands, pancreas, and blood vessels. Low renal synthesis and urinary excretion of tissue k...

Zhu, Y.Z.; Yang, Y.H.; Zhang, X.L., 1996: Vaginal colonization of group B Streptococcus: a study in 267 cases of factory women. An epidemiologic study on vaginal colonization of group B streptococcus (GBS) from non-pregnancy women was carried out. Two hundred sixty seven female workers were studied. The carrier rate of GBS in vaginal specimens was 10.86%. Women aged 45 yea...