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Regulation of human in vitro anti hapten responses demonstration of a carrier effect


, : Regulation of human in vitro anti hapten responses demonstration of a carrier effect. Cellular Immunology 71(1): 110-117

Human peripheral blood mononuclear cells, tonsillar lymphocytes or mixtures of allogeneic or autologous B and T cells from these tissues were stimulated in vitro with the soluble hapen:carrier complexes TNP-OA [trinitrophenyl-ovalbumin], TNP-KLH [TNP-Keyhole limpet hemocyanin], TNP-Myo [TNP-myoglobin] or TNP-Lac [TNP-.beta.-lactoglobulin]. These complexes were able to induce TNP-specific, direct PFC [plague forming cells] during 5-6 days in culture. The response involved proliferating PFC precursor B cells, nonproliferating T helper cells and radiosensitive T-suppressor cell precursors. Exposure to high concentrations of free or carrier-bound hapten resulted in the inactivation of PFC precursor B cells. Carrier specific suppressor T cells could be induced by priming with nonhaptenated carrier protein and were able to block the PFC response when added to fresh target cultures. The use of hapten:carrier complexes permits the characterization of distinct T-cell functions independently of the assay system for B-cell activation.

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Accession: 006288539

PMID: 6754097

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Related references

Katz, D.H.; Davie, J.M.; Paul, W.E.; Benacerraf, B., 1971: Carrier function in anti-hapten antibody responses. IV. Experimental conditions for the induction of hapten-specific tolerance or for the stimulation of anti-hapten anamnestic responses by "nonimmunogenic" hapten-polypeptide conjugates. Administration of nonimmunogenic 2,4-dinitrophenyl (DNP) conjugates of copolymers of D or L-glutamic acid and lysine (GL) induces hapten-specific tolerance in nonimmune and DNP-ovalbumin-primed strain 13 guinea pigs. This tolerant state is evidenc...

Paul, W.E.; Katz, D.H.; Goidl, E.A.; Benacerraf, B., 1970: Carrier function in anti hapten immune responses part 2 specific properties of carrier cells capable of enhancing anti hapten antibody responses. Journal of Experimental Medicine 132(2): 283-299

Paul, W.E.; Katz, D.H.; Goidl, E.A.; Benacerraf, B., 1970: Carrier function in anti-hapten immune responses. II. Specific properties of carrier cells capable of enhancing anti-hapten antibody responses. Preimmunization enhanced both the primary and the secondary anti-DNP response, particularly when antigen was given in Freund's complete adjuvant. The effect could not be passively transferred with anti-BGG serum. The carrier specificity of ha...

Katz, D.H.; Paul, W.E.; Goidl, E.A.; Benacerraf, B., 1970: Carrier function in anti hapten immune responses part 1 enhancement of primary and secondary anti hapten antibody responses by carrier pre immunization. Journal of Experimental Medicine 132(2): 261-282

Katz, D.H.; Paul, W.E.; Goidl, E.A.; Benacerraf, B., 1970: Carrier function in anti-hapten immune responses. I. Enhancement of primary and secondary anti-hapten antibody responses by carrier preimmunization. Preimmunization of either guinea pigs or rabbits to bovine gamma globulin (BGG) prepares the animals for markedly enhanced antibody responses to 2,4-dinitrophenyl-BGG (DNP-BGG). This phenomenon is observed both in the primary anti-DNP antibody res...

Nagase, F.; Nakashima, I.; Kato, N., 1979: Studies on the immune response in chickens. III. Effect of substitution of carrier on elicitation of anti-hapten antibody responses and generation of hapten-specific memory. The role of carrier in both elicitation of anti-hapten antibody responses and generation of hapten-specific memory in chickens were studied using dinitrophenyl (DNP) conjugate of T-independent antigen carrier (DNP--Ficoll), that of isologous serum...

Nagase F.; Nakashima I.; Kato N., 1979: Studies on the immune response in chickens part 3 effect of substitution of carrier on elicitation of anti hapten antibody responses and generation of hapten specific memory. The role of carrier in both elicitation of anti-hapten antibody responses and generation of hapten-specific memory in chickens were studied using dinitrophenyl (DNP) conjugate of T[thymus-derived]-independent antigen carrier (DNP-Ficoll), isologou...

Mabuchi A.; Yokomuro K.; Kimura Y., 1977: Specificity of delayed hyper sensitivity and carrier effect in anti hapten immune response in guinea pigs immunized with hapten homologous carrier conjugates. Japanese Journal of Allergology 26(9): 663-664

Blaser, K.; Nakagawa, T.; D.W.ck, A.L., 1983: Effect of passively administered isologous anti-idiotypes directed against anti-carrier (ovalbumin) antibodies on the anti-hapten IgE and IgG antibody responses in BALB/c mice. Recently we have shown that active immunization of syngenic animals with anti-ovalbumin antibodies evokes an anti-idiotypic (aId) response, which in consequence leads to suppression of the anti-hapten (benzylpenicilloyl, BPO; dinitrophenyl, DNP) I...

Kitagawa M., 1974: Induction of anti hapten antibody response by hapten isologous carrier conjugate part 1 development of hapten reactive helper cells by hapten isologous carrier. Cellular Immunology: 182-192