+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Regulation of hexose carriers in chicken embryo fibroblasts. Effect of glucose starvation and role of protein synthesis

, : Regulation of hexose carriers in chicken embryo fibroblasts. Effect of glucose starvation and role of protein synthesis. Journal of Biological Chemistry 258(16): 9786-9792

Regulation of hexose transport was investigated in chicken embryo fibroblasts (CEF) which develop 4- to 8-fold enhanced hexose transport activity during glucose starvation. The presence of cycloheximide in low (0.5 micrograms/ml) concentrations during starvation largely blocked the enhancement of transport activity. Glucose refeeding of CEF in the starvation state led to a decline in transport to the basal level. This decline was either potentiated or blocked by the presence of cycloheximide in low or high (50 micrograms/ml) concentrations, respectively. Exposure of CEF in the fed state to low concentrations of cycloheximide resulted in a 70% decrease of transport within 6 h, whereas exposure to high concentrations of cycloheximide led to only a modest loss (35% decrease). In the glucose-starved state, CEF had no significant decline of transport when exposed to cycloheximide at either high or low concentrations. The uptake of 3-O-methylglucose by fed, starved, or cycloheximide-treated CEF correlated closely with D-glucose transport activity and [3H]cytochalasin B binding by plasma membranes prepared from CEF exposed to the same conditions. Hexose transport activity of CEF seems to largely depend on the number of functioning carriers in the plasma membrane, which apparently reflect the balance between carrier synthesis and inactivation. These two processes require protein synthesis, but are differentially sensitive to the effects of cycloheximide, such that low concentrations of cycloheximide appear to block primarily synthesis while high concentrations block both processes. Furthermore, during starvation the enhancement of transport appears largely due to decreased carrier inactivation in the face of continued carrier synthesis.

(PDF 0-2 workdays service)

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 006288454

PMID: 6885770

PDF Full Text: Submit

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Uchikawa T.; Huseby R.A.; Zain Ul Abedin M.; Samuels L.T., 1970: Changes in testicular dna produced in balb c mice by di ethyl stilbestrol. Journal of the National Cancer Institute 45(3): 525-533

Burgess E.C.; Yuill T.M., 1983: The influence of 7 environmental and physiological factors on duck plague virus shedding by carrier mallards anas platyrhynchos. Duck plague (DP) virus carrier mallards (A. platyrhynchos) were subjected to 7 environmental and physiological conditions in an attempt to stimulate DP virus shedding. The conditions were: food quality, social interaction, reproductive state, time...

Rodhain, F; Boutonnier, A; Carteron, B; Morvan, D., 1977: The culicid mosquitoes of the French Territory of Afars and Issas. 2. genus Culex. Bulletin 70 (3) 309-315

Shykind, B.M.; Kim, J.; Sharp, P.A., 1995: Activation of the TFIID-TFIIA complex with HMG-2. The nonhistone chromosomal protein HMG-2 was identified as a factor necessary for activation in a defined transcription reaction in vitro containing RNA polymerase II and purified factors. Activation occurred on all promoters assayed except that o...

Soniewski, K., 2003: Instability of phenotypes and genetic variation determine body size and body condition of lactating cows. Successful selection for milk yield in dairy cattle in the past five decades has allegedly caused deterioration in fertility, health and longevity traits. Indeed, negative energy balance typically occurring during the early stage of lactation has...

Hayashi, M., 1976: On the caudal fin skeleton of rhinogobius brunneus. Local variations in morphology and color were noted in the freshwater goby R. brunneus (Temminck et al., 1936). Since then the calculation factors for each fin and for the number of vertebrae were reported including Tukugobius flumineus Mizuno (fl...

Issenberg H.J.; Freed M.D.; Bierman F.Z.; Nadas A.S., 1980: Detection of ventricular ectopy in children with thalassemia major. Pediatric Research 14(4 PART 2): 446

Armstrong, T.W.; Caldwell, D.J.; Verma, D.K., 2005: Occupational exposure limits: an approach and calculation aid for extended work schedule adjustments. Past reviews of occupational exposure limit (OEL) adjustments have covered both decision logic and calculation methods to derive factors to assure protection of workers on extended (also known as unusual) work shifts. The approaches reviewed inclu...

Golubic, Stjepko; Le-Campion-Alsumard, Therese; Campbell, Susan, E., 1999: Diversity of marine cyanobacteria. The diversity of marine cyanobacterial species, based on field observations of natural populations in various marine environments is reviewed. The distribution of readily characterizable cyanobacterial types along gradients of ecological determina...

Tanaka, Yoshihide; Terabe, Shigeru, 1995: Partial separation zone technique for the separation of enantiomers by affinity electrokinetic chromatography with proteins as chiral pseudo-stationary phases. A previously reported technique which employed a discontinuous separation zone built in a part of a capillary was applied to the separation of enantiomers by affinity electrokinetic chromatography using proteins as chiral pseudo-stationary phases...