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Regulation of Hexose Transport in Chlorella vulgaris: Characteristics of Induction and Turnover


, : Regulation of Hexose Transport in Chlorella vulgaris: Characteristics of Induction and Turnover. Plant Physiology 53(1): 14-20

Of nine species of unicellular algae tested, Chlorella vulgaris showed the highest inducibility for an active hexose transport system. Whereas the rate of uptake in all other species was increased by induction less than 5-fold, it was increased more than 400-fold in one strain of C. vulgaris. With glucose as inducer, the minimum time necessary to synthesize inducible proteins of the transport system was 15 minutes. The Km for induction with glucose is 5 mum and with 6-deoxyglucose 1 mm. The inducing sugars have to penetrate the cells to be effective.Evidence indicating that regulation of induction occurs at the transcriptional level was obtained. The induction was inhibited by 6-methylpurine. When cells were exposed to induce in the presence of actidione no increase in transport activity could be measured. After removal of actidione as well as the inducer, an increased uptake activity was observed after 30 to 60 minutes. The induced uptake system showed a turnover with a half-life of 4 to 6 hours at 26 C under nongrowing conditions; at 0 C turnover was negligible. Turnover was partly inhibited by anaerobic condition and by actidione; it was accelerated under growing conditions.

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Accession: 006288457

PMID: 16658644

DOI: 10.1104/pp.53.1.14

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Related references

Haass, D.T.nner, W., 1974: Regulation of hexose transport in Chlorella vulgaris. Plant physiology: 53 (1) 14-20

Tanner, W.H.ass, D.D.cker, M.L.os, E.K.mor, B.K.mor, E., 1974: Active hexose transport in Chlorella vulgaris. Membrane Transport in Plants U Zimmermann and J Daintyeds: 02-208

Komor, E.; Weber, H.; Tanner, W., 1978: Essential sulfhydryl group in the transport catalyzing protein of the hexose proton co transport system of chlorella vulgaris. The polyene antibiotic nystatin transforms the sugar-proton cotransport system of Chlorella to a mere facilitated diffusion system. This experimental conditions was used to test the sugar-translocating unit of the active uptake system for possible...

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Sauer, N., 1986: Hexose-transport-deficient mutants of Chlorella vulgaris : Lack of transport activity correlates with absence of inducible proteins. Autotrophically grown cells of Chlorella vulgaris show a strong increase in the uptake rates for hexoses and for seven amino acids when incubated in the presence of hexoses. This increase is due to de-novo synthesis of three transport proteins: on...

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Komor E.; Tanner W., 1974: The hexose proton co transport system of chlorella vulgaris ph dependent change in michaelis constant values and translocation constants of the uptake system. Journal of General Physiology 64(5): 568-581