+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Regulation of human blood erythroid burst forming unit proliferation by t lymphocyte sub populations defined by fc receptors and mono clonal antibodies

, : Regulation of human blood erythroid burst forming unit proliferation by t lymphocyte sub populations defined by fc receptors and mono clonal antibodies. Blood 59(5): 990-996

To determine whether normal T-cell subpopulations influence human blood BFU-E [erythropoietic burst-forming cell] proliferation variably, sheep erythrocyte rosettable cells (unseparated T cells) were fractionated into subpopulations based on their differential binding to IgM ( cells) or IgG-coated ox erythrocytes (T.gamma. cells). For comparative purposes, T cells, T.gamma. cells and T cells depleted of T.gamma. cells (T-non-.gamma. cells) were further characterized by their reaction with the OK [hybridoma derived] panel of monoclonal antibodies (OKT3, OKT4, OKT8, OKM1). Quantities of 2.0 .times. 105, T.gamma. or T-non-.gamma. cells were mixed with 2.0 .times. 105 autologous BFU-E-enriched null cells in a methyl-cellulose culture system with 2.0 IU erythropoietin. cells or T-non-.gamma. cells consistently increased BFU-E numbers above that observed with either unseparated T cells or T.gamma. cells (P < 0.005, n = 10). The differential burst-enhancing effect of and T.gamma. cells was evident in increasing cell numbers in culture and in mixing studies. T.gamma. cells in the absence or presence of mitogens did not consistently suppress BFU-E growth below that observed in cultures with null cells alone. Monoclonal antibody analysis of T-cell subsets indicated that T.gamma. cells were enriched for OKM1+ cells and reduced in OKT3+, OKT4+ cells as compared to unseparated T cells or T-non-.gamma. cells (P < 0.05). T-non-.gamma.-cells were enriched for OKT3, OKT4+ cells and were depleted of OKM1+ cells (P < 0.05). OKT8 positivity of these cell fractions was similar. Treatment of unseparated T cells with OKT4 antibody plus complement significantly reduced the BFU-E enhancing effects of these T cells compared to complement-treated controls (P 0.005). These studies provide direct evidence for the concept of T-cell subset regulation of erythropoiesis. The variable burst-enhancing effects of, T-non-.gamma. and T.gamma. cells may be due to the presence of variable numbers of a burst-enhancing OKT4+ cell. The possibility that the differential BFU-E stimulatory effect of or T.gamma. cells is due to cellular interactions with monocytes is not excluded by these studies.

(PDF 0-2 workdays service)

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 006288514

PDF Full Text: Submit

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Goldmann, Wolfgang H.; Guttenberg, Zeno, 1998: Examination of temperature-induced 'gel-sol' transformation of alpha-actinin/cross-linked actin networks by static light scattering. We studied the gel-sol transformation of F-actin/alpha-actinin solutions. Cross-linking of actin filaments by alpha-actinin shows a temperature-dependent increase in light scatter signal, (I)T. Higher F-actin/alpha-actinin molar ratios, rAalpha as...

Rogers J.D., 1980: Hypoxylon weldenii var microsporum new variety and hypoxylon punctidiscum new species. Mycologia: 829-832

Farrell, D. A.; Greacen, E. L.; Larson, W. E., 1967: The effect of water content on axial strain in a loam soil under tension and compression. A method is described for measuring axial strain in a remolded loam soil under unconfined axial loading in both compression and tension. Using this method the stress-strain relationships for the loam soil were determined for soil-water contents ra...

Sevag, M. G.; Ross, O. A., 1944: Studies on the mechanism of the inhibitory action of zephiran on yeast cells. At 1:1,000 or higher concs. of zephiran, the absorption bands of a prepn. of cytochrome c, and of yeast cells, are either markedly faded or abolished. In manometric measurements, in a 1:35,000 dilution of zephiran, the O2 consumption of p-phenylen...

Esbjerg, N., 1936: Fertilizers for market garden crops. Comprehensive testings of the effect of stableyard manure and artificial fertilizers on the yield of different market garden crops have been carried out since 1922 at Blangsted (heavy soil) and Hornum (sandy soil). Twelve different treatments were...

Fernandez, I. J.; Rustad, L. E., 1990: Soil response to S and N treatments in a northern New England low elevation coniferous forest. A field experiment was designed to evaluate the effects of differing forms of acidifying S and N compounds on the chemistry of soils and soil solutions in a low altitude coniferous forest in northern New England. Treatments consisted of 0, 1500, 3...

Garcia Reyes, J. F.; Llorent Martinez, E. J.; Ortega Barrales, P.; Molina Diaz, A., 2006: Determination of thiabendazole residues in citrus fruits using a Multicommuted fluorescence-based optosensor. In this work, a method based on the combination of a multi-commuted flow system and solid-surface fluorescence spectroscopy has been developed and validated for the determination of thiabendazole residues in citrus fruits. The flow system was desi...

Williams, J. H.; Taylor, L.; Kuchmak, M.; Witter, R. F., 1970: Preparation of hyper cholesteremic and or hyper tri glyceridemic sera for lipid determinations. Clinica Chimica Acta 28(2): 247-253

Jiang X R.; Lin H X., 1990: Induction of differentiation in the human erythroleukemia cell line k562 by ginsenosides. Anticancer Research 10(5 PART B): 1489

Toivanen A.; Merilahti Palo R.; Gripenberg C.; Lahesmaa Rantala R.; Soderstrom K O.; Jaakkola U.M., 1987: Yersinia associated arthritis in the rat experimental model for the human reactive arthritis. Clinical Rheumatology 6(1): 112-113