EurekaMag.com logo
+ Site Statistics
References:
48,253,228
Abstracts:
22,798,842
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
EurekaMag PDF Full Text Supply ServicePDF Full Text Supply Service
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn
Follow on LinkedInFollow on Tumblr

+ Translate

Regulation of human fibroblast growth rate by both noncycling cell fraction and transition probability is shown by growth in 5 bromodeoxy uridine followed by hoechst 33258 4 5 4 methyl 1 piperazinyl 2 5' bi 1h benzimidazol 2' yl phenol tri hydro chloride flow cytometry


, : Regulation of human fibroblast growth rate by both noncycling cell fraction and transition probability is shown by growth in 5 bromodeoxy uridine followed by hoechst 33258 4 5 4 methyl 1 piperazinyl 2 5' bi 1h benzimidazol 2' yl phenol tri hydro chloride flow cytometry. Proceedings of the National Academy of Sciences of the United States of America 80(10): 2951-2955

Growth of human diploid fibroblasts in the presence of 5-bromodeoxyuridine, followed by flow cytometric analysis of DNA-specific fluorescence with Hoechst 33258 dye, allows quantitation of the proportion of cells that have not cycled, as well as those in G1 and G2 of 2 subsequent cell cycles. This technique allows rapid and accurate quantitation of the growth fraction and G1/S transition rate of these cells. The cell cycle kinetics of human diploid fibroblasts at all population doubling levels reveal 2 components: cycling cells showing a probabilistic rate of G1/S transition, and a variable proportion of noncycling cells. Both the transition probability (rate of exit from G1) and the noncycling proportion of cells change systematically as a function of serum concentration and as a function of population doubling level. The data suggest the existence of an underlying heterogeneity in the population of human diploid fibroblasts with respect to the capacity to divide in the presence of a given concentration of mitogen. Models of cell cycle kinetics must be modified to include regulation of growth by changes in the fraction of cycling cells, as well as by changes in the rate of exit from G1.

(PDF 0-2 workdays service)

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 006288524

PDF Full Text: Submit


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Barrio, A., 1967: Ethoecological observations on Hylorina sylvatica Bell (Anura, Leptodactylidae). Physis Buenos Aires, 27(No. 74: 153-157

Gong, Liang-Wei; Gao, Tian, M.; Huang, Hao; Zhuang, Zhi-Ye; Tong, Zhenqing, 2002: Transient forebrain ischemia induces persistent hyperactivity of large conductance Ca2+-activated potassium channels via oxidation modulation in rat hippocampal CA1 pyramidal neurons. The present study examined temporal changes in activity of large conductance, Ca2+-activated potassium (BKCa) channels in postischemic CA1 pyramidal neurons at 2, 6, 24 and 48 h after reperfusion. These changes in activity and possible cellular me...

Yavuz Yard?m, 2012: Electrochemical Behavior of Chlorogenic Acid at a Boron-Doped Diamond Electrode and Estimation of the Antioxidant Capacity in the Coffee Samples Based on Its Oxidation Peak. In this study, an electroanalytical methodology for the determination of chlorogenic acid (CGA) was achieved at a boron-doped diamond electrode under adsorptive transfer stripping voltammetric conditions. The values obtained for CGA were used to e...

Jose A. Cancelas; Neeta Rugg; P. Gayle Pratt; D. Nicole Worsham; Joan C. Pehta; Kate Banks; Robertson D. Davenport; W. John Judd, 2011: Infusion of P-Capt prion-filtered red blood cell products demonstrate acceptable in vivo viability and no evidence of neoantigen formation. Transmission of variant Creutzfeldt-Jacob disease (vCJD) is a major concern in blood transfusion. The P-Capt filter has been shown to remove around 4 log ID5 prion infectivity from prion-spiked human red blood cells (RBCs). Two independent, single...

Susete N. Fernandes; Yong Geng; Silvia Vignolini; Beverley J. Glover; Ana C. Trindade; João P. Canejo; Pedro L. Almeida; Pedro Brogueira; Maria H. Godinho, 2013: Structural Color and Iridescence in Transparent Sheared Cellulosic Films. Shear transparent cellulose free-standing thin films can develop iridescence similar to that found in petals of the tulip Queen of the Night. The iridescence of the film arises from the modulation of the surface into bands periodically spread perp...

Archibald, R; Mack, C., 1978: A simple and inexpensive evaporator entrainment separatory. South African sugar journal: 62 (9) 480-481

Dacey D.M.; Petersen M.R., 1991: Size stratification and morphology of the midget parasol and small bistratified ganglion cells of the human retina. Society for Neuroscience Abstracts 17(1-2): 343

Al-Faqih, Laith; Johnson, Pauline, D.; Allen, Stephen, J., 2008: Evaluation of a new peat-based sorbent for metals capture. A new peat-based sorbent was evaluated for the capture of heavy metals from waste streams. The media is a pelletted blend of organic humic material targeted for the capture of soluble metals from industrial waste streams and stormwater. The metals...

Cantet, R. J. C.; Birchmeier, A. N.; Steibel, J. P., 2004: Full conjugate analysis of normal multiple traits with missing records using a generalized inverted Wishart distribution. A Markov chain Monte Carlo (MCMC) algorithm to sample an exchangeable covariance matrix, such as the one of the error terms (R0) in a multiple trait animal model with missing records under normal-inverted Wishart priors is presented. The algorithm...

Nachkov, D., 1971: Removal of polyphenols from wine. Lozarstvo i vinarstvo: 20 (8) 25-28