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Regulation of human in vitro anti hapten responses demonstration of a carrier effect


Cellular Immunology 71(1): 110-117
Regulation of human in vitro anti hapten responses demonstration of a carrier effect
Human peripheral blood mononuclear cells, tonsillar lymphocytes or mixtures of allogeneic or autologous B and T cells from these tissues were stimulated in vitro with the soluble hapen:carrier complexes TNP-OA [trinitrophenyl-ovalbumin], TNP-KLH [TNP-Keyhole limpet hemocyanin], TNP-Myo [TNP-myoglobin] or TNP-Lac [TNP-.beta.-lactoglobulin]. These complexes were able to induce TNP-specific, direct PFC [plague forming cells] during 5-6 days in culture. The response involved proliferating PFC precursor B cells, nonproliferating T helper cells and radiosensitive T-suppressor cell precursors. Exposure to high concentrations of free or carrier-bound hapten resulted in the inactivation of PFC precursor B cells. Carrier specific suppressor T cells could be induced by priming with nonhaptenated carrier protein and were able to block the PFC response when added to fresh target cultures. The use of hapten:carrier complexes permits the characterization of distinct T-cell functions independently of the assay system for B-cell activation.


Accession: 006288539

PMID: 6754097



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